Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,298	A→G	F328L (TTC→CTC)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,298	0	A	G	100.0%	298.5 / NA	86	F328L (TTC→CTC)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/42);  total (44/42)

CTCCATCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCA  >  NC_000913/4590161‑4590429                                                                                                                                          |                                                                                                                                    cTCCATCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCa                                                                                                                                   <  1:734968/140‑1 (MQ=255)  tCCATCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCag                                                                                                                                  >  1:440526/1‑140 (MQ=255)    cATCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCagag                                                                                                                                <  1:677425/140‑1 (MQ=255)      tCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAgcg                                                                                                                              <  1:386395/140‑1 (MQ=255)       cAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAgcgc                                                                                                                             <  2:469055/140‑1 (MQ=255)       cAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAgcgc                                                                                                                             <  2:469054/140‑1 (MQ=255)       cAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAgcgc                                                                                                                             >  1:152041/1‑140 (MQ=255)         gCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCt                                                                                                                           >  2:930258/1‑140 (MQ=255)         gCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCt                                                                                                                           >  2:1176506/1‑140 (MQ=255)         gCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCt                                                                                                                           >  2:756096/1‑140 (MQ=255)         gCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCt                                                                                                                           >  1:438206/1‑140 (MQ=255)            ttGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTc                                                                                                                        >  2:1386767/1‑140 (MQ=255)            ttGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTc                                                                                                                        <  1:533894/140‑1 (MQ=255)              gcgcCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCca                                                                                                                      >  1:1049799/1‑140 (MQ=255)              gcgcCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCca                                                                                                                      >  1:464579/1‑140 (MQ=255)               cgcCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCcac                                                                                                                     >  1:400780/1‑140 (MQ=255)               cgcCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCcac                                                                                                                     >  1:1451826/1‑140 (MQ=255)               cgcCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCcac                                                                                                                     >  1:805719/1‑140 (MQ=255)               cgcCGTAGCCGATGAAACGCGCGTCGGTTTAATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCcac                                                                                                                     >  2:1101097/1‑140 (MQ=255)                 ccGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAg                                                                                                                   <  2:593337/140‑1 (MQ=255)                 ccGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAg                                                                                                                   <  1:1401033/140‑1 (MQ=255)                 ccGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAg                                                                                                                   <  2:887311/140‑1 (MQ=255)                 ccGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAg                                                                                                                   <  2:1023552/140‑1 (MQ=255)                 ccGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAg                                                                                                                   <  2:152041/140‑1 (MQ=255)                   gTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCg                                                                                                                 <  2:438206/140‑1 (MQ=255)                     aGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCggg                                                                                                               <  2:805719/140‑1 (MQ=255)                      gCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGc                                                                                                              >  2:1170681/1‑140 (MQ=255)                      gCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGc                                                                                                              <  1:1176506/140‑1 (MQ=255)                         gATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAg                                                                                                           <  1:1386767/140‑1 (MQ=255)                           tGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGc                                                                                                              <  2:762436/135‑1 (MQ=255)                           tGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGc                                                                                                              >  1:762436/1‑135 (MQ=255)                            gAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCTCCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTAc                                                                                                        <  1:448105/140‑1 (MQ=255)                            gAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTAc                                                                                                        <  2:367699/140‑1 (MQ=255)                            gAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTAc                                                                                                        <  1:1311078/140‑1 (MQ=255)                             aaaCGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTAcc                                                                                                       <  1:1367644/140‑1 (MQ=255)                                 gcgcgTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCa                                                                                                   <  1:756096/140‑1 (MQ=255)                                 gcgcgTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCa                                                                                                   >  2:481714/1‑140 (MQ=255)                                 gcgcgTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCa                                                                                                   <  1:930258/140‑1 (MQ=255)                                      tCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTa                                                                                              <  2:61520/140‑1 (MQ=255)                                      tCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTa                                                                                              <  1:98624/140‑1 (MQ=255)                                      tCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTa                                                                                              <  1:481714/140‑1 (MQ=255)                                               ttAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCAt                                                                                     >  2:718780/1‑140 (MQ=255)                                                   ccAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggca                                                                                 >  2:1448961/1‑140 (MQ=255)                                                   ccAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggca                                                                                 >  2:782770/1‑140 (MQ=255)                                                    cagcagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcag                                                                                >  2:1401017/1‑140 (MQ=255)                                                       cagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcaggca                                                                             >  2:747808/1‑140 (MQ=255)                                                       cagTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATggcaggca                                                                             >  2:1282632/1‑140 (MQ=255)                                                         gTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCAtt                                                                           >  2:196475/1‑140 (MQ=255)                                                            ttGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTc                                                                        >  1:175792/1‑140 (MQ=255)                                                             tGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCa                                                                       <  2:1377554/140‑1 (MQ=255)                                                                          gAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAg                                                          <  2:440526/140‑1 (MQ=255)                                                                          gAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAg                                                          >  2:479614/1‑140 (MQ=255)                                                                          gAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAg                                                          <  2:1451826/140‑1 (MQ=255)                                                                            agagATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGca                                                        <  2:1503374/140‑1 (MQ=255)                                                                              agaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcacc                                                      <  1:747808/140‑1 (MQ=255)                                                                               gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcacca                                                     >  2:1214333/1‑140 (MQ=255)                                                                               gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcacca                                                     >  2:1499787/1‑140 (MQ=255)                                                                               gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcacca                                                     <  2:175792/140‑1 (MQ=255)                                                                               gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcacca                                                     >  1:466352/1‑140 (MQ=255)                                                                                aTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcaccac                                                    >  2:827049/1‑140 (MQ=255)                                                                                  cAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGca                                                        >  2:602193/1‑134 (MQ=255)                                                                                  cAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGca                                                        <  1:602193/134‑1 (MQ=255)                                                                                     cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGca                                                        >  2:780598/1‑131 (MQ=255)                                                                                     cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGca                                                        <  1:780598/131‑1 (MQ=255)                                                                                     cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATAc                                               >  1:996886/1‑140 (MQ=255)                                                                                      gcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATAcc                                              <  2:1049799/140‑1 (MQ=255)                                                                                         tGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAg                                           >  2:1264388/1‑140 (MQ=255)                                                                                         tGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAg                                           >  2:456172/1‑140 (MQ=255)                                                                                         tGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGAACCACGATACCCAg                                           >  2:942395/1‑140 (MQ=255)                                                                                           gAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAgcg                                         >  1:1348139/1‑140 (MQ=255)                                                                                             aGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGcc                                       <  2:464579/140‑1 (MQ=255)                                                                                             aGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGcc                                       <  1:1170681/140‑1 (MQ=255)                                                                                                  gATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGcc                                  <  1:1460851/140‑1 (MQ=255)                                                                                                       cGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCcgacga                             <  1:718780/140‑1 (MQ=255)                                                                                                       cGCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCcgacga                             >  1:660402/1‑140 (MQ=255)                                                                                                        gCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGAt                            <  1:942395/140‑1 (MQ=255)                                                                                                        gCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGAt                            <  1:1264388/140‑1 (MQ=255)                                                                                                        gCACCACAGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGAt                            <  1:456172/140‑1 (MQ=255)                                                                                                            cacaGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACg                        >  1:873710/1‑140 (MQ=255)                                                                                                              caGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGcc                      >  1:1105440/1‑140 (MQ=255)                                                                                                              caGGCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGcc                      >  2:1467043/1‑140 (MQ=255)                                                                                                                 gCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAt                   >  1:682763/1‑140 (MQ=255)                                                                                                                 gCGATGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAt                   >  2:1248915/1‑140 (MQ=255)                                                                                                                    aTGGTGATGAACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAtttt                >  2:792824/1‑140 (MQ=255)                                                                                                                          atgaACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGaa          <  1:1401017/140‑1 (MQ=255)                                                                                                                           tgaACAGGAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAg         >  2:621758/1‑140 (MQ=255)                                                                                                                                  gAACGGGAGCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCa  >  1:39017/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CTCCATCAGCATTGCGCCGTAGCCGATGAAACGCGCGTCGGTTTCATTAGCCAGCAGTTTTGGCGTCGTACCGGAAGAGATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCA  >  NC_000913/4590161‑4590429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑