Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,056,598	T→C	F120L (TTT→CTT)	fau →	putative 5‑formyltetrahydrofolate cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,056,598	0	T	C	100.0%	276.7 / NA	80	F120L (TTT→CTT)	fau	putative 5‑formyltetrahydrofolate cyclo‑ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/37);  total (43/37)

GTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGAAGA  >  NC_000913/3056461‑3056734                                                                                                                                          |                                                                                                                                         gTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCctt                                                                                                                                        >  1:631127/1‑140 (MQ=255) gTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCctt                                                                                                                                        >  2:658420/1‑140 (MQ=255)               cTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCa                                                                                                                          <  2:776557/140‑1 (MQ=255)               cTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCa                                                                                                                          <  2:158068/140‑1 (MQ=255)               cTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCa                                                                                                                          <  2:492657/140‑1 (MQ=255)               cTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCa                                                                                                                          <  1:1359307/140‑1 (MQ=255)                      aCCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTg                                                                                                                   >  1:116212/1‑140 (MQ=255)                        cATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTggg                                                                                                                 >  2:158676/1‑140 (MQ=255)                        cATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTggg                                                                                                                 >  2:483839/1‑140 (MQ=255)                         aTCCGCAGAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATAAGTACGGTCAGTGCCTGGGa                                                                                                                >  1:1163014/1‑140 (MQ=255)                         aTCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCt                                                                                                                    >  2:1195760/1‑136 (MQ=255)                         aTCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCt                                                                                                                    <  1:1195760/136‑1 (MQ=255)                         aTCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGa                                                                                                                >  2:1335465/1‑140 (MQ=255)                          tCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCt                                                                                                                    <  2:1878/135‑1 (MQ=255)                          tCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCt                                                                                                                    >  1:1878/1‑135 (MQ=255)                                aaGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCg                                                                                                         <  1:16551/140‑1 (MQ=255)                                 aGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCgg                                                                                                        >  1:869590/1‑140 (MQ=255)                                 aGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCgg                                                                                                        <  2:631127/140‑1 (MQ=255)                                         ggTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGttttt                                                                                                <  2:54712/140‑1 (MQ=255)                                                aaCAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCg                                                                                         >  2:1129458/1‑140 (MQ=255)                                                aaCAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCg                                                                                         <  1:1267791/140‑1 (MQ=255)                                                    ggTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                                     >  2:629494/1‑140 (MQ=255)                                                     gTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGATGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCt                                                                                    >  1:1367761/1‑140 (MQ=255)                                                             tCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAAc                                                                            >  1:261641/1‑140 (MQ=255)                                                               cATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGCGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGGTCGGTCCTTAACAAACTg                                                                          >  2:1523199/1‑140 (MQ=255)                                                               cATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTg                                                                          >  1:361434/1‑140 (MQ=255)                                                               cATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTg                                                                          >  1:953780/1‑140 (MQ=255)                                                               cATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTg                                                                          >  1:920616/1‑140 (MQ=255)                                                                aTGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTgg                                                                         <  2:116212/140‑1 (MQ=255)                                                                aTGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTgg                                                                         <  1:629494/140‑1 (MQ=255)                                                                 tGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgc                                                                        >  1:661620/1‑140 (MQ=255)                                                                   aGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTgg                                                                         <  1:276680/137‑1 (MQ=255)                                                                   aGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTgg                                                                         >  2:276680/1‑137 (MQ=255)                                                                   aGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcag                                                                      >  2:1315994/1‑140 (MQ=255)                                                                    gCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagc                                                                     <  1:1335465/140‑1 (MQ=255)                                                                     ccAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagca                                                                    >  1:235709/1‑140 (MQ=255)                                                                               aTGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACg                                                          <  2:235709/140‑1 (MQ=255)                                                                               aTGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATAAGTACGGTCAGTGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACg                                                          <  2:1163014/140‑1 (MQ=255)                                                                                tgtgCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                                         <  1:483839/140‑1 (MQ=255)                                                                                tgtgCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                                         <  2:261641/140‑1 (MQ=255)                                                                                tgtgCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                                         <  1:158676/140‑1 (MQ=255)                                                                                   gCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTCTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAAc                                                      >  1:1338750/1‑140 (MQ=255)                                                                                   gCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGAACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAAc                                                      >  2:1046672/1‑140 (MQ=255)                                                                                       gACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGt                                                  <  2:361434/140‑1 (MQ=255)                                                                                        aCGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTg                                                 <  1:1498364/140‑1 (MQ=255)                                                                                            gCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                                         <  1:1415568/128‑1 (MQ=255)                                                                                            gCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                                         >  2:1415568/1‑128 (MQ=255)                                                                                            gCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGtt                                             <  2:661620/140‑1 (MQ=255)                                                                                             cTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGTGCATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTa                                            >  2:1209108/1‑140 (MQ=255)                                                                                             cTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTa                                            >  1:978266/1‑140 (MQ=255)                                                                                               aCCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATTGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATg                                          >  1:1092377/1‑140 (MQ=255)                                                                                                   cTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCa                                      <  1:1129458/140‑1 (MQ=255)                                                                                                     ttCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTg                                                 <  2:425000/127‑1 (MQ=255)                                                                                                     ttCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTg                                                 >  1:425000/1‑127 (MQ=255)                                                                                                     ttCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATg                                    >  2:244878/1‑140 (MQ=255)                                                                                                     ttCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATg                                    >  2:496175/1‑140 (MQ=255)                                                                                                      tCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTggg                                               >  1:865127/1‑128 (MQ=255)                                                                                                      tCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTggg                                               <  2:865127/128‑1 (MQ=255)                                                                                                      tCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCa                                      <  2:1362533/137‑1 (MQ=255)                                                                                                      tCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCa                                      >  1:1362533/1‑137 (MQ=255)                                                                                                           aTTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATg                                          >  2:982275/1‑128 (MQ=255)                                                                                                           aTTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATg                                          <  1:982275/128‑1 (MQ=255)                                                                                                           aTTAGACGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTc                              <  2:953780/140‑1 (MQ=255)                                                                                                                aCGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTtg                         >  2:965990/1‑140 (MQ=255)                                                                                                                 cGTGCTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTtgg                        <  2:1367761/140‑1 (MQ=255)                                                                                                                     cTGATCACACCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGa                    >  1:963214/1‑140 (MQ=255)                                                                                                                           acacCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACt              <  1:1044147/140‑1 (MQ=255)                                                                                                                             acCGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTcc            <  1:658420/140‑1 (MQ=255)                                                                                                                               cGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTcccc          >  1:559290/1‑140 (MQ=255)                                                                                                                               cGCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTcccc          >  2:1235483/1‑140 (MQ=255)                                                                                                                                gCTGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCg         <  2:869590/140‑1 (MQ=255)                                                                                                                                  tGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTcccc          <  1:460823/137‑1 (MQ=255)                                                                                                                                  tGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTcccc          >  2:460823/1‑137 (MQ=255)                                                                                                                                  tGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGtt       >  1:479633/1‑140 (MQ=255)                                                                                                                                  tGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGtt       >  1:1234383/1‑140 (MQ=255)                                                                                                                                  tGGTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGtt       >  1:752627/1‑140 (MQ=255)                                                                                                                                   ggTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTg      <  2:695577/140‑1 (MQ=255)                                                                                                                                   ggTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTg      <  2:963214/140‑1 (MQ=255)                                                                                                                                    gTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGa     >  2:810908/1‑140 (MQ=255)                                                                                                                                    gTCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGa     >  2:1158925/1‑140 (MQ=255)                                                                                                                                     tCGCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAAATGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGaa    >  2:1392308/1‑140 (MQ=255)                                                                                                                                       gCCCTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGAaga  <  1:496175/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAACTCCCCGTTGAAGA  >  NC_000913/3056461‑3056734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑