Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	869,681	A→G	K91E (AAA→GAA)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	869,681	0	A	G	100.0%	269.4 / NA	77	K91E (AAA→GAA)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/38);  total (39/38)

GCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGG  >  NC_000913/869546‑869816                                                                                                                                        |                                                                                                                                        gCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAAtt                                                                                                                                     >  1:1205317/1‑140 (MQ=255)   aaaTGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGc                                                                                                                                   >  1:973898/1‑140 (MQ=255)   aaaTGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCATTTCCGATGACGGCATTACTTACACCGTGGAATTGc                                                                                                                                   >  2:376298/1‑140 (MQ=255)       gACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGa                                                                                                                               <  1:971790/140‑1 (MQ=255)          acGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAgg                                                                                                                            <  2:437768/140‑1 (MQ=255)             ttATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCAt                                                                                                                         <  2:930956/140‑1 (MQ=255)              tATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCAtt                                                                                                                        <  1:364771/140‑1 (MQ=255)               aTCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTa                                                                                                                       <  1:1120881/140‑1 (MQ=255)               aTCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTa                                                                                                                       <  1:587028/140‑1 (MQ=255)                 ctcAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCggg                                                                                                                                >  2:655041/1‑129 (MQ=255)                 ctcAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCggg                                                                                                                                <  1:655041/129‑1 (MQ=255)                 ctcAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTaaa                                                                                                                     >  1:1420744/1‑140 (MQ=255)                    aGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTc                                                                                                                  >  2:422126/1‑140 (MQ=255)                    aGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTc                                                                                                                  <  2:1205317/140‑1 (MQ=255)                    aGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTc                                                                                                                  <  1:658743/140‑1 (MQ=255)                      gCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCa                                                                                                                >  1:710470/1‑140 (MQ=255)                      gCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCa                                                                                                                <  1:913923/140‑1 (MQ=255)                      gCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCa                                                                                                                >  2:628915/1‑140 (MQ=255)                          tAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTa                                                                                                                       <  2:1438982/129‑1 (MQ=255)                          tAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTa                                                                                                                       >  1:1438982/1‑129 (MQ=255)                          tAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGAt                                                                                                            >  1:466599/1‑140 (MQ=255)                          tAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGACATTAAATTCCAAGAt                                                                                                            >  1:125474/1‑140 (MQ=255)                           aGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATg                                                                                                           <  2:979886/140‑1 (MQ=255)                              gAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCa                                                                                                        >  2:396104/1‑140 (MQ=255)                              gAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCa                                                                                                        <  1:270504/140‑1 (MQ=255)                                aaTCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCAcc                                                                                                      <  1:396104/140‑1 (MQ=255)                                              gggCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAAcgccgc                                                                                        >  1:1192882/1‑140 (MQ=255)                                                    ttCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGt                                                                                  >  2:1438749/1‑140 (MQ=255)                                                         tCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAg                                                                             <  2:973898/140‑1 (MQ=255)                                                         tCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAg                                                                             <  1:1438749/140‑1 (MQ=255)                                                         tCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAg                                                                             >  2:818685/1‑140 (MQ=255)                                                            ggATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGa                                                                          >  2:1146168/1‑140 (MQ=255)                                                             gATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGaa                                                                         <  2:466599/140‑1 (MQ=255)                                                              aTAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAAt                                                                        >  2:751870/1‑140 (MQ=255)                                                                   gagaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGa                                                                   >  2:1413680/1‑140 (MQ=255)                                                                    agaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGAc                                                                  <  2:1420744/140‑1 (MQ=255)                                                                    agaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGAc                                                                  <  1:628915/140‑1 (MQ=255)                                                                    agaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGAc                                                                  <  1:1146168/140‑1 (MQ=255)                                                                     gaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGAcc                                                                 >  1:634923/1‑140 (MQ=255)                                                                       tGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAAt                                                                        >  2:630300/1‑131 (MQ=255)                                                                       tGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAAt                                                                        <  1:630300/131‑1 (MQ=255)                                                                         aaaCTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGc                                                             <  1:376298/140‑1 (MQ=255)                                                                           aCTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCa                                                           >  1:178470/1‑140 (MQ=255)                                                                                aaaaCGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGc                                                             <  1:679647/133‑1 (MQ=255)                                                                                aaaaCGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGc                                                             >  2:679647/1‑133 (MQ=255)                                                                                  aaCGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATcc                                                    <  1:751870/140‑1 (MQ=255)                                                                                      tGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCg                                                >  1:652228/1‑140 (MQ=255)                                                                                        cTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGaa                                              <  2:652228/140‑1 (MQ=255)                                                                                         tGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAat                                             <  2:483700/140‑1 (MQ=255)                                                                                         tGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAat                                             >  2:992345/1‑140 (MQ=255)                                                                                         tGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAat                                             <  2:1195585/140‑1 (MQ=255)                                                                                         tGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAat                                             >  2:1081412/1‑140 (MQ=255)                                                                                               agagTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCtt                                       >  2:98600/1‑140 (MQ=255)                                                                                                 agTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTaa                                     >  1:313958/1‑140 (MQ=255)                                                                                                 agTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTaa                                     <  2:313958/140‑1 (MQ=255)                                                                                                 agTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTaa                                     <  2:1192882/140‑1 (MQ=255)                                                                                                 agTTATACCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTaa                                     <  1:98600/140‑1 (MQ=255)                                                                                                     ataCCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGc                                 >  2:950809/1‑140 (MQ=255)                                                                                                     ataCCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGc                                 >  1:148951/1‑140 (MQ=255)                                                                                                      taCCGTTTCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCt                                <  1:818685/140‑1 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCtata                             >  1:959901/1‑138 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCtata                             <  2:959901/138‑1 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAc                           >  1:922165/1‑140 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAc                           >  1:886521/1‑140 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAc                           >  2:367615/1‑140 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAc                           <  2:886521/140‑1 (MQ=255)                                                                                                           tttCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAc                           <  2:922165/140‑1 (MQ=255)                                                                                                             tCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCtata                             >  1:371212/1‑136 (MQ=255)                                                                                                             tCCGATGACGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCtata                             <  2:371212/136‑1 (MQ=255)                                                                                                                     cGGCATTACTTACACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGa                 >  1:366015/1‑140 (MQ=255)                                                                                                                        cATTACTTACACCGTGGAATTGCGGGAAGGAATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGtata                    >  1:493560/1‑134 (MQ=255)                                                                                                                        cATTACTTACACCGTGGAATTGCGGGAAGGAATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGtata                    <  2:493560/134‑1 (MQ=255)                                                                                                                            acttacACCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGc          <  2:95559/140‑1 (MQ=255)                                                                                                                                  acCGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAAc    >  1:797229/1‑140 (MQ=255)                                                                                                                                    cGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAata              <  1:481771/128‑1 (MQ=255)                                                                                                                                    cGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAata              >  2:481771/1‑128 (MQ=255)                                                                                                                                    cGTGGAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACgg  >  1:1124813/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGG  >  NC_000913/869546‑869816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑