Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,624	T→C	noncoding (29/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,624	0	T	C	100.0%	101.7 / NA	30	noncoding (29/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

CTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAAT  >  NC_000913/1097496‑1097756                                                                                                                                 |                                                                                                                                     ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGc                                                                                                                           <  1:961757/140‑1 (MQ=255) ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGc                                                                                                                           <  1:32749/140‑1 (MQ=255)  tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGcc                                                                                                                          <  1:940855/140‑1 (MQ=255)         acaggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGa                                                                                                                   <  1:1195785/137‑1 (MQ=255)                tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCa                                                                                                            <  1:803734/140‑1 (MQ=255)                          aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCCTACGTTTCCGTATAAACACCTTTCACGCGCGCCCCTTCCGCCACCCGGGCACCCCCCCAAATTTTc                                                                                                  >  2:505986/1‑140 (MQ=11)                               aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACACGGACACCTCACCAAATTGTCGTTcc                                                                                             <  1:1514003/140‑1 (MQ=255)                                 aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                                                                                           <  1:320375/140‑1 (MQ=255)                                                  tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcg                                                                          <  1:505986/140‑1 (MQ=255)                                                  tACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcg                                                                          <  2:460092/140‑1 (MQ=255)                                                       gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGGACCCCCCCCACCTTTCCGGATACACACCTTTCAGGGGCGCCCCCTCATCCCCCCCGACACCCCACCCAAATTTCGTTTCTGTCTTTCTGGGACGGGGGCTAAt                                                                     >  2:438480/1‑140 (MQ=9)                                                             cttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAggg                                                               <  1:438480/140‑1 (MQ=255)                                                             cttTAAATATGGCGGTGAGGGGGGGATTAGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAggg                                                               <  1:839412/140‑1 (MQ=255)                                                                                          gAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt                                  >  2:326195/1‑140 (MQ=255)                                                                                           aaCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt                                 >  1:364844/1‑140 (MQ=255)                                                                                                        ttGCCGTATACACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATcgcgc                    >  2:259001/1‑140 (MQ=255)                                                                                                              tataCACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTATTTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTAt              >  1:109793/1‑140 (MQ=255)                                                                                                                taCACACTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTc            <  1:713763/140‑1 (MQ=255)                                                                                                                 acacacTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCa           <  2:150284/140‑1 (MQ=255)                                                                                                                     acTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAAc        <  1:946404/139‑1 (MQ=255)                                                                                                                     acTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAAc        >  2:946404/1‑139 (MQ=255)                                                                                                                     acTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACt       >  2:1149144/1‑140 (MQ=255)                                                                                                                     acTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACt       >  2:522240/1‑140 (MQ=255)                                                                                                                      cTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACtt      >  2:1184320/1‑140 (MQ=255)                                                                                                                      cTTTCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACtt      <  2:429902/140‑1 (MQ=255)                                                                                                                       tttCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCaa          <  2:1051352/135‑1 (MQ=255)                                                                                                                       tttCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCaa          >  1:1051352/1‑135 (MQ=255)                                                                                                                        ttCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtca    >  2:51773/1‑140 (MQ=255)                                                                                                                        ttCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtc     >  1:673227/1‑139 (MQ=255)                                                                                                                        ttCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtc     <  2:673227/139‑1 (MQ=255)                                                                                                                         tCCAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtcaa   >  1:1467005/1‑140 (MQ=255)                                                                                                                          ccAGGCGCGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTtcaat  >  2:779962/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     CTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAAT  >  NC_000913/1097496‑1097756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑