Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,173	0	T	G	28.1%	141.5 / 22.8	65	G31G (GGT→GGG)	ymdF	conserved protein YmdF
Reads supporting (aligned to +/- strand):  ref base T (17/29);  new base G (18/0);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTTAATTCTATGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCG  >  NC_000913/1068034‑1068312                                                                                                                                            |                                                                                                                                            cgTTAATTCTATGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGt                                                                                                                                             <  1:834719/140‑1 (MQ=255) cgTTAATTCTATGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGt                                                                                                                                             <  2:840696/140‑1 (MQ=255)    tAATTCTATGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAAt                                                                                                                                          <  2:794254/140‑1 (MQ=255)           aTGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAACGGGGGGAATTTCAAGa                                                                                                                                   >  1:1363360/1‑140 (MQ=255)            tGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAAGTGGACAGCACAGCGGGGGGAATTTCAAGaa                                                                                                                                  >  2:556192/1‑140 (MQ=255)            tGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAAAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAGaa                                                                                                                                  >  2:1380618/1‑140 (MQ=255)                atatGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGAc                                                                                                                              >  1:140270/1‑140 (MQ=255)                 tatGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAAAGCAACAGAAGCAGGTAAAAAAGGGGGACAGCACAGCGGGGGGAAATTCAAGAATGAcc                                                                                                                             >  2:524338/1‑140 (MQ=255)                         cTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgcgc                                                                                                                     <  2:504578/140‑1 (MQ=255)                         cTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgcgc                                                                                                                     <  2:531565/140‑1 (MQ=255)                         cTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAAAGACCCGCCgcgc                                                                                                                     >  1:443371/1‑140 (MQ=255)                           ataCCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAgcgcgc                                                                                                                   >  1:267720/1‑140 (MQ=255)                           ataCCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAAAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAgcgcgc                                                                                                                   >  2:1216419/1‑140 (MQ=255)                           ataCCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAAATTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAgcgcgc                                                                                                                   >  2:738476/1‑140 (MQ=255)                             aCCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCCCAGCGCGCAt                                                                                                                 >  2:1500764/1‑140 (MQ=255)                             aCCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCAt                                                                                                                 >  1:1292507/1‑140 (MQ=255)                                cAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGGCAGCACAGCGGGGGGAATTTCAAGAAAGACCCGCAGCGCGCATCTg                                                                                                              >  2:1440183/1‑140 (MQ=255)                                 aGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa                                                                                                             <  2:1035852/140‑1 (MQ=255)                                 aGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGa                                                                                                             <  2:375967/140‑1 (MQ=255)                                  gACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCCATTTTGCAGAAGACCGCGGAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACCGCCGGGGGAATTTCAAGAAAGAACCCCCGCGCGCAACTGaa                                                                                                            >  2:412248/1‑140 (MQ=255)                                  gACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGaa                                                                                                            >  1:998471/1‑140 (MQ=255)                                   aCGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAg                                                                                                           <  1:1202990/140‑1 (MQ=255)                                   aCGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAg                                                                                                           <  1:1175070/140‑1 (MQ=255)                                    cGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGc                                                                                                          <  2:904201/140‑1 (MQ=255)                                    cGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGc                                                                                                          >  2:1157730/1‑140 (MQ=255)                                      gAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAg                                                                                                        >  2:1039479/1‑140 (MQ=255)                                        ggTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAggg                                                                                                      >  1:520968/1‑139 (MQ=255)                                         gTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTa                                                                                                     <  2:124156/140‑1 (MQ=255)                                         gTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCCCAGCGCGCATCTGAAGCAggga                                                                                                     >  2:953894/1‑138 (MQ=255)                                          tCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaa                                                                                                    <  1:496870/140‑1 (MQ=255)                                              tAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaa                                                                                                <  1:964556/140‑1 (MQ=255)                                              tAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaa                                                                                                <  2:421075/140‑1 (MQ=255)                                               aaTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAg                                                                                               <  2:1263783/140‑1 (MQ=255)                                                 tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGGAGAAAACCGCCAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGGaaaaaaagg                                                                                             >  2:1363386/1‑137 (MQ=255)                                                   gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAAAGCATCAGAAGCAGGTAAAAAAGGGGGACAGCACCGCGGGGGGAAATTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGGAAAAAAggtgg                                                                                           >  1:157292/1‑140 (MQ=255)                                                    cAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAggtggt                                                                                          <  1:889544/140‑1 (MQ=255)                                                    cAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAggtggt                                                                                          <  1:1179742/140‑1 (MQ=255)                                                    cAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGGGGACAGCACAGCGGGGGGAATTTTAAAAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAagtggg                                                                                          >  1:826785/1‑139 (MQ=255)                                                        ccATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGGAAAAAAGGTGGACAGCACAGCGGGGGGAATTTCAAAAATGACCCGCAGCGCGCATCTGAAGCAGGGAAAAAAGGGGGTAaga                                                                                      >  1:1504463/1‑140 (MQ=255)                                                         cATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGATAAAAAAGGTGGTAagag                                                                                     <  1:1054016/140‑1 (MQ=255)                                                                              tttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                                >  2:1261465/1‑140 (MQ=255)                                                                              tttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                                <  2:1313701/140‑1 (MQ=255)                                                                              tttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                                <  1:193848/140‑1 (MQ=255)                                                                                  cAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAg                                                            <  1:953894/140‑1 (MQ=255)                                                                                  cAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAg                                                            <  1:314085/140‑1 (MQ=255)                                                                                   agaagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGc                                                           <  1:1039479/140‑1 (MQ=255)                                                                                    gaagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                                          <  1:1500764/140‑1 (MQ=255)                                                                                      agaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCACGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCgg                                                        <  2:124887/140‑1 (MQ=255)                                                                                       gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                       <  1:1382761/140‑1 (MQ=255)                                                                                        aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                                      <  2:1321268/140‑1 (MQ=255)                                                                                          cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                    >  2:1010727/1‑140 (MQ=255)                                                                                          cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                    >  2:1363004/1‑140 (MQ=255)                                                                                           gcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTaa                                                   <  2:998471/140‑1 (MQ=255)                                                                                            cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                                  >  2:174890/1‑140 (MQ=255)                                                                                            cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                                  >  1:916836/1‑140 (MQ=255)                                                                                            cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                                  >  2:1505524/1‑140 (MQ=255)                                                                                                      tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGaa                                        >  2:194379/1‑140 (MQ=255)                                                                                                       cAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAt                                       >  2:623827/1‑140 (MQ=255)                                                                                                            gCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtt                                  >  1:38375/1‑140 (MQ=255)                                                                                                            gCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtt                                  <  2:520968/140‑1 (MQ=255)                                                                                                                 tAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGt                             >  1:178386/1‑140 (MQ=255)                                                                                                                 tAAAAAAGGTGGACAGCACAGCGGGGGAAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGt                             >  1:978711/1‑140 (MQ=255)                                                                                                                    aaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTg                          <  2:157292/140‑1 (MQ=255)                                                                                                                     aaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGt                         <  2:632596/140‑1 (MQ=255)                                                                                                                          tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTc                    >  1:631964/1‑140 (MQ=255)                                                                                                                          tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTc                    >  1:1055190/1‑140 (MQ=255)                                                                                                                           ggACAGCACAGCGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCg                   >  1:1424596/1‑140 (MQ=255)                                                                                                                               agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGAGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa               >  2:217378/1‑140 (MQ=255)                                                                                                                                   cagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAg           >  2:83624/1‑140 (MQ=255)                                                                                                                                      cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCa        >  1:1426945/1‑140 (MQ=255)                                                                                                                                      cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCa        >  2:881265/1‑140 (MQ=255)                                                                                                                                           gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc   <  1:1363004/140‑1 (MQ=255)                                                                                                                                           gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc   >  2:857535/1‑140 (MQ=255)                                                                                                                                            tAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGACAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccg  <  1:235755/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            CGTTAATTCTATGCAATATGATGTCTATACCCAGACGGAGGTCAGTAATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCG  >  NC_000913/1068034‑1068312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑