Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,938,424	A→G	D259G (GAC→GGC)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,938,424	0	A	G	100.0%	299.0 / NA	85	D259G (GAC→GGC)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/47);  total (38/47)

CAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGT  >  NC_000913/1938289‑1938559                                                                                                                                        |                                                                                                                                        cAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCg                                                                                                                                     >  2:260172/1‑140 (MQ=255)    gATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGaa                                                                                                                                  <  1:1065038/140‑1 (MQ=255)    gATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGaa                                                                                                                                  <  1:1343275/140‑1 (MQ=255)      tgtgATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACt                                                                                                                                >  2:453848/1‑140 (MQ=255)         gATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCgg                                                                                                                                    >  2:1255827/1‑133 (MQ=255)         gATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCgg                                                                                                                                    <  1:1255827/133‑1 (MQ=255)         gATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGt                                                                                                                             >  2:891179/1‑140 (MQ=255)         gATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGt                                                                                                                             <  1:891179/140‑1 (MQ=255)          aTGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTc                                                                                                                            <  1:453848/140‑1 (MQ=255)              gAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCa                                                                                                                        >  1:58066/1‑140 (MQ=255)               aaaaTTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCAt                                                                                                                       <  1:1002857/140‑1 (MQ=255)               aaaaTTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCACCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCAt                                                                                                                       >  2:542682/1‑140 (MQ=255)                  aTTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCa                                                                                                                    <  2:213327/140‑1 (MQ=255)                     gttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGaa                                                                                                                 <  1:977053/140‑1 (MQ=255)                      ttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGaaa                                                                                                                >  1:495418/1‑140 (MQ=255)                      ttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGaaa                                                                                                                >  2:787836/1‑140 (MQ=255)                      ttgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGaaa                                                                                                                >  1:1085180/1‑140 (MQ=255)                       tgCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAg                                                                                                               >  1:1259210/1‑140 (MQ=255)                         ccAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCg                                                                                                             <  2:58066/140‑1 (MQ=255)                           aaGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGtt                                                                                                           <  1:565689/140‑1 (MQ=255)                            aGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTg                                                                                                          >  1:1374543/1‑140 (MQ=255)                                 gAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCcg                                                                                                     <  2:648403/140‑1 (MQ=255)                                      tGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTg                                                                                                <  2:1259210/140‑1 (MQ=255)                                        cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                                              >  2:412398/1‑140 (MQ=255)                                        cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                                              >  2:1087505/1‑140 (MQ=255)                                        cGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcg                                                                                              >  1:1262392/1‑140 (MQ=255)                                         ggAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcgc                                                                                             <  2:186392/140‑1 (MQ=255)                                         ggAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcgc                                                                                             <  2:562331/140‑1 (MQ=255)                                         ggAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCACCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTgcgc                                                                                             <  1:542682/140‑1 (MQ=255)                                                gTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCt                                                                                      <  2:1092671/140‑1 (MQ=255)                                                 tttGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTa                                                                                     >  2:196938/1‑140 (MQ=255)                                                      aGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCg                                                                                <  1:1052056/140‑1 (MQ=255)                                                        ccAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAg                                                                              <  1:196938/140‑1 (MQ=255)                                                        ccAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAg                                                                              >  1:69663/1‑140 (MQ=255)                                                               gCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAAt                                                                       <  2:565798/140‑1 (MQ=255)                                                                  aTGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCAc                                                                    >  2:1364542/1‑140 (MQ=255)                                                                   tGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACg                                                                   <  1:1364542/140‑1 (MQ=255)                                                                    ggATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACgg                                                                  <  2:1435659/140‑1 (MQ=255)                                                                      aTGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCg                                                                >  2:863552/1‑140 (MQ=255)                                                                        gACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAc                                                              <  1:1156754/140‑1 (MQ=255)                                                                          catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                            >  2:409219/1‑140 (MQ=255)                                                                          catcatCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGAccc                                                            >  2:1486060/1‑140 (MQ=255)                                                                            tcatcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAg                                                          <  1:1486060/140‑1 (MQ=255)                                                                               tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                                       >  1:478964/1‑140 (MQ=255)                                                                               tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                                       >  1:234005/1‑140 (MQ=255)                                                                               tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                                       >  1:902365/1‑140 (MQ=255)                                                                               tcCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAgatg                                                       >  1:344387/1‑140 (MQ=255)                                                                                  tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                    <  1:1506695/140‑1 (MQ=255)                                                                                  tCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                    <  1:260172/140‑1 (MQ=255)                                                                                    gCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGa                                                  >  1:1326996/1‑140 (MQ=255)                                                                                     ccTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAg                                                 <  2:335037/140‑1 (MQ=255)                                                                                         tGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                    >  2:541068/1‑133 (MQ=255)                                                                                         tGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGatgatg                                                    <  1:541068/133‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  1:263596/140‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  2:1326996/140‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  2:1374543/140‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  1:412398/140‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  2:234005/140‑1 (MQ=255)                                                                                          gACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  1:787836/140‑1 (MQ=255)                                                                                          acagTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  2:549336/137‑1 (MQ=255)                                                                                                gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            <  1:669973/134‑1 (MQ=255)                                                                                                gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAAt                                            >  2:669973/1‑134 (MQ=255)                                                                                                gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                      >  2:409605/1‑140 (MQ=255)                                                                                                gTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTAc                                      >  1:463060/1‑140 (MQ=255)                                                                                                     ggTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                 <  1:863552/140‑1 (MQ=255)                                                                                                       ttGCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCg                               <  1:238005/140‑1 (MQ=255)                                                                                                         gCACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGAt                             >  2:1155652/1‑140 (MQ=255)                                                                                                          cACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                            >  1:778887/1‑140 (MQ=255)                                                                                                          cACGTGGCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATg                            >  2:181852/1‑140 (MQ=255)                                                                                                               ggCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc                       >  1:56744/1‑140 (MQ=255)                                                                                                               ggCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc                       <  2:344387/140‑1 (MQ=255)                                                                                                               ggCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAc                       >  1:761620/1‑140 (MQ=255)                                                                                                                gCGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGAcg                      >  1:149337/1‑140 (MQ=255)                                                                                                                 cGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                 <  1:1210688/128‑1 (MQ=255)                                                                                                                 cGACCTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAAcc                                 >  2:1210688/1‑128 (MQ=255)                                                                                                                    ccTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTg                  <  2:495418/140‑1 (MQ=255)                                                                                                                    ccTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTg                  <  2:1085180/140‑1 (MQ=255)                                                                                                                    ccTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTg                  >  2:621512/1‑140 (MQ=255)                                                                                                                     cTCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGc                 >  1:1230349/1‑140 (MQ=255)                                                                                                                      tCGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCa                <  1:1087505/140‑1 (MQ=255)                                                                                                                       cGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAg               <  2:149337/140‑1 (MQ=255)                                                                                                                       cGGTGTGGAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAg               <  2:1262392/140‑1 (MQ=255)                                                                                                                             ggAAATTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCa         <  2:463060/140‑1 (MQ=255)                                                                                                                                aaTTGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATgg      <  2:761620/140‑1 (MQ=255)                                                                                                                                  ttGGCGGCCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGAc    <  1:971989/140‑1 (MQ=255)                                                                                                                                    ggcggcCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGt  >  1:1284640/1‑140 (MQ=255)                                                                                                                                    ggcggcCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGt  >  2:1242238/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTTTGCAGCCAGGATGCAATGGATGACATCATCCTCGCCTCTGACGTGGTAATGGTTGCACGTGGCGACCTCGGTGTGGAAATTGGCGACCCGGAACTGGTCGGCATTCAGAAAGCGTTGATCCGTCGTGCGCGTCAGCTAAACCGAGCGGTAATCACGGCGACCCAGATGATGGAGTCAATGATTACTAACCCGATGCCGACGCGTGCAGAAGTCATGGACGT  >  NC_000913/1938289‑1938559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑