Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,445	A→G	S97S (AGT→AGC)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,445	0	A	G	100.0%	340.2 / NA	98	S97S (AGT→AGC)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/48);  total (50/48)

AAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATG  >  NC_000913/3332309‑3332580                                                                                                                                         |                                                                                                                                        aaaaCATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTc                                                                                                                                      <  2:598707/140‑1 (MQ=255) aaaaCATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTc                                                                                                                                      <  2:17909/140‑1 (MQ=255)    aCATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAgg                                                                                                                                   <  1:1209563/140‑1 (MQ=255)    aCATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAgg                                                                                                                                   <  2:522897/140‑1 (MQ=255)     cATCACCAGGCCGCTCAGGAGCATCAATGTCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGt                                                                                                                                  >  1:1399474/1‑140 (MQ=255)     cATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGt                                                                                                                                  >  2:325407/1‑140 (MQ=255)       tCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTAt                                                                                                                                >  2:1408630/1‑140 (MQ=255)         aCCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTg                                                                                                                              <  1:297690/140‑1 (MQ=255)         aCCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTg                                                                                                                              <  1:962146/140‑1 (MQ=255)           cAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCa                                                                                                                            <  2:758548/140‑1 (MQ=255)              gCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAgg                                                                                                                         <  1:346088/140‑1 (MQ=255)               ccGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGa                                                                                                                        >  2:1443053/1‑140 (MQ=255)               ccGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGa                                                                                                                        >  2:670014/1‑140 (MQ=255)                  cTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAgg                                                                                                                         <  1:803918/136‑1 (MQ=255)                  cTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAgg                                                                                                                         >  2:803918/1‑136 (MQ=255)                  cTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGa                                                                                                                     >  1:884725/1‑140 (MQ=255)                  cTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGa                                                                                                                     >  2:268425/1‑140 (MQ=255)                     aGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCt                                                                                                                  <  1:1393675/140‑1 (MQ=255)                           aTCAATGTCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAg                                                                                                            <  2:1399474/140‑1 (MQ=255)                           aTCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAg                                                                                                            <  1:1408630/140‑1 (MQ=255)                            tCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAgg                                                                                                                         <  2:1159402/126‑1 (MQ=255)                            tCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAgg                                                                                                                         >  1:1159402/1‑126 (MQ=255)                            tCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAgg                                                                                                           >  2:836379/1‑140 (MQ=255)                            tCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAgg                                                                                                           >  2:1255632/1‑140 (MQ=255)                             cAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGa                                                                                                          <  2:1325344/140‑1 (MQ=255)                               aTGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGTTGGCAACCATCATACCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATg                                                                                                        >  1:826439/1‑140 (MQ=255)                               aTGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCCGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATg                                                                                                        >  2:751012/1‑140 (MQ=255)                                tGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGa                                                                                                       <  2:848667/140‑1 (MQ=255)                                  ccccTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAg                                                                                                     <  1:441132/140‑1 (MQ=255)                                   cccTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGt                                                                                                    >  1:214003/1‑140 (MQ=255)                                    ccTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGtt                                                                                                   >  1:544163/1‑140 (MQ=255)                                        cAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGAGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCa                                                                                               >  2:818715/1‑140 (MQ=255)                                          aCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCaaa                                                                                             <  2:214003/140‑1 (MQ=255)                                          aCCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCaaa                                                                                             <  2:1196560/140‑1 (MQ=255)                                           ccTGAGTGCTGCGCATTTTCTCTTTGAGGAGAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAAc                                                                                            >  2:1368477/1‑140 (MQ=255)                                               aGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGcc                                                                                        >  2:665333/1‑140 (MQ=255)                                               aGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGcc                                                                                        >  2:404258/1‑140 (MQ=255)                                               aGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTACCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGcc                                                                                        >  2:1347403/1‑140 (MQ=255)                                                 tgctgcGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCTAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcg                                                                                      <  1:1218326/140‑1 (MQ=255)                                                  gctgcGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcgg                                                                                     >  2:396122/1‑140 (MQ=255)                                                   ctgcGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcggc                                                                                    >  1:668248/1‑140 (MQ=255)                                                    tgcGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcggcg                                                                                   <  1:458758/140‑1 (MQ=255)                                                    tgcGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcggcg                                                                                   <  1:1443053/140‑1 (MQ=255)                                                     gcgcATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcggcgg                                                                                  >  1:233252/1‑140 (MQ=255)                                                     gcgcATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCcggcgg                                                                                  <  2:233252/140‑1 (MQ=255)                                                      cgcATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGt                                                                                 >  1:487417/1‑140 (MQ=255)                                                      cgcATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGt                                                                                 <  1:836379/140‑1 (MQ=255)                                                      cgcATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGt                                                                                 >  2:615193/1‑140 (MQ=255)                                                         aTTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGc                                                                              <  1:268425/140‑1 (MQ=255)                                                         aTTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGc                                                                              <  2:1494604/140‑1 (MQ=255)                                                            ttCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCAc                                                                           <  1:665333/140‑1 (MQ=255)                                                                  ttGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCcaa                                                                     <  1:1255632/140‑1 (MQ=255)                                                                   tGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCcaac                                                                    >  2:13737/1‑140 (MQ=255)                                                                      ggATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACaaa                                                                 <  2:165934/140‑1 (MQ=255)                                                                           aaTACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCaa                                                            <  2:668248/140‑1 (MQ=255)                                                                               cGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAg                                                        >  1:793426/1‑140 (MQ=255)                                                                                gCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGc                                                       >  1:657143/1‑140 (MQ=255)                                                                                  tGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGt                                                     >  1:197454/1‑140 (MQ=255)                                                                                   ggCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTg                                                    >  1:890281/1‑140 (MQ=255)                                                                                   ggCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCCGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTg                                                    <  1:751012/140‑1 (MQ=255)                                                                                    gCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTgg                                                   >  2:869226/1‑140 (MQ=255)                                                                                    gCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTgg                                                   <  1:1347403/140‑1 (MQ=255)                                                                                    gCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTgg                                                   >  1:1338736/1‑140 (MQ=255)                                                                                     cAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGc                                                  <  2:1095720/140‑1 (MQ=255)                                                                                        ccATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTa                                               <  1:869226/140‑1 (MQ=255)                                                                                           tcatGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGa                                            <  1:325407/140‑1 (MQ=255)                                                                                              tGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAac                                         >  2:1468274/1‑140 (MQ=255)                                                                                              tGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAac                                         >  2:248015/1‑140 (MQ=255)                                                                                              tGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAac                                         <  2:657143/140‑1 (MQ=255)                                                                                              tGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAac                                         >  2:626560/1‑140 (MQ=255)                                                                                               gCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAaca                                        <  1:13737/140‑1 (MQ=255)                                                                                                ccAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAacac                                       >  1:868069/1‑140 (MQ=255)                                                                                                 cAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACAcg                                      <  2:884725/140‑1 (MQ=255)                                                                                                    cTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGt                                   >  2:1169159/1‑140 (MQ=255)                                                                                                    cTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGt                                   >  1:234659/1‑140 (MQ=255)                                                                                                       gCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAAt                                >  1:1479706/1‑140 (MQ=255)                                                                                                        cGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATg                               <  1:248015/140‑1 (MQ=255)                                                                                                        cGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGACGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATg                               >  1:1148581/1‑140 (MQ=255)                                                                                                         gagaGTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATgg                              >  1:45829/1‑140 (MQ=255)                                                                                                          agagTTGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATggc                             >  2:877124/1‑140 (MQ=255)                                                                                                              ttGCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCa                         <  2:1399448/140‑1 (MQ=255)                                                                                                                gCCCAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAAc                       <  1:1468274/140‑1 (MQ=255)                                                                                                                 cccAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCa                         <  2:1362006/137‑1 (MQ=255)                                                                                                                 cccAATCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCa                         >  1:1362006/1‑137 (MQ=255)                                                                                                                      tCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCtcttc                 >  1:417584/1‑140 (MQ=255)                                                                                                                      tCACCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCtcttc                 >  2:59679/1‑140 (MQ=255)                                                                                                                        aCCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTcttctt               <  1:670014/140‑1 (MQ=255)                                                                                                                        aCCTGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTcttctt               <  2:1124975/140‑1 (MQ=255)                                                                                                                           tGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACGAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCAc            >  1:525081/1‑140 (MQ=255)                                                                                                                           tGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCAc            >  1:546180/1‑140 (MQ=255)                                                                                                                           tGCGAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCAc            <  2:868069/140‑1 (MQ=255)                                                                                                                              gAAGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGc         <  1:404258/140‑1 (MQ=255)                                                                                                                                aGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCaa       <  1:59679/140‑1 (MQ=255)                                                                                                                                aGCGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCaa       <  2:45829/140‑1 (MQ=255)                                                                                                                                  cGGTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGa     >  1:455542/1‑140 (MQ=255)                                                                                                                                    gTCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAAt   >  2:1196663/1‑140 (MQ=255)                                                                                                                                    gTCGGGCTCAGGAATTGCAAGGATGAGCTGAACAGGATGAAGATCCCAAACAGCCCGGCGGTCGGCACTGGCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCCAACTATTCTTCACAGCAAGAAt   >  2:89785/1‑140 (MQ=255)                                                                                                                                     tCGGGCTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATg  <  2:417584/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCGCATTTTCTCTTTGAGGATAAATACGCTGGCAACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATG  >  NC_000913/3332309‑3332580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑