Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,046	A→G	Y89C (TAC→TGC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,046	0	A	G	100.0%	283.2 / NA	82	Y89C (TAC→TGC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/42);  total (40/42)

TGCCAGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTACCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCGAAGTTCCT  >  NC_000913/1506909‑1507169                                                                                                                                          |                                                                                                                            tGCCAGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGcc                                                                                                                           <  2:641785/140‑1 (MQ=255)     aGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAc                                                                                                                       <  1:778527/140‑1 (MQ=255)     aGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAc                                                                                                                       >  1:759915/1‑140 (MQ=255)     aGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAc                                                                                                                       <  1:450876/140‑1 (MQ=255)     aGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAc                                                                                                                       >  1:940113/1‑140 (MQ=255)     aGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAc                                                                                                                       <  2:456299/140‑1 (MQ=255)      gTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAcc                                                                                                                      >  1:1077312/1‑140 (MQ=255)      gTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGAcc                                                                                                                      >  2:999117/1‑140 (MQ=255)       tAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCg                                                                                                                     >  1:1382508/1‑140 (MQ=255)       tAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCg                                                                                                                     <  2:398973/140‑1 (MQ=255)        aaTAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCgg                                                                                                                    <  1:726478/140‑1 (MQ=255)          tAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGgtg                                                                                                                  >  2:409688/1‑140 (MQ=255)             cTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCg                                                                                                               >  1:827243/1‑140 (MQ=255)               tGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGAt                                                                                                             >  1:890911/1‑140 (MQ=255)                 aaaGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATgc                                                                                                           >  2:409902/1‑140 (MQ=255)                 aaaGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATgc                                                                                                           >  1:698107/1‑140 (MQ=255)                 aaaGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATgc                                                                                                           >  2:284118/1‑140 (MQ=255)                 aaaGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATgc                                                                                                           >  2:279204/1‑140 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      >  1:194542/1‑140 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  1:987411/140‑1 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  2:827243/140‑1 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  2:1382508/140‑1 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  2:1390172/140‑1 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  1:422547/140‑1 (MQ=255)                      tatTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  1:293357/140‑1 (MQ=255)                        ttGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGAtt                                                                                                    >  2:826463/1‑140 (MQ=255)                          gCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGt                                                                                                  <  1:826463/140‑1 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      >  1:1387874/1‑135 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGa                                                                                                      <  2:1387874/135‑1 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGtt                                                                                                 <  2:1296019/140‑1 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGtt                                                                                                 <  2:1010741/140‑1 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGtt                                                                                                 >  1:499384/1‑140 (MQ=255)                           ccGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGtt                                                                                                 >  2:607291/1‑140 (MQ=255)                            cGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTc                                                                                                <  2:299359/140‑1 (MQ=255)                            cGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTc                                                                                                <  1:908202/140‑1 (MQ=255)                            cGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTc                                                                                                <  1:284118/140‑1 (MQ=255)                              aGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGAt                                                                                                     <  1:984709/133‑1 (MQ=255)                              aGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGAt                                                                                                     >  2:984709/1‑133 (MQ=255)                                cTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCa                                                                                               >  2:1269602/1‑137 (MQ=255)                                cTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCa                                                                                               <  1:1269602/137‑1 (MQ=255)                                cTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGa                                                                                            >  1:415257/1‑140 (MQ=255)                                cTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGa                                                                                            >  1:693178/1‑140 (MQ=255)                                         tttGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGAt                                                                                   >  1:750803/1‑140 (MQ=255)                                             cccATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTg                                                                               <  2:946157/140‑1 (MQ=255)                                             cccATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTg                                                                               <  1:999117/140‑1 (MQ=255)                                             cccATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTg                                                                               >  2:54803/1‑140 (MQ=255)                                               cATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGa                                                                             <  2:416347/140‑1 (MQ=255)                                                     tATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGa                                                                       >  2:1275495/1‑140 (MQ=255)                                                       tGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCCGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGata                                                                     >  1:1321932/1‑140 (MQ=255)                                                          tGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTc                                                                  >  2:183280/1‑140 (MQ=255)                                                          tGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCT‑GAACTTGATATTcc                                                                 >  1:442164/1‑140 (MQ=255)                                                           gCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTcc                                                                 <  1:132465/140‑1 (MQ=255)                                                            cAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGGTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTccg                                                                >  2:1366169/1‑140 (MQ=255)                                                            cAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTccg                                                                >  2:457578/1‑140 (MQ=255)                                                             aaaaaTTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTccgc                                                               <  2:415257/140‑1 (MQ=255)                                                              aaaaTTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTccgcc                                                              <  2:759915/140‑1 (MQ=255)                                                                   tttCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTg                                                         <  2:194542/140‑1 (MQ=255)                                                                   tttCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTg                                                         <  2:499384/140‑1 (MQ=255)                                                                        tCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTg                                                    <  1:607291/140‑1 (MQ=255)                                                                          aCGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCa                                                  <  2:1077312/140‑1 (MQ=255)                                                                          aCGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCa                                                  <  1:1366169/140‑1 (MQ=255)                                                                           cGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCT‑GAACTTGATATTCCGCCGATTGAACTGCACg                                                <  2:442164/140‑1 (MQ=255)                                                                                      aTTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGATGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCa                                      >  2:1479206/1‑140 (MQ=255)                                                                                       ttttGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCa                                                  >  1:21608/1‑127 (MQ=255)                                                                                       ttttGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCa                                                  <  2:21608/127‑1 (MQ=255)                                                                                        tttGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGTGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGt                                    <  2:698107/140‑1 (MQ=255)                                                                                        tttGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGt                                    >  2:1010472/1‑140 (MQ=255)                                                                                          tGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGc                                  >  2:1353990/1‑140 (MQ=255)                                                                                            cATGATGATGAGCTGGGACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGa                                >  1:963586/1‑140 (MQ=255)                                                                                            cATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGa                                <  1:457578/140‑1 (MQ=255)                                                                                            cATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGATGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGa                                <  1:1479206/140‑1 (MQ=255)                                                                                               gatgatgaGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACAt                             >  2:1334434/1‑140 (MQ=255)                                                                                                    tgaGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTa                        >  1:533612/1‑140 (MQ=255)                                                                                                           gAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGa                 >  1:198530/1‑140 (MQ=255)                                                                                                           gAACCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGa                 >  2:1457791/1‑140 (MQ=255)                                                                                                             aCCCGCGCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGaaa               <  2:770527/140‑1 (MQ=255)                                                                                                                cgcgCAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAg            >  1:720664/1‑140 (MQ=255)                                                                                                                  cgcAACGGCTGATTCCTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACGGTTGAAAAAGCg          <  2:940113/140‑1 (MQ=255)                                                                                                                  cgcAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCg          <  1:1334434/140‑1 (MQ=255)                                                                                                                  cgcAACGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCg          <  2:890911/140‑1 (MQ=255)                                                                                                                     aaCGGCTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCGAAg       >  2:1403625/1‑140 (MQ=255)                                                                                                                          cTGATTACTGACCTCTGCCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCGAAGTTCct  <  1:1353990/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                            TGCCAGTAATAGCTTGAAAGATATTGCCGAGCTGGTGCCGTTTGCCCATCGTTATGGTGCAAAAATTTTCGTCACGCTTAACACCATTTTGCATGATGATGAGCTGGAACCCGCGCAACGGCTGATTACTGACCTCTACCAGACCGGTGTCGATGCGCTGATTGTTCAGGATATGGGGATTCTGGAACTTGATATTCCGCCGATTGAACTGCACGCCAGTACGCAGTGCGACATTCGTACAGTTGAAAAAGCGAAGTTCCT  >  NC_000913/1506909‑1507169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑