BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,476	T→C	W85R (TGG→CGG)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,476	0	T	C	99.3%	464.3 / ‑5.4	135	W85R (TGG→CGG)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (78/56); total (78/57)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

GTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGA  >  NC_000913/3245340‑3245611

gTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCggg                                                                                                                                      <  1:1508061/140‑1 (MQ=255)

  tAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGcc                                                                                                                                    >  1:748504/1‑140 (MQ=255)

  tAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGcc                                                                                                                                    <  2:735631/140‑1 (MQ=255)

    aaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCgt                                                                                                                                  <  1:541658/140‑1 (MQ=255)

    aaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCgt                                                                                                                                  >  1:1237001/1‑140 (MQ=255)

     aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCgtg                                                                                                                                 <  1:9326/140‑1 (MQ=255)

     aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCgtg                                                                                                                                 >  1:1395898/1‑140 (MQ=255)

     aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCgtg                                                                                                                                 <  2:686048/140‑1 (MQ=255)

        tctcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTc                                                                                                                              <  1:65706/140‑1 (MQ=255)

          tcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAATTCGGTAATGCATTGTTTGCTGTGCTGCGGGCCGGGTTCtg                                                                                                                            >  1:1229104/1‑140 (MQ=255)

          tcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCtg                                                                                                                            <  2:880254/140‑1 (MQ=255)

          tcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCtg                                                                                                                            <  2:459164/140‑1 (MQ=255)

          tcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTACTGTGCTGCGGGCCGTGTTCtg                                                                                                                            >  1:1007284/1‑140 (MQ=255)

                 aaCAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCa                                                                                                                     <  2:748504/140‑1 (MQ=255)

                   cAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAAc                                                                                                                   >  1:895350/1‑140 (MQ=255)

                   cAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAAc                                                                                                                   >  2:133864/1‑140 (MQ=255)

                     gTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACcg                                                                                                                 <  2:805152/140‑1 (MQ=255)

                      tATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACcgc                                                                                                                >  1:639365/1‑140 (MQ=255)

                        ttCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCgcgc                                                                                                              >  2:1084207/1‑140 (MQ=255)

                           cTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTgg                                                                                                           >  2:1497927/1‑140 (MQ=255)

                             atatCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCa                                                                                                         <  2:595745/140‑1 (MQ=255)

                             atatCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGCAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCa                                                                                                         >  2:20146/1‑140 (MQ=255)

                              tatCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAg                                                                                                        >  2:755730/1‑140 (MQ=255)

                                tcatcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGt                                                                                                      >  1:1361292/1‑140 (MQ=255)

                                tcatcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGt                                                                                                      >  1:917896/1‑140 (MQ=255)

                                  atcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAg                                                                                                    >  2:450904/1‑140 (MQ=255)

                                  atcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAg                                                                                                    >  1:1359546/1‑140 (MQ=255)

                                  atcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTAAGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAg                                                                                                    >  2:662688/1‑140 (MQ=255)

                                   tcGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGc                                                                                                   >  1:896867/1‑140 (MQ=255)

                                    cGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCa                                                                                                         >  1:1503856/1‑133 (MQ=255)

                                    cGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCt                                                                                                  >  1:1335701/1‑140 (MQ=255)

                                        gCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGGTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAg                                                                                                    >  2:564472/1‑134 (MQ=255)

                                        gCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTgggg                                                                                              >  2:1454103/1‑140 (MQ=255)

                                         ccTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGt                                                                                             >  2:812036/1‑140 (MQ=255)

                                             ttCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCTGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTggg                                                                                               <  1:300072/134‑1 (MQ=255)

                                             ttCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCTGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTggg                                                                                               >  2:300072/1‑134 (MQ=255)

                                                  ctgctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCt                                                                                    >  2:669663/1‑140 (MQ=255)

                                                  ctgctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCt                                                                                    >  1:1276868/1‑140 (MQ=255)

                                                   tgctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTg                                                                                   >  1:974668/1‑140 (MQ=255)

                                                   tgctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTg                                                                                   >  1:692052/1‑140 (MQ=255)

                                                   tgctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGAGGTGGCCTGGCTg                                                                                   >  1:1424264/1‑140 (MQ=255)

                                                    gctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  <  2:365095/140‑1 (MQ=255)

                                                    gctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  >  1:1009399/1‑140 (MQ=255)

                                                    gctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  <  1:1497927/140‑1 (MQ=255)

                                                    gctTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  <  1:1454103/140‑1 (MQ=255)

                                                        ataCGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCt                                                                              <  2:1237001/140‑1 (MQ=255)

                                                        ataCGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCt                                                                              <  2:1201571/140‑1 (MQ=255)

                                                           cGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCgt                                                                           <  1:444842/140‑1 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCt                                                                              <  2:1001671/133‑1 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCt                                                                              >  1:1001671/1‑133 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCgtggtg                                                                       <  2:896867/140‑1 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCgtggtg                                                                       <  2:589115/140‑1 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCgtggtg                                                                       >  1:589115/1‑140 (MQ=255)

                                                               cATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCgtggtg                                                                       >  2:1313564/1‑140 (MQ=255)

                                                                aTGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGc                                                                      >  1:237806/1‑140 (MQ=255)

                                                                aTGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGc                                                                      >  2:1279518/1‑140 (MQ=255)

                                                                 tGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  <  2:1165259/127‑1 (MQ=255)

                                                                 tGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGt                                                                                  >  1:1165259/1‑127 (MQ=255)

                                                                      ccGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGcggtcgg                                                                >  1:1387153/1‑140 (MQ=255)

                                                                       cGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGcggtcggt                                                               <  2:795639/140‑1 (MQ=255)

                                                                         gTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGc                                                             <  2:639365/140‑1 (MQ=255)

                                                                                gTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGaa                                                      >  1:458141/1‑140 (MQ=255)

                                                                                gTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGaa                                                      >  2:884913/1‑140 (MQ=255)

                                                                                  tATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAagc                                                    <  1:1423891/140‑1 (MQ=255)

                                                                                    tGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAag                                                     >  2:1402671/1‑137 (MQ=255)

                                                                                    tGTACTGGATGGGCTGGGGACGAAATTAGGTTATCCAATGTTTGCTGTGTTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGGAGGAAGCTTGGGCGGCCTGGCTGTTGCTCGTGGTTAGGTCGGGGCCGCGGAagcag                                                  >  2:836134/1‑140 (MQ=255)

                                                                                         tGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCgatg                                             >  1:1203949/1‑140 (MQ=255)

                                                                                           gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCgatgat                                           >  1:1096613/1‑140 (MQ=255)

                                                                                           gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCgatgat                                           >  1:1329680/1‑140 (MQ=255)

                                                                                           gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCgatgat                                           >  2:1253666/1‑140 (MQ=255)

                                                                                           gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCgatgat                                           >  1:69469/1‑140 (MQ=255)

                                                                                           gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCg                                                <  2:1384059/135‑1 (MQ=255)

                                                                                              gtgCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAagca                                                   <  2:8180/129‑1 (MQ=255)

                                                                                              gtgCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAagca                                                   >  1:8180/1‑129 (MQ=255)

                                                                                              gtgCTGGGTACGAAAATCGGTTATGCAATG‑TTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcg                                       >  1:551425/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  2:718698/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  2:989777/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  2:987348/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  2:1302841/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  1:623872/1‑140 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      <  2:1387153/140‑1 (MQ=255)

                                                                                                gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcgg                                      >  2:1440635/1‑140 (MQ=255)

                                                                                                 cTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCcggc                                     <  1:901567/140‑1 (MQ=255)

                                                                                                     gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCg                                                >  1:564772/1‑125 (MQ=255)

                                                                                                     gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGt                                 >  1:1479424/1‑140 (MQ=255)

                                                                                                     gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGt                                 >  1:1271198/1‑140 (MQ=255)

                                                                                                        cGAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTg                              <  2:1334443/140‑1 (MQ=255)

                                                                                                         gAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGa                             >  2:1480379/1‑140 (MQ=255)

                                                                                                         gAAAATCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGa                             >  2:1327453/1‑140 (MQ=255)

                                                                                                            aaTCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAg                          <  2:1271198/140‑1 (MQ=255)

                                                                                                             aTCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGc                         <  1:812036/140‑1 (MQ=255)

                                                                                                              tCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGcc                        <  1:734307/140‑1 (MQ=255)

                                                                                                              tCGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGcc                        >  1:1474706/1‑140 (MQ=255)

                                                                                                               cGGTTATGCAATGTTTGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCa                       <  2:1096613/140‑1 (MQ=255)

                                                                                                                            tttGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGCTCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt          <  2:1359546/140‑1 (MQ=255)

                                                                                                                            tttGCTGTGCTGCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt          <  2:906561/140‑1 (MQ=255)

                                                                                                                              tgctgtgctgCGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTcc        <  1:989777/140‑1 (MQ=255)

                                                                                                                                ctgtgctgcgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCg      <  1:1327453/140‑1 (MQ=255)

                                                                                                                                  gtgctgcgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGc    >  2:52513/1‑140 (MQ=255)

                                                                                                                                    gctgcgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGa  >  1:762594/1‑140 (MQ=255)

GTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGA  >  NC_000913/3245340‑3245611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`