Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,063	A→G	N934S (AAC→AGC)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,063	0	A	G	100.0%	310.6 / NA	89	N934S (AAC→AGC)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/40);  total (49/40)

TTCACCCGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGC  >  NC_000913/2157928‑2158198                                                                                                                                        |                                                                                                                                        ttCACCCGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGcc                                                                                                                                     <  1:1125926/140‑1 (MQ=255)     cccGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCcatca                                                                                                                                 >  2:66702/1‑140 (MQ=255)      ccGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCcatcat                                                                                                                                >  2:562130/1‑140 (MQ=255)      ccGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCcatcat                                                                                                                                >  1:1506368/1‑140 (MQ=255)      ccGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCcatcat                                                                                                                                >  1:932491/1‑140 (MQ=255)         aTCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCatgat                                                                                                                             >  2:961953/1‑140 (MQ=255)            aCCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATgatgat                                                                                                                          <  1:66702/140‑1 (MQ=255)                ttCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCg                                                                                                                                       >  2:535792/1‑123 (MQ=255)                ttCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGAc                                                                                                                      <  1:752325/140‑1 (MQ=255)                ttCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGAc                                                                                                                      >  2:1003853/1‑140 (MQ=255)                ttCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGAc                                                                                                                      >  1:887066/1‑140 (MQ=255)                    ctcGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcg                                                                                                                  >  1:41828/1‑140 (MQ=255)                    ctcGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcg                                                                                                                  >  1:709348/1‑140 (MQ=255)                     tcGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgc                                                                                                                 >  2:294091/1‑140 (MQ=255)                     tcGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgc                                                                                                                 <  2:1231119/140‑1 (MQ=255)                     tcGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgc                                                                                                                 <  2:978815/140‑1 (MQ=255)                      cGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgcg                                                                                                                <  2:932491/140‑1 (MQ=255)                        aCGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgcg                                                                                                                <  1:890873/138‑1 (MQ=255)                        aCGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgcg                                                                                                                >  2:890873/1‑138 (MQ=255)                        aCGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgct                                                                                                              >  1:339243/1‑140 (MQ=255)                         cGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctg                                                                                                             <  2:887066/140‑1 (MQ=255)                           cTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgc                                                                                                                 <  2:787394/134‑1 (MQ=255)                           cTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTcgc                                                                                                                 >  1:787394/1‑134 (MQ=255)                           cTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctggc                                                                                                           <  2:1094687/140‑1 (MQ=255)                             aCCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctggctg                                                                                                         <  2:1506368/140‑1 (MQ=255)                             aCCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctggctg                                                                                                         <  1:562130/140‑1 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgct                                                                                                              <  1:1386911/129‑1 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgct                                                                                                              >  2:1386911/1‑129 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAgc                                                                                                   >  1:286921/1‑140 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAgc                                                                                                   >  1:1225203/1‑140 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAgc                                                                                                   >  1:656767/1‑140 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAgc                                                                                                   >  2:1209017/1‑140 (MQ=255)                                   cGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAgc                                                                                                   >  1:55406/1‑140 (MQ=255)                                       ggggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGa                                                                                               <  2:386342/140‑1 (MQ=255)                                        gggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              >  1:188717/1‑140 (MQ=255)                                        gggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              <  2:1225203/140‑1 (MQ=255)                                        gggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              >  1:590143/1‑140 (MQ=255)                                        gggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              <  1:1209017/140‑1 (MQ=255)                                        gggTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              >  1:255687/1‑140 (MQ=255)                                          gTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCa                                                                                            >  1:217011/1‑140 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGGGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCa                                                                                            <  1:1403527/137‑1 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGGGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCa                                                                                            >  2:1403527/1‑137 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctggctg                                                                                                         <  2:664596/124‑1 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCgctggctg                                                                                                         >  1:664596/1‑124 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              >  1:600950/1‑135 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAg                                                                                              <  2:600950/135‑1 (MQ=255)                                             ggCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAgg                                                                                         >  1:1014325/1‑140 (MQ=255)                                                    tgctgGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTcg                                                                                  <  1:1003853/140‑1 (MQ=255)                                                    tgctgGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTcg                                                                                  >  1:481927/1‑140 (MQ=255)                                                        ggCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTcgccgc                                                                              >  2:683538/1‑140 (MQ=255)                                                         gCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCcgcg                                                                             <  2:286921/140‑1 (MQ=255)                                                         gCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCcgcg                                                                             <  1:294091/140‑1 (MQ=255)                                                            ttGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGa                                                                          <  1:1278325/140‑1 (MQ=255)                                                            ttGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGa                                                                          <  2:55406/140‑1 (MQ=255)                                                              gCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAgg                                                                        >  1:477373/1‑140 (MQ=255)                                                              gCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAgg                                                                        >  2:1242440/1‑140 (MQ=255)                                                              gCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAgg                                                                        <  2:853666/140‑1 (MQ=255)                                                               cTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGc                                                                       <  2:41828/140‑1 (MQ=255)                                                                tGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCa                                                                      <  2:709348/140‑1 (MQ=255)                                                                 gATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCaa                                                                     <  2:1035801/140‑1 (MQ=255)                                                                 gATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCaa                                                                     <  2:656767/140‑1 (MQ=255)                                                                   ttGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATc                                                                   >  2:1209662/1‑140 (MQ=255)                                                                      cTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTAc                                                                <  1:1209662/140‑1 (MQ=255)                                                                                aCTGGATGTGATTGCGATTATCGTCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTc                                                      >  2:700938/1‑140 (MQ=255)                                                                                aCTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTc                                                      >  2:315606/1‑140 (MQ=255)                                                                                 cTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCt                                                     >  1:1326354/1‑140 (MQ=255)                                                                                    gATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGtt                                                  >  2:1458073/1‑140 (MQ=255)                                                                                    gATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGtt                                                  <  1:1458073/140‑1 (MQ=255)                                                                                          aTTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGttt                                            <  2:1014325/140‑1 (MQ=255)                                                                                              cGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGt                                        >  2:239697/1‑140 (MQ=255)                                                                                              cGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGt                                        >  2:481526/1‑140 (MQ=255)                                                                                               gATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTc                                       <  1:1242440/140‑1 (MQ=255)                                                                                                aTTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTcc                                      >  2:888750/1‑140 (MQ=255)                                                                                                aTTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTcc                                      >  1:1426930/1‑140 (MQ=255)                                                                                                  tATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGa                                    <  2:481927/140‑1 (MQ=255)                                                                                                     cGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATcc                                 >  1:249572/1‑140 (MQ=255)                                                                                                            attTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGAc                          <  1:239697/140‑1 (MQ=255)                                                                                                            attTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGAc                          <  2:255687/140‑1 (MQ=255)                                                                                                            attTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGAc                          <  2:1326354/140‑1 (MQ=255)                                                                                                            attTTGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGAc                          <  1:481526/140‑1 (MQ=255)                                                                                                             ttttGCTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGAcc                         >  1:47509/1‑140 (MQ=255)                                                                                                                  cTGATCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCActct                    >  1:1191554/1‑140 (MQ=255)                                                                                                                     aTCGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTggc                 >  1:840676/1‑140 (MQ=255)                                                                                                                       cGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTggcgg               >  2:35391/1‑140 (MQ=255)                                                                                                                       cGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTgg                  <  1:842205/137‑1 (MQ=255)                                                                                                                       cGGTATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTgg                  >  2:842205/1‑137 (MQ=255)                                                                                                                          tATCGTGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGctc            >  1:1333727/1‑140 (MQ=255)                                                                                                                               tGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGct             <  2:199280/134‑1 (MQ=255)                                                                                                                               tGAAGAAGAGCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGct             >  1:199280/1‑134 (MQ=255)                                                                                                                                 aagaagagCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTgg     >  1:270309/1‑140 (MQ=255)                                                                                                                                  agaagagCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGgc    >  1:741544/1‑140 (MQ=255)                                                                                                                                    aagagCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGgcgc  >  1:168865/1‑140 (MQ=255)                                                                                                                                    aagagCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGgcgc  >  1:309774/1‑140 (MQ=255)                                                                                                                                    aagagCGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGgcgc  >  2:746496/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCACCCGATCACCATTCTCTCGACGCTACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGC  >  NC_000913/2157928‑2158198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑