Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,388,633	A→G	V60A (GTT→GCT)	tpx ←	lipid hydroperoxide peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,388,633	0	A	G	100.0%	184.5 / NA	55	V60A (GTT→GCT)	tpx	lipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/25);  total (30/25)

GGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGG  >  NC_000913/1388497‑1388768                                                                                                                                         |                                                                                                                                        ggTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAgcac                                                                                                                                      <  2:608833/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     <  1:1041990/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     <  2:536958/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     <  2:1360715/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     <  1:158335/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     <  1:547780/140‑1 (MQ=255)  gTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCAcc                                                                                                                                     >  1:233394/1‑140 (MQ=255)        aCGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATc                                                                                                                               <  2:204380/140‑1 (MQ=255)           ttgttCAGACCTTCTGCGCCACAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAAt                                                                                                                            >  1:1341698/1‑140 (MQ=255)            tgttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATa                                                                                                                           >  2:1296313/1‑140 (MQ=255)             gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATAc                                                                                                                          >  2:885170/1‑140 (MQ=255)             gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATAc                                                                                                                          >  2:1404084/1‑140 (MQ=255)                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAGCTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGaaa                                                                                                                  <  1:517261/137‑1 (MQ=255)                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAGCTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGaaa                                                                                                                  >  2:517261/1‑137 (MQ=255)                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATg                                                                                                               >  2:337572/1‑140 (MQ=255)                           cgccgcAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTc                                                                                                            <  2:587878/140‑1 (MQ=255)                            gccgcAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCa                                                                                                           <  1:1296313/140‑1 (MQ=255)                                      cGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCg                                                                                                 <  1:337572/140‑1 (MQ=255)                                              ggCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCg                                                                                         >  2:873239/1‑140 (MQ=255)                                              ggCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGGTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCg                                                                                         >  1:35516/1‑140 (MQ=255)                                                  aaCGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCaaa                                                                                     <  1:684832/140‑1 (MQ=255)                                                        aGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGAcc                                                                               <  2:233394/140‑1 (MQ=255)                                                             ggCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAggg                                                                          <  1:873239/140‑1 (MQ=255)                                                                         caGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTcagaca                                                              >  1:939965/1‑140 (MQ=255)                                                                            cacaACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGAt                                                           >  1:1031766/1‑140 (MQ=255)                                                                            cacaACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGAt                                                           >  2:818928/1‑140 (MQ=255)                                                                              caACGGTGTTGTCGATCCCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCt                                                         >  2:1067905/1‑140 (MQ=255)                                                                               aaCGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCtt                                                        >  1:1059313/1‑140 (MQ=255)                                                                                 cGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCtttt                                                      >  2:15140/1‑140 (MQ=255)                                                                                 cGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCtttt                                                      >  2:232214/1‑140 (MQ=255)                                                                                   gtgtTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGc                                                    <  2:1105506/140‑1 (MQ=255)                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                 <  2:1205770/140‑1 (MQ=255)                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                 >  2:1315864/1‑140 (MQ=255)                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                 <  1:4138/140‑1 (MQ=255)                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                 >  2:173695/1‑140 (MQ=255)                                                                                        gtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACga                                               >  2:375499/1‑140 (MQ=255)                                                                                         tCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTTGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACgag                                              >  2:735520/1‑140 (MQ=255)                                                                                         tCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACgag                                              >  2:748799/1‑140 (MQ=255)                                                                                           gATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGagag                                            >  1:250816/1‑140 (MQ=255)                                                                                             tctcAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTa                                          <  1:818928/140‑1 (MQ=255)                                                                                                  gTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGt                                     <  2:250816/140‑1 (MQ=255)                                                                                                       cAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTTGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTgcg                                <  1:735520/140‑1 (MQ=255)                                                                                                       cAGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAACTGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTgcg                                <  2:1341698/140‑1 (MQ=255)                                                                                                        aGTTGGTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTGGGGAAAATGTTCCGCACTTCGCGTTGAGCCGCAAACTGACCGAGGGAGACGTCATACAGATGTTTTGCCACGAGGGTAAAAGTCTgcgt                               >  2:1270030/1‑139 (MQ=255)                                                                                                          ttGGTTAAACTTACGTAGTGATGGGGCGCAAGCGCTGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTGCCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCCCGAGAGTAAAAGTCTGCGCtt                             <  2:35516/140‑1 (MQ=255)                                                                                                             gTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGc                          >  1:137488/1‑140 (MQ=255)                                                                                                             gTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGc                          <  1:232214/140‑1 (MQ=255)                                                                                                             gTTAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGc                          <  1:921784/140‑1 (MQ=255)                                                                                                               tAAACTTACGTACTGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAATTCTGCGCTTTGCTa                        <  2:1031766/140‑1 (MQ=255)                                                                                                                            tGATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGa           >  1:22360/1‑140 (MQ=255)                                                                                                                             gATGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGAt          >  1:967398/1‑140 (MQ=255)                                                                                                                              aTGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGc                   >  2:1479722/1‑130 (MQ=255)                                                                                                                              aTGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGc                   <  1:1479722/130‑1 (MQ=255)                                                                                                                              aTGCGGCGCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATg         >  1:173628/1‑140 (MQ=255)                                                                                                                                 cggcgCAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGaa      >  1:1435992/1‑140 (MQ=255)                                                                                                                                   gcgcAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAAtt    >  2:26897/1‑140 (MQ=255)                                                                                                                                    cgcAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGACAGATCTTTTGCCACGAGAGAAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTg   <  1:375499/140‑1 (MQ=255)                                                                                                                                     gcAAGCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTgg  <  1:1056886/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGG  >  NC_000913/1388497‑1388768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑