Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,116	C→T	R146C (CGT→TGT)	rdgB →	dITP/XTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,116	0	C	T	100.0%	271.1 / NA	76	R146C (CGT→TGT)	rdgB	dITP/XTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/26);  total (50/26)

AACTGCTGGAAACAATGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTCGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAACTGCT  >  NC_000913/3096982‑3097249                                                                                                                                         |                                                                                                                                      aaCTGCTGGAAACAATGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGaa                                                                                                                                  <  2:921352/140‑1 (MQ=255) aaCTGCTGGAAACAATGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGaa                                                                                                                                  <  2:1499748/140‑1 (MQ=255)         gAAACAGTGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCggg                                                                                                                          <  1:778297/140‑1 (MQ=255)                tGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtggt                                                                                                                   <  2:923174/140‑1 (MQ=255)                tGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtggt                                                                                                                   <  1:1376945/140‑1 (MQ=255)                tGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtggt                                                                                                                   >  1:862947/1‑140 (MQ=255)                tGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtggt                                                                                                                   >  1:997626/1‑140 (MQ=255)                tGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCATCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtggt                                                                                                                   <  2:1372218/140‑1 (MQ=255)                    aGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCt                                                                                                               <  2:1226055/140‑1 (MQ=255)                     gACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCtt                                                                                                              <  2:489477/140‑1 (MQ=255)                        gTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCggg                                                                                                                          <  1:780556/125‑1 (MQ=255)                        gTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCggg                                                                                                                          >  2:780556/1‑125 (MQ=255)                        gTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCt                                                                                                               >  1:585340/1‑136 (MQ=255)                        gTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCt                                                                                                               <  2:585340/136‑1 (MQ=255)                          aCCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGtt                                                                                                         >  2:1447420/1‑140 (MQ=255)                          aCCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGtt                                                                                                         >  2:313853/1‑140 (MQ=255)                          aCCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGtt                                                                                                         >  1:958060/1‑140 (MQ=255)                           ccGGACGACAAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTa                                                                                                        <  2:1468822/140‑1 (MQ=255)                            cGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTAt                                                                                                       >  2:1318557/1‑140 (MQ=255)                              gacgacCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCt                                                                                                               <  2:1198464/130‑1 (MQ=255)                              gacgacCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCt                                                                                                               >  1:1198464/1‑130 (MQ=255)                                  acCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCGGAGAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtg                                                                                                                     <  1:1414523/122‑1 (MQ=255)                                  acCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCGGAGAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACtg                                                                                                                     >  2:1414523/1‑122 (MQ=255)                                    cAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAt                                                                                               <  2:975924/140‑1 (MQ=255)                                    cAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAt                                                                                               <  2:880971/140‑1 (MQ=255)                                    cAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAt                                                                                               <  1:313853/140‑1 (MQ=255)                                      aCGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTa                                                                                                        >  2:1322479/1‑129 (MQ=255)                                      aCGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTa                                                                                                        <  1:1322479/129‑1 (MQ=255)                                      aCGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAtct                                                                                             >  1:1286148/1‑140 (MQ=255)                                            ggCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCg                                                                                       <  1:460619/140‑1 (MQ=255)                                             gcgcGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGt                                                                                      >  1:693595/1‑140 (MQ=255)                                                  tttCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAtc                                                                                              <  1:1394128/127‑1 (MQ=255)                                                  tttCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCAGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAAtc                                                                                              >  2:1394128/1‑127 (MQ=255)                                                    tCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTcc                                                                               <  2:862947/140‑1 (MQ=255)                                                           cGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGa                                                                        >  2:1296485/1‑140 (MQ=255)                                                             tGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGaaa                                                                      >  1:592060/1‑140 (MQ=255)                                                             tGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGaaa                                                                      >  1:224752/1‑140 (MQ=255)                                                               cTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTCCTTCGTACCTTCCGAAGGGAAAAc                                                                    >  2:1105152/1‑140 (MQ=255)                                                                         tGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGaa                                                          <  2:592060/140‑1 (MQ=255)                                                                          gCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAATCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAAc                                                         >  1:1022451/1‑140 (MQ=255)                                                                            gTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTg                                                       >  2:442321/1‑140 (MQ=255)                                                                            gTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTg                                                       >  1:222845/1‑140 (MQ=255)                                                                            gTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTg                                                       >  2:301213/1‑140 (MQ=255)                                                                            gTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTg                                                       >  1:637558/1‑140 (MQ=255)                                                                                        aGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCgaag                                             >  1:744163/1‑140 (MQ=255)                                                                                                 tCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAgcg                                    >  2:692787/1‑140 (MQ=255)                                                                                                 tCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAgcg                                    >  2:1216909/1‑140 (MQ=255)                                                                                                  ccGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAgcgc                                   <  1:692787/140‑1 (MQ=255)                                                                                                   cGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGcc                                  <  2:637558/140‑1 (MQ=255)                                                                                                   cGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGcc                                  >  2:56411/1‑140 (MQ=255)                                                                                                    gCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCa                                 >  1:1296911/1‑140 (MQ=255)                                                                                                     cTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCAt                                >  2:232033/1‑140 (MQ=255)                                                                                                     cTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCAt                                >  2:249034/1‑140 (MQ=255)                                                                                                     cTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCAt                                >  1:339696/1‑140 (MQ=255)                                                                                                      tGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCAtt                               <  2:1022451/140‑1 (MQ=255)                                                                                                         tATGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTcc                            >  1:599417/1‑140 (MQ=255)                                                                                                          aTGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTccc                           >  1:254574/1‑140 (MQ=255)                                                                                                           tGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCa                          >  1:1435803/1‑140 (MQ=255)                                                                                                           tGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCa                          >  2:572368/1‑140 (MQ=255)                                                                                                           tGCCACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCa                          >  2:447596/1‑140 (MQ=255)                                                                                                             ccACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCAcc                        >  1:1149055/1‑140 (MQ=255)                                                                                                             ccACGGTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTATGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGG‑AAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCg                       >  1:1498772/1‑140 (MQ=255)                                                                                                                  gcaGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGt                   >  2:358949/3‑140 (MQ=255)                                                                                                                  gTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGCAGAAAAGATCGCCATTTCCCACCGTGGt                   >  2:1481344/1‑140 (MQ=255)                                                                                                                  gTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGt                   >  1:596312/1‑140 (MQ=255)                                                                                                                  gTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGt                   >  1:842936/1‑140 (MQ=255)                                                                                                                  gTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGt                   >  2:845554/1‑140 (MQ=255)                                                                                                                  gTAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGt                   >  2:936261/1‑140 (MQ=255)                                                                                                                   tAGCTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTc                  >  2:90718/1‑140 (MQ=255)                                                                                                                      cTGGCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAgg               >  1:282824/1‑140 (MQ=255)                                                                                                                      cTGGCCGGGCGCGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAgg               >  2:1025167/1‑140 (MQ=255)                                                                                                                         gCCGGGCGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGt            <  2:222845/140‑1 (MQ=255)                                                                                                                               cGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAAc      <  2:254574/140‑1 (MQ=255)                                                                                                                               cGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAAc      >  2:1511271/1‑140 (MQ=255)                                                                                                                               cGTGATTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAAc      >  1:1236631/1‑140 (MQ=255)                                                                                                                                   aTTACTTGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAActgct  >  1:480536/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AACTGCTGGAAACAATGAAAGACGTACCGGACGACCAACGTCAGGCGCGTTTCCACTGCGTGCTGGTATATCTGCGTCACGCG‑‑GAAGATCCCACTCCGCTGGTATGCCACGGTAGCTGGCCGGGCGTGATTACTCGTGAACCAGCGGGCACTGGTGGCTTTGGTTATGATCCAATCTTCTTCGTACCTTCCGAAGGGAAAACCGCCGCCGAACTGACCCGCGAAGAAAAGAGCGCCATTTCCCACCGTGGTCAGGCGTTGAAACTGCT  >  NC_000913/3096982‑3097249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑