Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,001,665	T→C	Y441H (TAC→CAC)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,001,665	0	T	C	100.0%	144.5 / NA	42	Y441H (TAC→CAC)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

GCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCTACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGT  >  NC_000913/3001528‑3001786                                                                                                                                          |                                                                                                                          gCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCac                                                                                                                         <  1:490065/140‑1 (MQ=255)  cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  2:196710/140‑1 (MQ=255)  cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  1:988543/140‑1 (MQ=255)  cAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCcaca                                                                                                                        <  1:35796/140‑1 (MQ=255)       gTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACctct                                                                                                                   <  2:1361455/140‑1 (MQ=255)               aGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTg                                                                                                           >  1:264954/1‑140 (MQ=255)                 cgcAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGc                                                                                                         >  2:990367/1‑140 (MQ=255)                 cgcAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGc                                                                                                         >  2:533589/1‑140 (MQ=255)                   cAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCt                                                                                                       >  2:706759/1‑140 (MQ=255)                     gggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGt                                                                                                     <  2:1041612/140‑1 (MQ=255)                      ggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTaa                                                                                                                 <  1:1248305/127‑1 (MQ=255)                      ggTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTaa                                                                                                                 >  2:1248305/1‑127 (MQ=255)                        ttGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCgg                                                                                                  <  1:1014889/140‑1 (MQ=255)                        ttGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCgg                                                                                                  <  1:990367/140‑1 (MQ=255)                         tGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGc                                                                                                 <  2:992156/140‑1 (MQ=255)                           ccGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGt                                                                                               >  1:390794/1‑140 (MQ=255)                             ggAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAg                                                                                             >  2:468807/1‑140 (MQ=255)                                gtgtCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGaaa                                                                                          >  1:1490467/1‑140 (MQ=255)                                 tgtCTTGAAAAAGGCCGGAAAATCTTTTAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAAt                                                                                         >  2:1421512/1‑140 (MQ=255)                                 tgtCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAAt                                                                                         >  2:1076617/1‑140 (MQ=255)                                           aaGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcg                                                                               <  2:1490467/140‑1 (MQ=255)                                            aGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcgc                                                                              >  2:685177/1‑140 (MQ=255)                                            aGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGgcgcgc                                                                              <  1:1094679/140‑1 (MQ=255)                                               ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  1:105124/140‑1 (MQ=255)                                               ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  2:84798/140‑1 (MQ=255)                                               ccGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGcc                                                                           <  1:468807/140‑1 (MQ=255)                                                     aaTCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCtga                                                                     <  1:533589/140‑1 (MQ=255)                                                          ttGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAAt                                                                <  2:390794/140‑1 (MQ=255)                                                          ttGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAAt                                                                <  1:706759/140‑1 (MQ=255)                                                                 ggAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATg                                                         >  2:96628/1‑140 (MQ=255)                                                                  gAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATgg                                                        <  1:1421512/140‑1 (MQ=255)                                                                     aaaCGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAAc                                                     >  1:153687/1‑140 (MQ=255)                                                                       aCGCCGTGCGGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGAGCCTTCTGATGAATCAGGATGGAACCa                                                   <  2:508591/140‑1 (MQ=255)                                                                        cGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCAt                                                  <  2:153687/140‑1 (MQ=255)                                                                            gTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGAt                                                          >  2:846596/1‑128 (MQ=255)                                                                            gTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGAt                                                          <  1:846596/128‑1 (MQ=255)                                                                                            ccAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGa                              >  1:1110477/1‑140 (MQ=255)                                                                                            ccAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAAAACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGa                              >  1:1415492/1‑140 (MQ=255)                                                                                                    gCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATc                      >  1:1090219/1‑140 (MQ=255)                                                                                                     cAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCg                     >  2:1384305/1‑140 (MQ=255)                                                                                                        tttGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTc                  <  1:1384305/140‑1 (MQ=255)                                                                                                         ttGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCa                 <  1:1076617/140‑1 (MQ=255)                                                                                                            cgccgcGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAggg              <  2:1090219/140‑1 (MQ=255)                                                                                                              ccgcGGCGTTGGCGTCGCCTGTTTTAGCCACACCTCTAAAACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTg            <  2:1415492/140‑1 (MQ=255)                                                                                                                        ggCGTCGCCTGTTTTAGCCACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGt  <  2:402069/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                          GCAAACGTATTTACAGCGCAGGGTTGCCGGAGTGTCTTGAAAAAGGCCGGAAAATCTTTGAATGGGAAAAACGCCGTGCAGAATGCCAGAACCAGCAAGGCAATTTGCGCCGCGGCGTTGGCGTCGCCTGTTTTAGCTACACCTCTAACACCTGGCCTGTCGGCGTAGAAATAGCAGGCGCGCGCCTTCTGATGAATCAGGATGGAACCATCAACGTGCAAAGCGGCGCGACGGAAATCGGTCAGGGTGCCGACACCGT  >  NC_000913/3001528‑3001786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑