Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,314,509	T→C	K502K (AAA→AAG)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,314,509	0	T	C	100.0%	178.1 / NA	52	K502K (AAA→AAG)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/24);  total (28/24)

AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCA  >  NC_000913/3314374‑3314644                                                                                                                                        |                                                                                                                                        aGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTAt                                                                                                                                     <  2:1473280/140‑1 (MQ=255) aGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTAt                                                                                                                                     <  1:105701/140‑1 (MQ=255)   cGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCg                                                                                                                                   >  2:281628/1‑140 (MQ=255)   cGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCg                                                                                                                                   >  2:616496/1‑140 (MQ=255)     tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGAt                                                                                                                                 >  2:1452570/1‑140 (MQ=255)     tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGAt                                                                                                                                 >  2:1452866/1‑140 (MQ=255)      ccAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCg                                                                                                                                   <  2:1225282/137‑1 (MQ=255)      ccAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCg                                                                                                                                   >  1:1225282/1‑137 (MQ=255)        agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTAt                                                                                                                                     >  1:142431/1‑133 (MQ=255)        agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTAt                                                                                                                                     <  2:142431/133‑1 (MQ=255)         gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTg                                                                                                                             >  2:526972/1‑140 (MQ=255)            gTTCATCGATACCGGTACCCGCTTTCGCAGATACGCGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTc                                                                                                                          <  2:539026/140‑1 (MQ=255)                    aTACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAac                                                                                                                  <  1:1452570/140‑1 (MQ=255)                     tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAacc                                                                                                                 <  1:281628/140‑1 (MQ=255)                      aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAacca                                                                                                                <  1:526972/140‑1 (MQ=255)                      aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAacca                                                                                                                <  1:1452866/140‑1 (MQ=255)                       ccGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTg                                                                                                                      <  1:609070/133‑1 (MQ=255)                       ccGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTg                                                                                                                      >  2:609070/1‑133 (MQ=255)                       ccGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAaccac                                                                                                               >  1:212459/1‑140 (MQ=255)                       ccGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAacca                                                                                                                >  1:1009989/1‑139 (MQ=255)                       ccGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAacca                                                                                                                <  2:1009989/139‑1 (MQ=255)                        cGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAaccacc                                                                                                              <  2:654889/140‑1 (MQ=255)                          gTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAaccaccac                                                                                                            >  1:367301/1‑140 (MQ=255)                                                     aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTg                                                                                 >  1:251830/1‑140 (MQ=255)                                                      aCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTgg                                                                                >  1:1029974/1‑140 (MQ=255)                                                        tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGAt                                                                              <  2:251830/140‑1 (MQ=255)                                                          gCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGAt                                                                              <  1:103857/138‑1 (MQ=255)                                                          gCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGAt                                                                              >  2:103857/1‑138 (MQ=255)                                                               cACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCg                                                                       >  1:104368/1‑140 (MQ=255)                                                                aCCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGa                                                                      <  1:1530265/140‑1 (MQ=255)                                                                aCCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGa                                                                      <  1:812554/140‑1 (MQ=255)                                                                   gCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATgg                                                                   <  2:790503/140‑1 (MQ=255)                                                                    ccccACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGt                                                                  >  1:638820/1‑140 (MQ=255)                                                                       cACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTg                                                               <  2:1043304/140‑1 (MQ=255)                                                                          tctTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTAGCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGctg                                                            >  2:1419523/1‑138 (MQ=255)                                                                          tctTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCgg                                                            <  1:616496/140‑1 (MQ=255)                                                                            ttCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCa                                                          <  2:1029974/140‑1 (MQ=255)                                                                            ttCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCa                                                          >  2:581040/1‑140 (MQ=255)                                                                            ttCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCa                                                          <  1:581040/140‑1 (MQ=255)                                                                              ccGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATc                                                        >  2:236182/1‑140 (MQ=255)                                                                                    ggATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACcg                                                  >  1:1188059/1‑140 (MQ=255)                                                                                            tACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGgc                                          >  1:918698/1‑140 (MQ=255)                                                                                                   agagTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGcaaca                                   >  1:504783/1‑140 (MQ=255)                                                                                                      gttcgttcTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAAcc                                >  1:1500160/1‑140 (MQ=255)                                                                                                      gttcgttcTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAAcc                                >  1:1402546/1‑140 (MQ=255)                                                                                                                aaCGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGa                      <  2:212459/140‑1 (MQ=255)                                                                                                                 aCGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGAt                     >  1:228151/1‑140 (MQ=255)                                                                                                                            aTCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCt          >  1:19182/1‑140 (MQ=255)                                                                                                                             tCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTg         <  2:1500160/140‑1 (MQ=255)                                                                                                                                 cTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGca     >  1:236655/1‑140 (MQ=255)                                                                                                                                 cTTCTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGca     >  1:141999/1‑140 (MQ=255)                                                                                                                                    cTGGCTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGcacca  <  2:1402546/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCGCGTGCTGGATTGCTTCGATGGTCTGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCA  >  NC_000913/3314374‑3314644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑