Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,445,924	G→C	T154R (ACG→AGG)	mepA ←	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,445,924	0	G	C	100.0%	252.0 / NA	73	T154R (ACG→AGG)	mepA	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (41/32);  total (41/32)

TTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCTGGTGGCTGGCA  >  NC_000913/2445788‑2446055                                                                                                                                         |                                                                                                                                    tttGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTgg                                                                                                                                  <  1:553697/140‑1 (MQ=255)   tGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGgag                                                                                                                                >  1:416044/1‑140 (MQ=255)   tGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGgag                                                                                                                                >  1:844032/1‑140 (MQ=255)   tGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGgag                                                                                                                                >  2:112220/1‑140 (MQ=255)     cgcAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGAc                                                                                                                              <  1:197395/140‑1 (MQ=255)        aaCCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAAc                                                                                                                           <  2:757535/140‑1 (MQ=255)             gTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGtt                                                                                                                      >  1:296766/1‑140 (MQ=255)             gTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGAAGACAACGTGtt                                                                                                                      >  1:1378613/1‑140 (MQ=255)               cgcgATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTa                                                                                                                    >  1:996919/1‑140 (MQ=255)                        tGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCgg                                                                                                           >  1:1102694/1‑140 (MQ=255)                              cATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACt                                                                                                     <  2:1102694/140‑1 (MQ=255)                              cATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACt                                                                                                     >  1:676006/1‑140 (MQ=255)                              cATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACt                                                                                                     >  2:1269191/1‑140 (MQ=255)                              cATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACt                                                                                                     <  1:112220/140‑1 (MQ=255)                                 cAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAg                                                                                                  <  2:676006/140‑1 (MQ=255)                                    ggCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTcc                                                                                               >  1:1466279/1‑140 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCggg                                                                                                          <  1:365891/124‑1 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCggg                                                                                                          >  2:365891/1‑124 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTc                                                                                                <  2:943476/134‑1 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTc                                                                                                >  1:943476/1‑134 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGc                                                                                          >  2:724978/1‑140 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGc                                                                                          >  2:397445/1‑140 (MQ=255)                                         aGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGc                                                                                          >  1:1345211/1‑140 (MQ=255)                                            tgttgttTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTg                                                                                       <  2:52138/140‑1 (MQ=255)                                              ttgttTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCg                                                                                     <  2:1176432/140‑1 (MQ=255)                                              ttgttTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCg                                                                                     <  1:636153/140‑1 (MQ=255)                                                   tAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGgcgc                                                                                >  2:1455872/1‑140 (MQ=255)                                                     aTCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCaa                                                                              >  1:956744/1‑140 (MQ=255)                                                           ggATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTg                                                                        <  2:1466279/140‑1 (MQ=255)                                                           ggATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTg                                                                        <  1:346600/140‑1 (MQ=255)                                                                    aaaaTGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGt                                                               <  1:1455872/140‑1 (MQ=255)                                                                      aaTGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTcc                                                             >  2:1114080/1‑140 (MQ=255)                                                                       aTGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCa                                                            >  1:324418/1‑140 (MQ=255)                                                                            cgTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAg                                                       >  2:691087/1‑140 (MQ=255)                                                                            cgTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAg                                                       >  1:447969/1‑140 (MQ=255)                                                                            cgTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAg                                                       >  1:389968/1‑140 (MQ=255)                                                                            cgTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAg                                                       >  1:393539/1‑140 (MQ=255)                                                                                   tCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCgg                                                <  2:844032/140‑1 (MQ=255)                                                                                   tCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCgg                                                <  1:982659/140‑1 (MQ=255)                                                                                     tttGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGa                                                        <  1:559103/130‑1 (MQ=255)                                                                                     tttGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGa                                                        >  2:559103/1‑130 (MQ=255)                                                                                      ttGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAg                                             <  1:397445/140‑1 (MQ=255)                                                                                       tGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGt                                            <  2:324418/140‑1 (MQ=255)                                                                                         tCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTg                                          <  2:393539/140‑1 (MQ=255)                                                                                         tCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTg                                          <  1:724978/140‑1 (MQ=255)                                                                                         tCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTg                                          >  2:504/1‑140 (MQ=255)                                                                                              ggcggcGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGaa                                     >  1:881143/1‑140 (MQ=255)                                                                                              ggcggcGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGaa                                     >  1:252634/1‑140 (MQ=255)                                                                                              ggcggcGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGaa                                     >  1:376704/1‑140 (MQ=255)                                                                                                cggcgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGt                                            <  1:756701/131‑1 (MQ=255)                                                                                                cggcgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGt                                            >  2:756701/1‑131 (MQ=255)                                                                                                cggcgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCGGAAAg                                   <  2:1345211/140‑1 (MQ=255)                                                                                                 ggcgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGa                                  <  2:996919/140‑1 (MQ=255)                                                                                                 ggcgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGa                                  <  1:691087/140‑1 (MQ=255)                                                                                                   cgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGata                                >  1:729750/1‑140 (MQ=255)                                                                                                   cgAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGata                                >  1:31808/1‑140 (MQ=255)                                                                                                      gTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCg                             <  1:506525/140‑1 (MQ=255)                                                                                                         tGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACa                          <  1:1269191/140‑1 (MQ=255)                                                                                                         tGATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACa                          >  1:13220/1‑140 (MQ=255)                                                                                                          gATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACAt                         <  2:296766/140‑1 (MQ=255)                                                                                                          gATCAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACAt                         >  1:153818/1‑140 (MQ=255)                                                                                                             cAAGCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCa                      <  1:1114080/140‑1 (MQ=255)                                                                                                                gCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTc                   >  1:1138263/1‑140 (MQ=255)                                                                                                                gCTGAAAATTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTc                   >  1:44217/1‑140 (MQ=255)                                                                                                                     aaaTTTCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTc              <  2:416044/140‑1 (MQ=255)                                                                                                                        tttCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCtgg           <  2:1400674/140‑1 (MQ=255)                                                                                                                          tCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCtggtg         >  1:1057277/1‑140 (MQ=255)                                                                                                                          tCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCtggtg         >  1:953815/1‑140 (MQ=255)                                                                                                                          tCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCt             >  2:865887/1‑136 (MQ=255)                                                                                                                          tCTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCt             <  1:865887/136‑1 (MQ=255)                                                                                                                           cTGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCtggtgg        >  1:1471767/1‑140 (MQ=255)                                                                                                                            tGGCTTCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCTGGtggc       >  1:488725/1‑140 (MQ=255)                                                                                                                                 tCCACAGCCTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCTGGTGGCTGGCa  >  1:75384/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TTTGCGCAACCAGTCGCGATCGGTGCCCGCATCAAGGCAAAGTTGTTGTTTAATCGCCGGATTAACAAAAATGCGCGTGACGTCTTTGTCCTGGGCGGCGAGTTTGATCAAGCTGAAAATTTCTGGCTTCCACAGCGTGGAGACAACGTGTTTACCGTCGCGGGAAACTAAGTCCAGTGCTTGCGGGCGCAAGAGCTGCGCGGAGGTCCAGCGAGTTTTCGGCAGTTGCAGAAAGATATCGACATCCAGTCCGGTCTGGTGGCTGGCA  >  NC_000913/2445788‑2446055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑