Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,287	(T)5→4	coding (440/591 nt)	uhpA ←	DNA‑binding transcriptional activator UhpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,283	0	T	.	100.0%	378.6 / NA	84	coding (444/591 nt)	uhpA	DNA‑binding transcriptional activator UhpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (46/38);  total (46/38)

AACATGCGGCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTA  >  NC_000913/3850149‑3850418                                                                                                                                       |                                                                                                                                        accATGCGGCGCGCCGGCTTTACGTCTTTACTGACGCCAAGTTTTTCCATCAGTTGGGCGCGATGGCCGTGTACCGTTTTCGGTGACAAGCCCAGTTGGGCGGCAATCTTTTTCACCGCCATTCCTTGCGCCAGttttcc                                                                                                                                    <  2:1332571/138‑3 (MQ=255) aaCATGCGGCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTCCCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttttcc                                                                                                                                    <  1:59946/140‑3 (MQ=255) aaCATGCGGCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttttcc                                                                                                                                    <  1:1533636/140‑3 (MQ=255) aaCATGCGGCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttttcc                                                                                                                                    <  1:1394509/140‑3 (MQ=255)      gcggcgCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCAc                                                                                                                              >  1:238481/1‑140 (MQ=255)      gcggcgCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCAc                                                                                                                              >  1:1528728/1‑140 (MQ=255)       cggcgCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATGGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTCCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCAcc                                                                                                                             <  1:973667/140‑1 (MQ=255)            cgcCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCg                                                                                                                        >  1:224073/1‑140 (MQ=255)            cgcCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCg                                                                                                                        >  1:105750/1‑140 (MQ=255)              ccAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGtt                                                                                                                      <  1:620738/140‑1 (MQ=255)              ccAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGtt                                                                                                                      >  1:1146251/1‑140 (MQ=255)              ccAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGtt                                                                                                                      <  2:1534028/140‑1 (MQ=255)                    ctACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGtt                                                                                                                <  1:1071685/140‑1 (MQ=255)                    ctACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGtt                                                                                                                >  1:348322/1‑140 (MQ=255)                       cgtcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTgg                                                                                                             <  1:1320932/140‑1 (MQ=255)                       cgtcgtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTgg                                                                                                             >  1:504972/1‑140 (MQ=255)                           gtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAg                                                                                                         >  2:439077/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  2:488480/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  1:1452295/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  2:176879/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  1:433662/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  2:293511/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  2:1250772/1‑140 (MQ=255)                            ttACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                                                                                                        >  2:921996/1‑140 (MQ=255)                             tACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCg                                                                                                       >  1:707911/1‑140 (MQ=255)                             tACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCg                                                                                                       >  1:1167368/1‑140 (MQ=255)                               cTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGA‑AAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGt                                                                                                    >  2:565609/1‑140 (MQ=255)                                  aCGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTcc                                                                                                  >  1:86664/1‑140 (MQ=255)                                  aCGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTcc                                                                                                  >  1:48736/1‑140 (MQ=255)                                    gCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTg                                                                                                >  1:1495494/1‑140 (MQ=255)                                      ccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgac                                                                                              >  1:1464400/1‑140 (MQ=255)                                         gTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac                                                                                           <  1:1333926/140‑1 (MQ=255)                                           ttttCCATCAGATTGGCGCGAGGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGAAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCg                                                                                         <  2:35381/140‑1 (MQ=255)                                                   cAGATTGGCGCGATGGACGTGTCCCGTTTTCGGTGACAAGCCCAGTTCGGCGGCATTCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAg                                                                                 <  1:1121046/140‑1 (MQ=255)                                                    aGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGt                                                                                >  1:332641/1‑140 (MQ=255)                                                    aGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGt                                                                                >  1:341337/1‑140 (MQ=255)                                                     gATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGtt                                                                               >  1:1147335/1‑140 (MQ=255)                                                         ggCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAAt                                                                           >  2:842342/1‑140 (MQ=255)                                                             cgATGGCCGTGTCCCGTTTCGGGTGACAAGCCCAGTTCGGCGGCATTCTCTTTCCCCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCa                                                                       <  2:1446509/140‑1 (MQ=255)                                                                  gACGTGTCCCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATc                                                                  <  2:903170/140‑1 (MQ=255)                                                                  gACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATc                                                                  <  1:254260/140‑1 (MQ=255)                                                                    cGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCg                                                                >  2:415055/1‑140 (MQ=255)                                                                        gccctttttCGTTGACACGCCCTGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGTCCGGATGCCAGTTTAATGGCAATATCCGGCGt                                                            <  2:49421/135‑1 (MQ=255)                                                                          ccGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCC‑GTTTAATGGCAATATCCGGCGTCAg                                                         >  1:476464/1‑140 (MQ=255)                                                                            gTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGa                                                        >  1:742317/1‑140 (MQ=255)                                                                              tttGGGTGACAGGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATa                                                      <  1:1153186/140‑1 (MQ=255)                                                                                  ggTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAg                                                  >  2:105329/1‑140 (MQ=255)                                                                                     gACAAGCCCGGTTCGGCGGCATTCTCTTTCCCCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAgccg                                               <  2:741027/140‑1 (MQ=255)                                                                                     gACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAgccg                                               <  2:1526218/140‑1 (MQ=255)                                                                                     gACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAgccg                                               >  2:1184507/1‑140 (MQ=255)                                                                                       cAAGCCCGGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAgccgcc                                             <  1:535165/140‑1 (MQ=255)                                                                                          gCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGt                                          >  1:796290/1‑140 (MQ=255)                                                                                            ccggTTCGGCGGCATTCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  2:583108/137‑1 (MQ=255)                                                                                            ccggTTCGGCGGCAACCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  2:1320413/137‑1 (MQ=255)                                                                                            ccAGTTGGGCGGCACTCTCTTCCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  2:224073/140‑1 (MQ=255)                                                                                            ccAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  1:439077/140‑1 (MQ=255)                                                                                            ccAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  1:921996/140‑1 (MQ=255)                                                                                            ccAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  2:1528728/140‑1 (MQ=255)                                                                                            caggtgcggcggCAACCTCTTTCTCCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTgg                                        <  2:105750/134‑1 (MQ=255)                                                                                             cggTTCGGCGGCATTCTTTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGc                                       <  2:1495494/138‑1 (MQ=255)                                                                                              aGTTCGGCGGCATTCTTTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCa                                      <  2:1439330/140‑1 (MQ=255)                                                                                              aGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCa                                      <  2:341337/140‑1 (MQ=255)                                                                                              aGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCa                                      <  1:293511/140‑1 (MQ=255)                                                                                                   ggcggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGt                                 >  1:1002184/1‑140 (MQ=255)                                                                                                    gcggcATTCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTa                                <  2:921607/140‑1 (MQ=255)                                                                                                    gcggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTa                                >  2:818715/1‑140 (MQ=255)                                                                                                    gcggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTa                                >  2:517416/1‑140 (MQ=255)                                                                                                     cggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTAt                               >  1:195283/1‑140 (MQ=255)                                                                                                     cggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTAt                               >  2:493679/1‑140 (MQ=255)                                                                                                      ggcAATCTCTTTCGCCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATg                              <  2:1398888/140‑1 (MQ=255)                                                                                                      ggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATg                              <  2:332641/140‑1 (MQ=255)                                                                                                      ggcAATCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATg                              <  2:707911/140‑1 (MQ=255)                                                                                                          ttctcTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCAcc                          <  2:801511/139‑1 (MQ=255)                                                                                                          aTCTCTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCAcc                          >  2:1097278/1‑140 (MQ=255)                                                                                                           tctcTTTCACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTAACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCg                         <  1:697636/140‑1 (MQ=255)                                                                                                            ccctggtaCCGCCATTCCTTGCGCCAG‑CTTTCCGCCCCCTGGCGTACACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCgc                        <  2:433662/133‑1 (MQ=255)                                                                                                                  cACCGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAAt                  >  1:1500225/1‑140 (MQ=255)                                                                                                                     cGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAg               >  1:24258/1‑140 (MQ=255)                                                                                                                     cGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAg               >  2:40572/1‑140 (MQ=255)                                                                                                                     cGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAg               >  1:362654/1‑140 (MQ=255)                                                                                                                     cGCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAg               <  1:684340/140‑1 (MQ=255)                                                                                                                      gCCATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGt              <  1:1368678/140‑1 (MQ=255)                                                                                                                        cATTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTc            <  2:1146251/140‑1 (MQ=255)                                                                                                                         aTTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCa           >  2:690239/1‑140 (MQ=255)                                                                                                                         aTTCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCa           >  1:1092953/1‑140 (MQ=255)                                                                                                                          ttCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCAt          <  1:176879/140‑1 (MQ=255)                                                                                                                          ttCCTTGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCAt          <  1:105329/140‑1 (MQ=255)                                                                                                                               tGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCggg     >  1:112171/1‑140 (MQ=255)                                                                                                                               tGCGCCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCggg     >  1:1057877/1‑140 (MQ=255)                                                                                                                                  gcCAG‑TTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTa  >  1:675191/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AACATGCGGCGCGCCAGCTCTACGTCGTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTA  >  NC_000913/3850149‑3850418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑