Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	985,846	(A)8→7	intergenic (‑137/+48)	aspC ← / ← ompF	aspartate aminotransferase/outer membrane porin F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	985,839	0	A	.	100.0%	346.5 / NA	77	intergenic (‑130/+55)	aspC/ompF	aspartate aminotransferase/outer membrane porin F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (44/33);  total (44/33)

CTCAAACATGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATAAAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCTGGTTG  >  NC_000913/985701‑985972                                                                                                                                           |                                                                                                                                      ctcAAACATGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATAGCTCTGGAAGGTATaa                                                                                                                                      >  2:1520737/1‑140 (MQ=255) ctcAAACATGACGAGGTTCCATTATGGTTACAGAAAGGAAGTCCGCTATCAAGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTTCAAGACGTGAGATTGCTCTGGAAGGTATaa                                                                                                                                      >  1:915742/1‑140 (MQ=255)    aaaCATGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaa                                                                                                                                   >  2:341321/1‑140 (MQ=255)        aTGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaaca                                                                                                                               <  1:1032651/140‑3 (MQ=255)         tGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaacag                                                                                                                              >  1:169060/1‑137 (MQ=255)         tGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaacag                                                                                                                              >  1:335673/1‑137 (MQ=255)         tGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATaaaaaaacag                                                                                                                              >  1:848760/1‑137 (MQ=255)            cGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGAc                                                                                                                          >  1:1246407/1‑140 (MQ=255)              aGGTTCCATTATGGTTACAGAAGGGAAGCCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCa                                                                                                                        >  1:1355963/1‑140 (MQ=255)                gTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCaaa                                                                                                                      >  2:859759/1‑140 (MQ=255)                gTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCaaa                                                                                                                      >  1:528918/1‑140 (MQ=255)                       tATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGt                                                                                                               <  1:1497372/140‑1 (MQ=255)                          ggTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGtttt                                                                                                            >  1:1456093/1‑140 (MQ=255)                           gTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt                                                                                                           >  2:437543/1‑140 (MQ=255)                           gTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGttttt                                                                                                           >  1:1403098/1‑140 (MQ=255)                              aCAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCg                                                                                                        >  1:151405/1‑140 (MQ=255)                                aGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGc                                                                                                      <  1:1058997/140‑1 (MQ=255)                                 gAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCa                                                                                                     <  2:1458246/140‑1 (MQ=255)                                  aaGGGAAGTCCGCTATCAGGGTAACGGGGGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTG‑TTTTTCGGCAtt                                                                                                   <  1:709994/140‑1 (MQ=255)                                   aGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAtt                                                                                                   >  2:153213/1‑140 (MQ=255)                                    gggAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAttt                                                                                                  >  2:481808/1‑140 (MQ=255)                                    gggAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCAttt                                                                                                  >  1:680813/1‑140 (MQ=255)                                     ggAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTa                                                                                                 <  1:1555776/140‑1 (MQ=255)                                     ggAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTa                                                                                                 >  2:960124/1‑140 (MQ=255)                                     ggAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTa                                                                                                 >  2:776306/1‑140 (MQ=255)                                      gAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTaa                                                                                                >  1:980453/1‑140 (MQ=255)                                       aaGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAAc                                                                                               >  1:1331127/1‑140 (MQ=255)                                         gTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACaa                                                                                             >  2:634989/1‑140 (MQ=255)                                         gTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACaa                                                                                             <  1:341321/140‑1 (MQ=255)                                            cGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAaga                                                                                          >  1:1246241/1‑140 (MQ=255)                                                aTCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGgtg                                                                                      <  1:409256/140‑1 (MQ=255)                                                 tCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGgtgt                                                                                     <  2:176927/140‑1 (MQ=255)                                                 tCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGgtgt                                                                                     <  1:1088540/140‑1 (MQ=255)                                                  cAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGtgtg                                                                                    <  1:36349/140‑1 (MQ=255)                                                    gggTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCt                                                                                  >  1:41033/1‑140 (MQ=255)                                                      gTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAt                                                                                >  1:1106732/1‑140 (MQ=255)                                                      gTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTAt                                                                                <  1:243452/140‑1 (MQ=255)                                                        aaCGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTa                                                                              >  2:171281/1‑140 (MQ=255)                                                         aCGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAg                                                                             >  2:1433561/1‑140 (MQ=255)                                                          cGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGa                                                                            >  1:250997/1‑140 (MQ=255)                                                            ggAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAAc                                                                          <  1:404052/140‑1 (MQ=255)                                                             gagaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACt                                                                         >  2:1325820/1‑140 (MQ=255)                                                               gaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTgg                                                                       >  2:763590/1‑140 (MQ=255)                                                               gaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTgg                                                                       >  1:1351541/1‑140 (MQ=255)                                                               gaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTgg                                                                       >  2:1075864/1‑140 (MQ=255)                                                               gaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTgg                                                                       >  1:787406/1‑140 (MQ=255)                                                               gaTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTgg                                                                       >  1:817407/1‑140 (MQ=255)                                                                aTTTACCAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGt                                                                      <  2:1155024/140‑1 (MQ=255)                                                                aTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGt                                                                      <  1:358658/140‑1 (MQ=255)                                                                aTTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGt                                                                      >  1:1255726/1‑140 (MQ=255)                                                                 tttACAAAATTCCGACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTa                                                                     >  2:1509786/1‑140 (MQ=255)                                                                 tttACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTa                                                                     >  2:382644/1‑140 (MQ=255)                                                                  ttACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa                                                                    >  1:322885/1‑140 (MQ=255)                                                                  ttACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaa                                                                    >  1:1533654/1‑140 (MQ=255)                                                                   tACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTaaa                                                                   <  1:153213/140‑1 (MQ=255)                                                                     cAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACg                                                                 >  2:1001881/1‑140 (MQ=255)                                                                     cAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACg                                                                 <  2:1351541/140‑1 (MQ=255)                                                                          ttCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATAcc                                                            <  2:200462/140‑1 (MQ=255)                                                                          ttCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATAcc                                                            <  2:528918/140‑1 (MQ=255)                                                                          ttCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATAcc                                                            <  1:634989/140‑1 (MQ=255)                                                                          ttCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATAcc                                                            >  1:584141/1‑140 (MQ=255)                                                                                    tACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACg                                                  >  1:1015557/1‑140 (MQ=255)                                                                                       tgatgaAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGgtg                                               <  1:171281/140‑1 (MQ=255)                                                                                       tgatgaAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGgtg                                               <  1:1493437/140‑1 (MQ=255)                                                                                          tgaAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTgtcg                                            >  1:404029/1‑140 (MQ=255)                                                                                           gaAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTgtcgt                                           >  1:1439705/1‑140 (MQ=255)                                                                                              aaCGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTg                                        >  1:1434450/1‑140 (MQ=255)                                                                                              aaCGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTg                                        <  1:161066/140‑1 (MQ=255)                                                                                                   aGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCt                                   >  2:1345595/1‑140 (MQ=255)                                                                                                    ggCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTa                                  >  2:1593759/1‑140 (MQ=255)                                                                                                         tttttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGccc                             <  1:1325820/140‑1 (MQ=255)                                                                                                         tttttGCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGccc                             <  2:798752/140‑1 (MQ=255)                                                                                                         tttttGCAAGACGTGAGATAGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGccc                             <  1:1520737/140‑1 (MQ=255)                                                                                                              gCAAGACGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGttt                        <  2:420584/140‑1 (MQ=255)                                                                                                                   aCGTGAGATTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTtgttgt                   <  1:776306/140‑1 (MQ=255)                                                                                                                         gaTTGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAAt             <  2:787406/140‑1 (MQ=255)                                                                                                                            tGCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGa          >  2:1584330/1‑140 (MQ=255)                                                                                                                             gCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGAt         <  1:763590/140‑1 (MQ=255)                                                                                                                             gCTCTGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGAt         <  2:481686/140‑1 (MQ=255)                                                                                                                              ctctGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATc        <  1:1444429/140‑1 (MQ=255)                                                                                                                               tctGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCt       >  2:489061/1‑140 (MQ=255)                                                                                                                                ctGGAAGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCTg      >  1:580551/1‑140 (MQ=255)                                                                                                                                    aaGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCTGGTtg  <  2:1255726/140‑1 (MQ=255)                                                                                                                                    aaGGTAT‑AAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCTGGTtg  <  2:1434450/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      CTCAAACATGACGAGGTTCCATTATGGTTACAGAAGGGAAGTCCGCTATCAGGGTAACGGGAGATTTACAAAATTCCAACTATTACTGATGAAAACGCAGGCTGTTTTTGCAAGACGTGAGATTGCTCTGGAAGGTATAAAAAAAACAGGACCAAAGTCCTGTTTTTTCGGCATTTAACAAAGAGGTGTGCTATTAGAACTGGTAAACGATACCCACAGCAACGGTGTCGTCTGAACCTACGCCCAGTTTGTTGTCAGAATCGATCTGGTTG  >  NC_000913/985701‑985972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑