Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,694,161	A→G	Y35H (TAC→CAC)	uidB ←	glucuronide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,694,161	0	A	G	100.0%	180.7 / NA	53	Y35H (TAC→CAC)	uidB	glucuronide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GGCGGAATTTTCCCCAGCGGGTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTAGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCTGCTGCGGTTTTTCACCG  >  NC_000913/1694026‑1694293                                                                                                                                        |                                                                                                                                     ggCGGAATTTTCCCCAGCGGGTATTCACACTGCCCACCACTCGTCCGGAAAAGACGGCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTaa                                                                                                                                  <  2:1508696/140‑1 (MQ=255)    ggAATTTTCCCCAGCGGGTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTc                                                                                                                               <  2:1258415/140‑1 (MQ=255)           tCCCCAGCGGGTATTCACACTGTCCACCACTCGTCCGGCAAGGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGa                                                                                                                        <  2:443362/140‑1 (MQ=255)               cAGCGGGTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAagag                                                                                                                    <  1:775109/140‑1 (MQ=255)                  cGGGTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAgcgc                                                                                                                 >  1:864480/1‑140 (MQ=255)                     gTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGcccc                                                                                                              <  2:627944/140‑1 (MQ=255)                               tGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaag                                                                                                    >  2:9296/1‑140 (MQ=255)                                gTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaagg                                                                                                   >  2:520405/1‑140 (MQ=255)                                gTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaagg                                                                                                   >  1:246282/1‑140 (MQ=255)                                gTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaagg                                                                                                   >  1:1101928/1‑140 (MQ=255)                                 tCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaaggc                                                                                                  >  1:152991/1‑140 (MQ=255)                                 tCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaaggc                                                                                                  <  1:9296/140‑1 (MQ=255)                                  ccaccaCTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaaggcg                                                                                                 >  2:387908/1‑140 (MQ=255)                                      cacTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaaggcg                                                                                                 <  1:1003690/136‑1 (MQ=255)                                      cacTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaaggcg                                                                                                 >  2:1003690/1‑136 (MQ=255)                                        cTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTa                                                                                           <  2:695846/140‑1 (MQ=255)                                         tCGTCCGGCAAAGGCGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaagg                                                                                                   <  1:769242/131‑1 (MQ=255)                                         tCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTgcgaagg                                                                                                   >  2:769242/1‑131 (MQ=255)                                           gTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTg                                                                                        >  1:720130/1‑140 (MQ=255)                                           gTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTg                                                                                        >  1:939765/1‑140 (MQ=255)                                           gTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTg                                                                                        >  2:40789/1‑140 (MQ=255)                                           gTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTg                                                                                        >  1:879804/1‑140 (MQ=255)                                             ccGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGc                                                                                      <  2:246282/140‑1 (MQ=255)                                                     gACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCAc                                                                              <  1:40789/140‑1 (MQ=255)                                                                  gCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGcc                                                                 <  2:879804/140‑1 (MQ=255)                                                                      cGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCcgacg                                                             >  2:1235164/1‑140 (MQ=255)                                                                          tACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGTGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATgg                                                         <  1:698952/140‑1 (MQ=255)                                                                            cccGCACCAGTAACAGCATGGTGCCCGCCGCAGCGACACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTg                                                       >  1:317882/1‑140 (MQ=255)                                                                              cGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTgcg                                                     >  2:100490/1‑140 (MQ=255)                                                                               gCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTgcgc                                                    <  1:520405/140‑1 (MQ=255)                                                                                    aGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGga                                               <  1:1235164/140‑1 (MQ=255)                                                                                             aTGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGgaga                                             <  1:1361343/133‑1 (MQ=255)                                                                                             aTGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGgaga                                             >  2:1361343/1‑133 (MQ=255)                                                                                             aTGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGgaga                                             <  1:713993/133‑1 (MQ=255)                                                                                             aTGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGgaga                                             >  2:713993/1‑133 (MQ=255)                                                                                               ggTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGttgttgtt                                    >  1:651385/1‑138 (MQ=255)                                                                                               ggTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAt                                    >  1:762784/1‑140 (MQ=255)                                                                                                gTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   >  2:1213831/1‑140 (MQ=255)                                                                                                gTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   >  2:891493/1‑140 (MQ=255)                                                                                                 tGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   >  1:1110037/1‑139 (MQ=255)                                                                                                 tGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   <  2:1110037/139‑1 (MQ=255)                                                                                                    ccgccgCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   >  2:710364/1‑136 (MQ=255)                                                                                                    ccgccgCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   <  1:1061536/136‑1 (MQ=255)                                                                                                    ccgccgCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   >  2:1061536/1‑136 (MQ=255)                                                                                                    ccgccgCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGAtt                                   <  1:710364/136‑1 (MQ=255)                                                                                                    ccgccgCAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCAtt                               <  2:152991/140‑1 (MQ=255)                                                                                                       ccgcAGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGtt                            <  1:1213831/140‑1 (MQ=255)                                                                                                           aGCGGCACCGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGcc                        >  1:990441/1‑140 (MQ=255)                                                                                                                   cGACGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCctgct                <  1:891493/140‑1 (MQ=255)                                                                                                                     aCGCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCtgctgc              <  2:1157156/140‑1 (MQ=255)                                                                                                                       gCCAGCGACGTCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCTGCTGCgg            <  2:864480/140‑1 (MQ=255)                                                                                                                                 tCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCTGCTGCGGTTTTTCACCg  <  2:1101928/140‑1 (MQ=255)                                                                                                                                 tCGGTGTGGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCTGCTGCGGTTTTTCACCg  <  1:1323591/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GGCGGAATTTTCCCCAGCGGGTATTCACACTGTCCACCACTCGTCCGGCAAAGACGTCGGCGAAGGCATCGAATACCCGCACCAGTAACAGCATGGTGCCCGCCGCAGCGGCACCGACGCCAGCGACGTCGGTGTAGTAACTCAACAGGAAGAGCGCCCCCATTGCGAAGGCGAAGTTATTGGCGACGTCACCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTGATTCATTGTTTGCCTCCCTGCTGCGGTTTTTCACCG  >  NC_000913/1694026‑1694293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑