Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440TA28.6% 59.9 / 11.1 35intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (2/23);  new base A (10/0);  total (12/23)
Fisher's exact test for biased strand distribution p-value = 3.60e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206315‑3206583
                                                                                                                                   |                                                                                                                                           
tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATAAAAAAAATg                                                                                                                                   >  2:17337/1‑140 (MQ=255)
  ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAATAAAAAAAATggg                                                                                                                                 >  1:764387/1‑139 (MQ=255)
   tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                <  1:94204/140‑1 (MQ=255)
   tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                <  1:1474176/140‑1 (MQ=255)
       gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAAAAAAAAATTGAGTTGc                                                                                                                            >  1:218348/1‑140 (MQ=255)
       gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATACAAAAAATTGAGGTgg                                                                                                                            >  2:1250714/1‑139 (MQ=255)
            atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCTACAAATACAAAAACTAGAGTTGCCaata                                                                                                                       >  1:1246035/1‑137 (MQ=255)
              attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAA‑‑ATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                   <  2:550556/140‑1 (MQ=255)
                 cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCAGACAAAAACAAAAACTGGGGTTGCCCTGATGAgg                                                                                                                  >  1:791439/1‑139 (MQ=255)
                   ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                <  2:607052/140‑1 (MQ=255)
                        aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                           <  2:316448/140‑1 (MQ=255)
                        aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                           <  2:145201/140‑1 (MQ=255)
                            tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                       <  1:56781/140‑1 (MQ=255)
                              gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGGAAGaaaaaaaa                                                                                                     >  1:124441/1‑138 (MQ=255)
                                 ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                  <  1:273705/140‑1 (MQ=255)
                                  tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGACAAAAACAAAAAATGGAGATGCCCTGATAAGGGAAAGGAAAAAacaaca                                                                                                 >  1:262869/1‑140 (MQ=255)
                                    aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                               <  2:461702/140‑1 (MQ=255)
                                     ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                              <  2:1334230/140‑1 (MQ=255)
                                      tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                             <  1:160011/140‑1 (MQ=255)
                                           aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                        <  1:1558849/140‑1 (MQ=255)
                                              tGTTAATTACATATTCACGGTGGCAAAAAATATAGAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                     <  1:1175523/140‑1 (MQ=255)
                                              tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGACAAAAAAAAAAAATTGAGTTGCCCTGGTGAGGGAAAGAAAAAAACAACAAGCAGAGAATaa                                                                                     >  1:136440/1‑140 (MQ=255)
                                                ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCA‑ACAAAAAAAAAAAAAGGAGTTGCCATGATGAGCGAAAGTAAAAAACAACAAGCAGTGAaaaaat                                                                                   >  1:1460395/1‑138 (MQ=255)
                                                         tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                          <  1:1250714/140‑1 (MQ=255)
                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑G‑GATTTTTTCAGACAAAAAAAAAAAAAAGGGGTGACACGAAGAGGGAAAAGAATAAAAAAAAAAGAGAGAATAAAATGACAgagaa                                                                         >  2:1493036/1‑139 (MQ=255)
                                                               cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCTGACAAAAACAAAAAATGGAGGTGGCCTGGTTAGGGAAAGTAATAAAAAAAAAGGAGTGAAAAAATTGACAGAGATTGttg                                                                    >  2:377726/1‑138 (MQ=255)
                                                                 gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTCC‑GACAAAAAAAAAAAATGGAGGTGGCCTGATGGGCGGAAAGAAAAAACAACAAGCAGGGAAAAAGTTGACAGAGATTGTCGcc                                                                  >  2:346473/1‑139 (MQ=255)
                                                                 gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGAAAAAAAAAAAAAAAGGGGGTGGCCTTGTGAGCGGAAGGAAAAAAAAACAAGGAGTGAAAAAAGTGAAAGAGATTGTCGcc                                                                  >  2:1245048/1‑139 (MQ=255)
                                                                    gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGTGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                               >  2:330973/1‑140 (MQ=255)
                                                                    gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAAAGGGGGTGGCCTGATGAGCGGAAGGAATAAACAAAAAGCAGGGAAAAAGATGACAGAGAATGTCGCTCAc                                                               >  2:641705/1‑140 (MQ=255)
                                                                          aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                         <  2:1328628/140‑1 (MQ=255)
                                                                              aaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑A‑AATTTTTCCAAACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                     <  1:17337/139‑1 (MQ=255)
                                                                               aaacCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCAt                                                    <  2:764387/140‑1 (MQ=255)
                                                                                aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GAATTTTTCAGACAAAAAAAAAAAAAGGGGGTGCCATGCAGGGGGGAAGGAAAAAACAACAAGCAGGGAAAAAAGTGGCAGAAAATTTTGCTAACTTTTCCCCCCTg                                                   >  2:1596303/1‑140 (MQ=255)
                                                                                           ttAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                        <  1:140775/140‑1 (MQ=255)
                                                                                            tAGTGGTAGTTTGTGGCGGT‑G‑ATTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                       <  2:124441/140‑1 (MQ=255)
                                                                                            tAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                       <  1:744647/140‑1 (MQ=255)
                                                                                              gTGGTATTTTGTGGGGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGTGTTGCCATGATGAGCAAAAGTAATAAGCACCAGGGAGTGAATAAGTAGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                     <  2:1573480/140‑1 (MQ=255)
                                                                                              gTGGTAGTTTGTGGCGGT‑G‑AATTTTCCCAGACAAATACAAAACCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTACCTTTACCGCCATGATTTCTACCCGAAt                                     <  2:1090155/140‑1 (MQ=255)
                                                                                                ggTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAAAAAAAAAAAAGGGGGTTGCAGGATGAGCGCAAGAAAAAAACAACCAGCAGGGAATAAATTGAAAGAGATTGGTGCTAACTTTACCGCCCCGATTTCTACCCGAAAGc                                   >  2:146683/1‑140 (MQ=255)
                                                                                                ggTAGTTTGTGGCGGT‑G‑AAATTTTTCAGACAAAAAAAAAAAAAGGAGGTGTCCTGGTGAGGGGAAATAAAAAAAAAAAGGGAGTGGAAAAAGTGACAGAGAATGTCGCTAACTTTTCCGCCCTGATTTTTTCCCCaaatc                                   >  2:70813/1‑137 (MQ=255)
                                                                                                      ttGTGGCGGT‑G‑AATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGAGGAGCAAAAGTAAAAAGCAACAGGCAGTGAAAAAGTGGACAGAGATGGTCGCGAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                             <  2:517792/140‑1 (MQ=255)
                                                                                                                 g‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                   <  1:1351620/140‑1 (MQ=255)
                                                                                                                           cAGACAAATAAAAAAATGGGAGTTGCCATGAGGAGCGAAAGTAATAAGCAACAGGCGGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa          <  2:346009/140‑1 (MQ=255)
                                                                                                                            aGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCGGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTAGTGGATAAACTaaaa         <  2:262869/140‑1 (MQ=255)
                                                                                                                            aGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:852445/140‑1 (MQ=255)
                                                                                                                                   tACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:1026041/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                           
TGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206315‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑