Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,426	T→C	V194V (GTT→GTC)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,426	0	T	C	100.0%	191.2 / NA	56	V194V (GTT→GTC)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/27);  total (29/27)

TCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCTG  >  NC_000913/1886291‑1886555                                                                                                                                        |                                                                                                                                  tCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCtata                                                                                                                               <  1:944893/140‑1 (MQ=255)    aGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCt                                                                                                                            <  2:1429956/140‑1 (MQ=255)            gcgcATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGcccc                                                                                                                    >  2:1236634/1‑140 (MQ=255)             cgcATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCg                                                                                                                   >  1:953922/1‑140 (MQ=255)             cgcATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCg                                                                                                                   >  2:1006454/1‑140 (MQ=255)                aTTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAgg                                                                                                                >  1:332293/1‑140 (MQ=255)                 tttCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGgc                                                                                                               <  1:374039/140‑1 (MQ=255)                           aGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGaa                                                                                                     <  2:1412609/140‑1 (MQ=255)                            gCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAg                                                                                                    <  2:581764/140‑1 (MQ=255)                                     gCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTaagaa                                                                                           >  1:553409/1‑140 (MQ=255)                                      cATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGc                                                                                                   <  1:1514233/131‑1 (MQ=255)                                      cATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGc                                                                                                   >  2:1514233/1‑131 (MQ=255)                                      cATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTaagaag                                                                                          >  2:1134083/1‑140 (MQ=255)                                       aTCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGt                                                                                         >  1:447980/1‑140 (MQ=255)                                           gCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAg                                                                                     >  2:259124/1‑140 (MQ=255)                                            cAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGc                                                                                    <  2:1239778/140‑1 (MQ=255)                                              ggCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTg                                                                                  <  1:468358/140‑1 (MQ=255)                                                   agaAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGt                                                                             <  1:1134083/140‑1 (MQ=255)                                                   agaAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGt                                                                             >  1:77482/1‑140 (MQ=255)                                                         tCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGAc                                                                       >  2:345365/1‑140 (MQ=255)                                                                  atcatTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCgccgcc                                                              >  1:690477/1‑140 (MQ=255)                                                                   tcattcatCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGccgccg                                                             <  1:1059148/140‑1 (MQ=255)                                                                   tcattcatCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGccgccg                                                             >  1:340302/1‑140 (MQ=255)                                                                       tcatcaACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATAc                                                         >  1:184953/1‑140 (MQ=255)                                                                                ggAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACtt                                                >  2:697980/1‑140 (MQ=255)                                                                                 gAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACttt                                               >  1:624289/1‑140 (MQ=255)                                                                                 gAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACttt                                               >  1:649170/1‑140 (MQ=255)                                                                                 gAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACttt                                               >  1:1126410/1‑140 (MQ=255)                                                                                       gACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCaa                                         <  2:1512075/140‑1 (MQ=255)                                                                                        aCTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAAt                                        >  2:407579/1‑140 (MQ=255)                                                                                        aCTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAAt                                        >  1:1240618/1‑140 (MQ=255)                                                                                           ggAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTg                                     <  1:345365/140‑1 (MQ=255)                                                                                            gAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGc                                    >  1:1079680/1‑140 (MQ=255)                                                                                                   cgcgATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCg                               >  1:58623/1‑138 (MQ=255)                                                                                                   cgcgATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCg                               <  2:58623/138‑1 (MQ=255)                                                                                                   cgcgATTATTGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGaa                             >  1:402965/1‑140 (MQ=255)                                                                                                       attattGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCGCTTTGAGCAATTTGCTATCGAAGACa                         >  1:1071753/1‑140 (MQ=255)                                                                                                       attattGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACa                         >  1:1409550/1‑140 (MQ=255)                                                                                                         tattGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACaaa                       <  2:1126410/140‑1 (MQ=255)                                                                                                           ttGGCGGATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAAc                     <  2:649170/140‑1 (MQ=255)                                                                                                                ggATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCg                <  1:1491006/140‑1 (MQ=255)                                                                                                                ggATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCg                <  1:407579/140‑1 (MQ=255)                                                                                                                ggATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCg                <  2:553409/140‑1 (MQ=255)                                                                                                                cgATATCGAACCTGCCACACACGTATATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGGGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCg                <  2:1071753/139‑1 (MQ=255)                                                                                                                 gATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGa               <  2:184953/140‑1 (MQ=255)                                                                                                                 gATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGa               <  2:690477/140‑1 (MQ=255)                                                                                                                 gATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGa               <  2:332293/140‑1 (MQ=255)                                                                                                                 gATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGa               <  2:1079680/140‑1 (MQ=255)                                                                                                                 gATATCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGa               <  1:1006454/140‑1 (MQ=255)                                                                                                                  atatCGAACCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGAt              >  1:711037/1‑140 (MQ=255)                                                                                                                        aaCCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGc            <  2:242906/136‑1 (MQ=255)                                                                                                                        aaCCTGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGc            >  1:242906/1‑136 (MQ=255)                                                                                                                            tGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAGGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTAccc    <  2:1409550/140‑1 (MQ=255)                                                                                                                            tGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTAccc    <  2:77482/140‑1 (MQ=255)                                                                                                                            tGGCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTAccc    <  2:402965/140‑1 (MQ=255)                                                                                                                             ggCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCt   >  2:263876/1‑140 (MQ=255)                                                                                                                             ggCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCt   >  1:1432883/1‑140 (MQ=255)                                                                                                                              gCACACACGTCTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCTg  >  1:548276/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  TCCAGAAAGTTGCGCATTTCGTGATGAGCTAGTCCAGCATCCGCAGGCTGAGAAAGTCCATTTGCATCATTCATCAACCGGAACACGACTGGAATTAGCGCGATTATTGGCGGATATCGAACCTGGCACACACGTTTATACCTGTGGCCCCGAGGCGCTAATTGAAGCGGTAAGAAGTGAAGCTGCGCGTCTGGACATCGCCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCTG  >  NC_000913/1886291‑1886555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑