Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	705,951	T→A	intergenic (+61/‑142)	nagE → / → glnS	N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	705,951	0	T	A	100.0%	104.2 / NA	31	intergenic (+61/‑142)	nagE/glnS	N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/16);  total (15/16)

CAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTT  >  NC_000913/705840‑706079                                                                                                                |                                                                                                                                 cAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCatcaat                                                                                                      <  1:1452661/140‑1 (MQ=255)             gtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAAt                                                                                          <  1:1149336/140‑1 (MQ=255)             gtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAAt                                                                                          <  1:127443/140‑1 (MQ=255)             gtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAAt                                                                                          <  1:1482889/140‑1 (MQ=255)             gtgGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTACGCCTGATGCGACGCTTTAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAAt                                                                                          <  1:98395/140‑1 (MQ=255)                 cGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGGGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCACAATCCtt                                                                                      <  1:534010/140‑1 (MQ=255)                               cTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCCGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAAt                                                                        >  1:1186440/1‑140 (MQ=255)                                       aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaa                                                                >  1:1034325/1‑140 (MQ=255)                                       aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaa                                                                >  1:379304/1‑140 (MQ=255)                                       aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCAAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaa                                                                >  2:1403174/1‑140 (MQ=255)                                            aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTaa                                                           >  1:1530041/1‑140 (MQ=255)                                             aaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCCGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc                                                          <  2:1186440/140‑1 (MQ=255)                                              aaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACa                                                         <  1:451581/140‑1 (MQ=255)                                                       gCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGc                                                >  2:1146356/1‑140 (MQ=255)                                                           tATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGt                                            >  2:1528845/1‑140 (MQ=255)                                                            aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                           <  2:1007832/140‑1 (MQ=255)                                                            aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                           <  2:152937/140‑1 (MQ=255)                                                            aTGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTc                                           <  1:1146356/140‑1 (MQ=255)                                                                  gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                          >  2:942844/1‑135 (MQ=255)                                                                  gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTcc                                          <  1:942844/135‑1 (MQ=255)                                                                  gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCtt                                     >  1:400443/1‑140 (MQ=255)                                                                             tGAGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATa                          >  2:552715/1‑140 (MQ=255)                                                                               aGCGTCGCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                        >  2:1486207/1‑140 (MQ=255)                                                                                     gCATCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTta                  >  1:709008/1‑140 (MQ=255)                                                                                        tCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATAc               <  2:400443/140‑1 (MQ=255)                                                                                        tCCAACAATGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATAc               <  1:1489596/140‑1 (MQ=255)                                                                                                tGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTTATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTa       <  1:859471/140‑1 (MQ=255)                                                                                                tGACAAGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTa       >  2:652306/1‑140 (MQ=255)                                                                                                    aaGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgt             >  1:377998/1‑130 (MQ=255)                                                                                                    aaGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACgt             <  2:377998/130‑1 (MQ=255)                                                                                                     aGCGGTGGAGAACTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCtt  >  2:1381314/1‑140 (MQ=255)                                                                                                                |                                                                                                                                 CAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTT  >  NC_000913/705840‑706079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑