BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,528,944	A→G	C411C (TGT→TGC)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,528,944	0	A	G	99.1%	401.8 / NA	115	C411C (TGT→TGC)	ligA	DNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (51/63); minor base T (0/1); total (51/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGA  >  NC_000913/2528807‑2529079

ttGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACcgca                                                                                                                                       >  1:930222/1‑140 (MQ=255)

ttGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACcgca                                                                                                                                       >  2:1183127/1‑140 (MQ=255)

    cGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATAcc                                                                                                                                   <  2:215898/140‑1 (MQ=255)

        cATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGAc                                                                                                                               >  1:1058154/1‑140 (MQ=255)

        cATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGAc                                                                                                                               <  2:849227/140‑1 (MQ=255)

        cATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGAc                                                                                                                               >  2:557106/1‑140 (MQ=255)

         aTTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACa                                                                                                                              >  1:1085644/1‑140 (MQ=255)

             cGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGc                                                                                                                          <  1:177571/140‑1 (MQ=255)

             cGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGc                                                                                                                          <  1:129183/140‑1 (MQ=255)

                cAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTc                                                                                                                       <  2:1073712/140‑1 (MQ=255)

                cAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTc                                                                                                                       <  2:242450/140‑1 (MQ=255)

                 aaCATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCg                                                                                                                      <  2:287771/140‑1 (MQ=255)

                  aCATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCgg                                                                                                                     >  2:63314/1‑140 (MQ=255)

                   catccatcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCggg                                                                                                                    >  1:195003/1‑140 (MQ=255)

                    atccatcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGa                                                                                                                   >  1:752375/1‑140 (MQ=255)

                     tccatcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAGCCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGaa                                                                                                                  >  2:1343183/1‑140 (MQ=255)

                     tccatcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGt                                                                                                                        <  2:915303/134‑1 (MQ=255)

                     tccatcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGaa                                                                                                                  >  2:901447/1‑140 (MQ=255)

                       catcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATa                                                                                                                >  1:785071/1‑140 (MQ=255)

                        atcGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATAc                                                                                                               <  1:557106/140‑1 (MQ=255)

                            cACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAAcc                                                                                                           >  1:938241/1‑140 (MQ=255)

                            cACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAAcc                                                                                                           >  2:1260050/1‑140 (MQ=255)

                            cACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTAACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAAcc                                                                                                           <  2:838106/140‑1 (MQ=255)

                             aCGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCgg                                                                                                                     <  1:915868/129‑1 (MQ=255)

                             aCGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCt                                                                                                          <  2:635238/140‑1 (MQ=255)

                             aCGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCt                                                                                                          <  2:960755/140‑1 (MQ=255)

                             aCGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCt                                                                                                          >  1:331764/1‑140 (MQ=255)

                               ggcggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCa                                                                                                        <  2:559332/140‑1 (MQ=255)

                                 cggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACg                                                                                                      >  1:1155557/1‑140 (MQ=255)

                                 cggGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACg                                                                                                      >  2:58158/1‑140 (MQ=255)

                                  gggAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACgg                                                                                                     <  1:864608/140‑1 (MQ=255)

                                   ggAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACggg                                                                                                    <  2:1355087/140‑1 (MQ=255)

                                          aaGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTc                                                                                             <  2:925597/140‑1 (MQ=255)

                                           aGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTcc                                                                                            >  2:851585/1‑140 (MQ=255)

                                            gtgtTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCg                                                                                           <  1:1260050/140‑1 (MQ=255)

                                              gtTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCggg                                                                                         >  2:100803/1‑140 (MQ=255)

                                                ttCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCg                                                                                       >  2:774439/1‑140 (MQ=255)

                                                      gACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGa                                                                                 <  1:901447/140‑1 (MQ=255)

                                                       aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGaa                                                                                >  1:1399559/1‑140 (MQ=255)

                                                          ctTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCTCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGc                                                                             <  1:1205182/140‑1 (MQ=255)

                                                           tttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            <  2:1382014/140‑1 (MQ=255)

                                                           tttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            <  2:321379/140‑1 (MQ=255)

                                                           tttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            <  2:195003/140‑1 (MQ=255)

                                                            ttACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCac                                                                           <  1:1057889/140‑1 (MQ=255)

                                                              aCGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCacga                                                                         <  2:752375/140‑1 (MQ=255)

                                                              aCGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCacga                                                                         <  2:523717/140‑1 (MQ=255)

                                                              aCGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCacga                                                                         <  2:1058154/140‑1 (MQ=255)

                                                                  tGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            <  2:394427/133‑1 (MQ=255)

                                                                  tGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            >  1:394427/1‑133 (MQ=255)

                                                                   gcgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTa                                                                    >  1:846773/1‑140 (MQ=255)

                                                                   gcgcACCCCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTa                                                                    >  2:1183623/1‑140 (MQ=255)

                                                                    cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCacg                                                                          >  1:585049/1‑133 (MQ=255)

                                                                    cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTaa                                                                   >  2:491945/1‑140 (MQ=255)

                                                                    cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTaa                                                                   >  1:1295958/1‑140 (MQ=255)

                                                                    cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTaa                                                                   >  2:1123493/1‑140 (MQ=255)

                                                                    cgcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTaa                                                                   >  1:1239604/1‑140 (MQ=255)

                                                                     gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCa                                                                            <  1:490646/130‑1 (MQ=255)

                                                                     gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAac                                                                  <  1:86000/140‑1 (MQ=255)

                                                                     gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAac                                                                  >  1:1018207/1‑140 (MQ=255)

                                                                      cACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAacc                                                                 <  1:865558/140‑1 (MQ=255)

                                                                         cGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAaccacc                                                              >  2:14335/1‑140 (MQ=255)

                                                                           cAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTg                                                            <  1:1260019/140‑1 (MQ=255)

                                                                             aaTCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCg                                                          <  1:491945/140‑1 (MQ=255)

                                                                               tCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg                                                        <  1:1123493/140‑1 (MQ=255)

                                                                               tCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTACggg                                                        <  2:1399559/140‑1 (MQ=255)

                                                                               tCAGGACACCGGTACAGCGGGCAACCTCTTCACCTTCCACTCGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTTCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCggg                                                        <  2:1356586/140‑1 (MQ=255)

                                                                                   gCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTg                                                            <  2:420255/132‑1 (MQ=255)

                                                                                   gCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTg                                                            >  1:420255/1‑132 (MQ=255)

                                                                                    ccACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCAc                                                   <  1:1478750/140‑1 (MQ=255)

                                                                                             cAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAgc                                          >  2:898780/1‑140 (MQ=255)

                                                                                              aGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAgcg                                         <  1:1183623/140‑1 (MQ=255)

                                                                                               gCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAgcgc                                        <  2:930222/140‑1 (MQ=255)

                                                                                                 gggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                      >  1:1178118/1‑140 (MQ=255)

                                                                                                 gggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                      <  1:774439/140‑1 (MQ=255)

                                                                                                 gggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                      <  2:1155557/140‑1 (MQ=255)

                                                                                                  ggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcggc                                     >  1:174188/1‑140 (MQ=255)

                                                                                                  ggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcggc                                     >  1:384045/1‑140 (MQ=255)

                                                                                                  ggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcggc                                     >  2:1396932/1‑140 (MQ=255)

                                                                                                    cAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGa                                   <  1:58158/140‑1 (MQ=255)

                                                                                                          cTTCACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc                             <  2:21732/140‑1 (MQ=255)

                                                                                                             cACCTTCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACt                          >  1:929029/1‑140 (MQ=255)

                                                                                                                 ttCCACACGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTAt                      <  2:174188/140‑1 (MQ=255)

                                                                                                                     acacGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGcc                  >  2:1533926/1‑140 (MQ=255)

                                                                                                                     acacGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGcc                  >  2:1045204/1‑140 (MQ=255)

                                                                                                                     acacGCTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGcc                  <  1:851585/140‑1 (MQ=255)

                                                                                                                          cTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc                             <  1:953724/124‑1 (MQ=255)

                                                                                                                          cTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcacc                             >  2:953724/1‑124 (MQ=255)

                                                                                                                          cTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa                 <  2:500294/136‑1 (MQ=255)

                                                                                                                          cTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCa                 >  1:500294/1‑136 (MQ=255)

                                                                                                                          cTCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGc             >  1:1298140/1‑140 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  2:938241/140‑1 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  1:1045204/140‑1 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  1:14335/140‑1 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  1:350271/140‑1 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  2:1295958/140‑1 (MQ=255)

                                                                                                                           tCAACGTCAGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCg            <  1:898780/140‑1 (MQ=255)

                                                                                                                                   aGAACCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCaa    >  2:292954/1‑140 (MQ=255)

                                                                                                                                     aaCCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCa     <  2:661864/137‑1 (MQ=255)

                                                                                                                                     aaCCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCa     >  1:661864/1‑137 (MQ=255)

                                                                                                                                     aaCCGCATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGa  >  1:1050180/1‑140 (MQ=255)

TTGTCGCCCATTCCGTCAACATCCATCGCACGGCGGGAAACAAAGTGTTTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGA  >  NC_000913/2528807‑2529079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`