Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,387,080	A→G	V764A (GTC→GCC)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,387,080	0	A	G	100.0%	302.1 / NA	86	V764A (GTC→GCC)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/49);  total (37/49)

TCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGACTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTGGATCAGCATCCAGCGGCG  >  NC_000913/4386945‑4387216                                                                                                                                        |                                                                                                                                         tCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTc                                                                                                                                      >  1:992457/1‑140 (MQ=255)  cGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCa                                                                                                                                     <  2:619222/140‑1 (MQ=255)      gAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTg                                                                                                                                 >  1:12727/1‑140 (MQ=255)      gAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTg                                                                                                                                 >  1:582394/1‑140 (MQ=255)      gAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTg                                                                                                                                 <  1:1403482/140‑1 (MQ=255)        aGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCt                                                                                                                               <  1:401771/140‑1 (MQ=255)        aGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCt                                                                                                                               <  1:1130541/140‑1 (MQ=255)        aGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCt                                                                                                                               <  2:162246/140‑1 (MQ=255)        aGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCt                                                                                                                               <  1:993220/140‑1 (MQ=255)         gCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTc                                                                                                                              >  2:1092691/1‑140 (MQ=255)            gcaggCGAAATGGGTTTCTGGCCACAACAGAATGACAGCAAGCAGGCGTACCGACAGCAAAATTGAGCGCGCTAACCGCAAGGATTGCGCACTGATGGCATCGAGATCGCCTTCGCTTTCATCGGGTTCAATTGccccct                                                                                                                           <  2:674906/136‑3 (MQ=255)            gAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCtt                                                                                                                           <  1:38612/140‑1 (MQ=255)               aGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCg                                                                                                                        <  2:12727/140‑1 (MQ=255)                 cGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCggg                                                                                                                      <  2:624394/140‑1 (MQ=255)                 cGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCggg                                                                                                                      <  1:1333468/140‑1 (MQ=255)                 cGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCggg                                                                                                                      <  1:976944/140‑1 (MQ=255)                  ggAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGc                                                                                                                     <  2:848936/140‑1 (MQ=255)                  ggAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGc                                                                                                                     <  1:39697/140‑1 (MQ=255)                               gACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGatgatg                                                                                                        <  1:946578/140‑1 (MQ=255)                                aCCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCg                                                                                                                        <  1:504721/123‑1 (MQ=255)                                aCCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCg                                                                                                                        >  2:504721/1‑123 (MQ=255)                                aCCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATgc                                                                                                       >  1:15556/1‑140 (MQ=255)                                aCCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATgc                                                                                                       >  1:119714/1‑140 (MQ=255)                                  cacaGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATgcgc                                                                                                     <  2:582394/140‑1 (MQ=255)                                   acaGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCt                                                                                                    <  2:1353134/140‑1 (MQ=255)                                   acaGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCt                                                                                                    <  2:744119/140‑1 (MQ=255)                                    caGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCtt                                                                                                   >  1:1407850/1‑140 (MQ=255)                                     aGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGtgatga                                                                                                          >  2:1241641/1‑132 (MQ=255)                                     aGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGtgatga                                                                                                          <  1:1241641/132‑1 (MQ=255)                                     aGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTc                                                                                                  >  2:1155163/1‑140 (MQ=255)                                       cacaATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCct                                                                                                >  2:667168/1‑140 (MQ=255)                                       cacaATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCct                                                                                                >  2:86383/1‑140 (MQ=255)                                         caATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCctct                                                                                              <  2:1432390/140‑1 (MQ=255)                                          aaTGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCtt                                                                                             <  1:1092691/140‑1 (MQ=255)                                             gACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGc                                                                                          <  2:992457/140‑1 (MQ=255)                                               cAGAAAGCAGGGCGATCAACATCAAAATTGAGCGC‑CCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCAc                                                                                       >  2:1334/1‑140 (MQ=255)                                                 gAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATAGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCAcg                                                                                      >  2:1058907/1‑140 (MQ=255)                                                  aaaGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCctct                                                                                              >  2:591876/1‑131 (MQ=255)                                                  aaaGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCctct                                                                                              <  1:591876/131‑1 (MQ=255)                                                  aaaGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCAcgc                                                                                     >  1:972893/1‑140 (MQ=255)                                                      cAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCAc                                                                                 >  1:106187/1‑140 (MQ=255)                                                      cAGGGCGATCAACATCAAAATTGAGCGC‑CCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACg                                                                                <  1:1334/140‑1 (MQ=255)                                                       aGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACg                                                                                <  1:86383/140‑1 (MQ=255)                                                       aGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACg                                                                                <  1:551219/140‑1 (MQ=255)                                                       aGGGCGATCAACATCAAAATGGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACg                                                                                <  2:664876/140‑1 (MQ=255)                                                        gggCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGt                                                                               <  2:106187/140‑1 (MQ=255)                                                         ggCGATCAACATCAAAATTGGGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGtt                                                                              >  1:27736/1‑140 (MQ=255)                                                         ggCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGtt                                                                              >  2:946667/1‑140 (MQ=255)                                                           cGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTgc                                                                            <  2:1403833/140‑1 (MQ=255)                                                              tCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGcc                                                                                         <  1:299924/124‑1 (MQ=255)                                                              tCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGcc                                                                                         >  2:299924/1‑124 (MQ=255)                                                                aaCATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTaa                                                                       <  2:119714/140‑1 (MQ=255)                                                                    tCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACAtc                                                                   >  1:501043/1‑140 (MQ=255)                                                                          ttGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGcc                                                             >  2:65360/1‑140 (MQ=255)                                                                          ttGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGcc                                                             <  1:1058907/140‑1 (MQ=255)                                                                             aGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTgcg                                                                           <  2:660281/123‑1 (MQ=255)                                                                             aGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTgcg                                                                           >  1:660281/1‑123 (MQ=255)                                                                             aGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCgg                                                          >  2:1027099/1‑140 (MQ=255)                                                                              gcgcACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCgga                                                         <  2:1407850/140‑2 (MQ=255)                                                                              gcgcACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGc                                                         <  1:65360/140‑1 (MQ=255)                                                                                  aCCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTg                                                               >  2:316777/1‑130 (MQ=255)                                                                                  aCCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTg                                                               <  1:316777/130‑1 (MQ=255)                                                                                  aCCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGcc                                                             >  2:531996/1‑132 (MQ=255)                                                                                  aCCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGcc                                                             <  1:531996/132‑1 (MQ=255)                                                                                         gCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGcc                                              <  2:15556/140‑1 (MQ=255)                                                                                         gCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGcc                                              <  2:972893/140‑1 (MQ=255)                                                                                            aGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGa                                           >  2:443299/1‑140 (MQ=255)                                                                                              gATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATc                                         <  1:1393931/140‑1 (MQ=255)                                                                                                 tGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCaaa                                      >  1:371712/1‑140 (MQ=255)                                                                                                 tGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCAAGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCaaa                                      >  2:946991/1‑140 (MQ=255)                                                                                                   cgcACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACg                                    >  1:798509/1‑140 (MQ=255)                                                                                                    gcACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGc                                   <  2:798509/140‑1 (MQ=255)                                                                                                     cACTGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGcc                                  >  2:1420896/1‑140 (MQ=255)                                                                                                        tGATGGCATCGAGATCTACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGc                               >  2:91937/1‑140 (MQ=255)                                                                                                        tGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGc                               >  2:1321037/1‑140 (MQ=255)                                                                                                        tGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGc                               >  1:402904/1‑140 (MQ=255)                                                                                                        tGATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGc                               >  1:935763/1‑140 (MQ=255)                                                                                                         gATGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGcc                              >  1:1310648/1‑140 (MQ=255)                                                                                                           tGGCATCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTg                            <  1:667168/140‑1 (MQ=255)                                                                                                               aTCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTgcggc                        <  1:1027099/140‑1 (MQ=255)                                                                                                                tCGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTgcggcg                       <  1:946667/140‑1 (MQ=255)                                                                                                                 cGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGgcgc                      <  2:935763/140‑1 (MQ=255)                                                                                                                 cGAGATCGACTTCGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGgcgc                      <  1:1018083/140‑1 (MQ=255)                                                                                                                             cGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTg                    >  2:821712/1‑130 (MQ=255)                                                                                                                             cGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTg                    <  1:1516358/130‑1 (MQ=255)                                                                                                                             cGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTg                    >  2:1516358/1‑130 (MQ=255)                                                                                                                             cGCTTTCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTg                    <  1:821712/130‑1 (MQ=255)                                                                                                                                 ttCATCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTGGATCAGCATCCAgc      <  1:91937/140‑1 (MQ=255)                                                                                                                                     tCGGCTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTGGATCAGCATCCAgcggcg  <  2:1072072/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATCAACATCAAAATTGAGCGCACCAGCCGCAAGGATTGCGCACTGATGGCATCGAGATCGACTTCGCTTTCATCGACTTCAATTGCTCCTTCCGGGCTACTGTGATGATGCGCTTCCTCTTCGCCACGCGCACGTTGCGCTAACATCTCTGCCCGGCGATGCTTCGCCCGATCAAACGCCAGCCTGCGGCGCTGGATCAGCATCCAGCGGCG  >  NC_000913/4386945‑4387216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑