Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,847	C→T	intergenic (+282/‑74)	lpxP → / → ypdK	palmitoleoyl acyltransferase/putative membrane protein YpdK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,847	0	C	T	100.0%	298.8 / NA	84	intergenic (+282/‑74)	lpxP/ypdK	palmitoleoyl acyltransferase/putative membrane protein YpdK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/50);  total (34/50)

AGTTACTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGCCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCC  >  NC_000913/2496710‑2496981                                                                                                                                          |                                                                                                                                       aGTTACTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGtcc                                                                                                                                      <  2:210009/140‑1 (MQ=255)     aCTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtg                                                                                                                                  >  2:227095/1‑140 (MQ=255)     aCTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtg                                                                                                                                  >  1:73029/1‑140 (MQ=255)      cTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGtcc                                                                                                                                      >  2:1303203/1‑135 (MQ=255)      cTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtgt                                                                                                                                 >  2:1506314/1‑140 (MQ=255)      cTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtgt                                                                                                                                 >  2:1162893/1‑140 (MQ=255)      cTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtgt                                                                                                                                 >  2:360586/1‑140 (MQ=255)      cTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGgtgt                                                                                                                                 >  2:598232/1‑140 (MQ=255)       tAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTc                                                                                                                                >  1:1448750/1‑140 (MQ=255)        aaaaGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCa                                                                                                                               <  1:227095/140‑1 (MQ=255)         aaaGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCaa                                                                                                                              <  2:1106024/140‑1 (MQ=255)          aaGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAAc                                                                                                                             <  1:368845/140‑1 (MQ=255)           aGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTGTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGG‑‑GATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCa                                                                                                                          <  2:940879/140‑1 (MQ=255)            gTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTc                                                                                                                           <  2:12864/140‑1 (MQ=255)            gTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTc                                                                                                                           <  2:179161/140‑1 (MQ=255)            gTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTc                                                                                                                           <  1:598232/140‑1 (MQ=255)             tAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCa                                                                                                                          <  1:360586/140‑1 (MQ=255)             tAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCa                                                                                                                          >  1:173603/1‑140 (MQ=255)               aaTAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACt                                                                                                                        <  1:499401/140‑1 (MQ=255)               aaTAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACt                                                                                                                        <  1:1162893/140‑1 (MQ=255)                aTAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTc                                                                                                                           <  1:1310947/136‑1 (MQ=255)                aTAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTc                                                                                                                           >  2:1310947/1‑136 (MQ=255)                   aGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTAt                                                                                                                    <  1:1506314/140‑1 (MQ=255)                   aGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTAt                                                                                                                    <  2:1377380/140‑1 (MQ=255)                    gAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATc                                                                                                                   <  1:35686/140‑1 (MQ=255)                       aaTGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGTCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAgg                                                                                                                >  2:1428664/1‑140 (MQ=255)                       aaTGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAgg                                                                                                                <  2:1448750/140‑1 (MQ=255)                            ctctTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTaa                                                                                                           <  1:836643/140‑1 (MQ=255)                               ttGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGa                                                                                                        <  1:505291/140‑1 (MQ=255)                               ttGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGa                                                                                                        >  2:592433/1‑140 (MQ=255)                                 gTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAg                                                                                                      <  2:1136078/140‑1 (MQ=255)                                     cGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCAt                                                                                                  >  1:200561/1‑140 (MQ=255)                                     cGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCAt                                                                                                  <  2:1387263/140‑1 (MQ=255)                                       tttCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCAtt                                                                                                 >  2:1033739/1‑139 (MQ=255)                                       tttCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCAtt                                                                                                 <  1:1033739/139‑1 (MQ=255)                                       tttCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTc                                                                                                <  1:592433/140‑1 (MQ=255)                                        ttCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCa                                                                                               <  2:512292/140‑1 (MQ=255)                                           aGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGc                                                                                            <  2:73029/140‑1 (MQ=255)                                             ttttAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGt                                                                                          <  2:1445311/140‑1 (MQ=255)                                             ttttAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGt                                                                                          <  2:1456714/140‑1 (MQ=255)                                               ttAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTaa                                                                                        <  1:124449/140‑1 (MQ=255)                                                  ggCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTaa                                                                                        <  1:854651/137‑1 (MQ=255)                                                  ggCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTaa                                                                                        >  2:854651/1‑137 (MQ=255)                                                  ggCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAg                                                                                     <  1:1216597/140‑1 (MQ=255)                                                     gTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTaa                                                                                  <  1:231977/140‑1 (MQ=255)                                                       aCATTAGCGCCGTCTCGAGCCGTTTAGCTCAGAATTCTGGGGCGATGCCCACGGTAAACTACAGGGCTTTCAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAAtt                                                                                >  2:365326/1‑140 (MQ=255)                                                       aCATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAAtt                                                                                <  1:692123/140‑1 (MQ=255)                                                            aGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTAcgcg                                                                           <  1:846129/140‑1 (MQ=255)                                                            aGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTAcgcg                                                                           <  1:769095/140‑1 (MQ=255)                                                             gcgcCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCA‑GTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGga                                                                         <  1:826898/140‑1 (MQ=255)                                                                   tctcGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGAt                                                                    >  2:1185874/1‑140 (MQ=255)                                                                    ctcGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATg                                                                   <  1:1185874/140‑1 (MQ=255)                                                                    ctcGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATg                                                                   <  1:1428664/140‑1 (MQ=255)                                                                        aGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTATAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTaaa                                                               >  1:15776/1‑140 (MQ=255)                                                                           aGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTg                                                            >  1:1321970/1‑140 (MQ=255)                                                                                       aaTTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCttt                                                >  1:669719/1‑140 (MQ=255)                                                                                       aaTTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCttt                                                >  2:1283948/1‑140 (MQ=255)                                                                                       aaTTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCttt                                                >  1:149122/1‑140 (MQ=255)                                                                                       aaTTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCttt                                                >  1:1069971/1‑140 (MQ=255)                                                                                        aTTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTa                                               <  2:149122/140‑1 (MQ=255)                                                                                            tGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAat                                                        >  2:540989/1‑127 (MQ=255)                                                                                            tGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAat                                                        <  1:540989/127‑1 (MQ=255)                                                                                            tGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATggg                                           <  2:1369312/140‑1 (MQ=255)                                                                                             ggggTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGc                                          <  1:1382918/140‑1 (MQ=255)                                                                                               ggTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCAt                                        <  2:989827/140‑1 (MQ=255)                                                                                               ggTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCAt                                        <  2:1142314/140‑1 (MQ=255)                                                                                               ggTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCAt                                        <  1:263297/140‑1 (MQ=255)                                                                                                   aTGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCt                                    >  1:1194579/1‑140 (MQ=255)                                                                                                       ccACGGTAAAATACAGGGCTTATAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTa                                <  2:15776/140‑1 (MQ=255)                                                                                                                aaTACAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCg                       >  2:86227/1‑140 (MQ=255)                                                                                                                   aCAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGttt                    >  1:924326/1‑140 (MQ=255)                                                                                                                    cAGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTg                   <  2:669719/140‑1 (MQ=255)                                                                                                                     aGGGCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTgg                  >  2:558/1‑140 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCttt                                  >  2:940649/1‑122 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCttt                                  <  1:940649/122‑1 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGtt                     <  1:659832/135‑1 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGtt                     >  2:659832/1‑135 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGc                >  1:925013/1‑140 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGc                <  1:1283948/140‑1 (MQ=255)                                                                                                                       ggCTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCCTTATGGGCATTTCTTTTATGGTCATCGTTTGGGc                >  1:981131/1‑140 (MQ=255)                                                                                                                         cTTTTAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCg              >  2:841734/1‑140 (MQ=255)                                                                                                                          ttttAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCgg             <  2:173603/140‑1 (MQ=255)                                                                                                                            ttAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTa           >  2:1024073/1‑140 (MQ=255)                                                                                                                            ttAAGGTCGCTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTa           <  2:1194579/140‑1 (MQ=255)                                                                                                                                     cTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGccc  <  1:558/140‑1 (MQ=255)                                                                                                                                     cTTTGTCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGccc  >  1:396322/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGTTACTAAAAGTAAATAAGAAAATGCCTCTTGTCTCGTTTCAGTTTTAGGCGTACATTAGCGCCGTCTCGAGCAGTTTAGCTCAGAATTCTGGGGTGATGCCCACGGTAAAATACAGGGCTTTTAAGGTCGCTTTGCCCGGTGTCAACTCACTGTATCAGGTGTAATGAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCC  >  NC_000913/2496710‑2496981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑