Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,387	A→G	E110E (GAA→GAG)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,387	0	A	G	100.0%	192.5 / NA	55	E110E (GAA→GAG)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

ACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTC  >  NC_000913/4451250‑4451519                                                                                                                                          |                                                                                                                                     aCCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGcc                                                                                                                                    >  1:1331713/1‑140 (MQ=255)   cATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  >  2:44970/1‑140 (MQ=255)    aTCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaa                                                                                                                                 <  1:151100/140‑1 (MQ=255)     tCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaaa                                                                                                                                >  2:1338046/1‑140 (MQ=255)     tCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCaaa                                                                                                                                >  1:90709/1‑140 (MQ=255)        ggctggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGc                                                                                                                             >  2:1342949/1‑140 (MQ=255)          ctggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCtt                                                                                                                           <  2:989493/140‑1 (MQ=255)            ggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCACCCTGCTACCCAATATGTCTGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCg                                                                                                                         <  2:1366164/140‑1 (MQ=255)            ggAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCg                                                                                                                         <  1:686647/140‑1 (MQ=255)               aGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGcc                                                                                                                      >  1:802310/1‑140 (MQ=255)                   cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  >  2:529436/1‑124 (MQ=255)                   cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCa                                                                                                                                  <  1:529436/124‑1 (MQ=255)                   cAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCt                                                                                                                  >  1:746826/1‑140 (MQ=255)                       cTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTAcgc                                                                                                              <  1:1342949/140‑1 (MQ=255)                           ctcACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCa                                                                                                          <  1:1338046/140‑1 (MQ=255)                            tcACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCaa                                                                                                         <  2:799821/140‑1 (MQ=255)                              aCCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAag                                                                                                       <  2:802310/140‑1 (MQ=255)                                cGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAagag                                                                                                     >  1:593458/1‑140 (MQ=255)                                      aTACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGaa                                                                                               <  2:849422/140‑1 (MQ=255)                                           gCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAgcg                                                                                          <  1:1431739/140‑1 (MQ=255)                                                 gcgcAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCAc                                                                                    >  1:1257915/1‑140 (MQ=255)                                                     aacaacTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGaa                                                                                <  1:1325996/140‑1 (MQ=255)                                                         acTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGa                                                                            <  2:1282934/140‑1 (MQ=255)                                                           tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  2:608533/140‑1 (MQ=255)                                                           tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  2:1257915/140‑1 (MQ=255)                                                           tCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATg                                                                          <  1:1342068/140‑1 (MQ=255)                                                                  tGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCtt                                                                   <  1:150551/140‑1 (MQ=255)                                                                   ggCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTa                                                                  <  2:1331713/140‑1 (MQ=255)                                                                       ccGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGt                                                              <  2:746826/140‑1 (MQ=255)                                                                         gTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGttt                                                            >  2:465193/1‑140 (MQ=255)                                                                           cTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTtct                                                          <  2:90709/140‑1 (MQ=255)                                                                              tCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTccc                                                       >  1:1199256/1‑140 (MQ=255)                                                                                    aGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACg                                                 >  1:829715/1‑140 (MQ=255)                                                                                     gTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGt                                                >  1:1159910/1‑140 (MQ=255)                                                                                       cAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTgc                                              <  2:593458/140‑1 (MQ=255)                                                                                          ccTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGcgcg                                           <  1:912532/140‑1 (MQ=255)                                                                                                aCCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGc                                     >  2:1128308/1‑140 (MQ=255)                                                                                                    aaTATGTCGTTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACCGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCaa                                 >  1:1280739/1‑140 (MQ=255)                                                                                                      tatGTCGGTCGCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAAcc                               >  1:1458257/1‑140 (MQ=255)                                                                                                           cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  2:778275/1‑140 (MQ=255)                                                                                                           cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  1:1354401/1‑140 (MQ=255)                                                                                                           cggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                          >  2:511684/1‑140 (MQ=255)                                                                                                            ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGaa                                         <  2:1394766/124‑1 (MQ=255)                                                                                                            ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGaa                                         >  1:1394766/1‑124 (MQ=255)                                                                                                            ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCtttt                         >  1:867347/1‑140 (MQ=255)                                                                                                            ggtcgCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCtttt                         >  1:1528118/1‑140 (MQ=255)                                                                                                                gCTGATAATCTATTTATAGGCCGCGAGCCCGAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGt                     >  1:852763/1‑140 (MQ=255)                                                                                                                gCTGATAATCTATTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGt                     >  1:1236545/1‑140 (MQ=255)                                                                                                                           aTTTATAGGCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATgcagc          >  1:268943/1‑140 (MQ=255)                                                                                                                                  ggCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   <  2:867347/140‑1 (MQ=255)                                                                                                                                   gCCGCTAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgtc  >  1:794427/1‑140 (MQ=255)                                                                                                                                   gCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTcg                   <  2:239765/123‑1 (MQ=255)                                                                                                                                   gCCGCGAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTcg                   >  1:239765/1‑123 (MQ=255)                                                                                                                                      gcgAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   <  2:1166584/136‑1 (MQ=255)                                                                                                                                      gcgAGCCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt   >  1:1166584/1‑136 (MQ=255)                                                                                                                                          |                                                                                                                                     ACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTC  >  NC_000913/4451250‑4451519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑