Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,997,603	C→T	P141S (CCG→TCG)	yigB →	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,997,603	0	C	T	100.0%	297.0 / NA	87	P141S (CCG→TCG)	yigB	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/37);  total (50/37)

TGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGT  >  NC_000913/3997467‑3997739                                                                                                                                         |                                                                                                                                         tGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCgg                                                                                                                                       >  1:1176985/1‑140 (MQ=255)   cAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAg                                                                                                                                     <  1:68157/140‑1 (MQ=255)   cAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAg                                                                                                                                     <  2:1398712/140‑1 (MQ=255)   cAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAg                                                                                                                                     <  2:1110465/140‑1 (MQ=255)       cgcACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgt                                                                                                                                 <  2:1051907/140‑1 (MQ=255)        gcACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgtt                                                                                                                                <  2:1218604/140‑1 (MQ=255)           cacGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgtttgg                                                                                                                             >  1:337218/1‑140 (MQ=255)           cacGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgtttgg                                                                                                                             >  2:864594/1‑140 (MQ=255)           cacGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgtttgg                                                                                                                             >  1:920025/1‑140 (MQ=255)           cacGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTgtttgg                                                                                                                             >  1:1493354/1‑140 (MQ=255)                agcaATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGtttggtttgg                                                                                                                        >  1:568795/1‑140 (MQ=255)                agcaATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGtttggtttgg                                                                                                                        >  2:1469264/1‑140 (MQ=255)                agcaATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGtttggtttgg                                                                                                                        >  2:1035021/1‑140 (MQ=255)                  caATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTgggg                                                                                                                      >  1:650040/1‑140 (MQ=255)                    aTGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGa                                                                                                                    <  2:172359/140‑1 (MQ=255)                    aTGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGa                                                                                                                    <  1:869964/140‑1 (MQ=255)                        tCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGattat                                                                                                                >  2:1463491/1‑140 (MQ=255)                          aaCTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTAttt                                                                                                              <  2:1085003/140‑1 (MQ=255)                            cTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTg                                                                                                            >  2:683513/1‑140 (MQ=255)                            cTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTg                                                                                                            >  1:52387/1‑140 (MQ=255)                               tGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGt                                                                                                         >  2:147400/1‑140 (MQ=255)                               tGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGt                                                                                                         >  2:863627/1‑140 (MQ=255)                                  cAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTtg                                                                                                      >  1:1011196/1‑140 (MQ=255)                                      tGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGtgct                                                                                                  >  1:42837/1‑140 (MQ=255)                                      tGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGtgct                                                                                                  <  1:346437/140‑1 (MQ=255)                                      tGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGtgct                                                                                                  >  1:1530759/1‑140 (MQ=255)                                      tGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGtgct                                                                                                  <  1:688766/140‑1 (MQ=255)                                         cgcAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTgcg                                                                                               >  1:52137/1‑140 (MQ=255)                                         cgcAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTgcg                                                                                               >  2:1179201/1‑140 (MQ=255)                                           cAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTgcgcg                                                                                             <  1:562578/140‑1 (MQ=255)                                              ccGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTg                                                                                          <  1:1187313/140‑1 (MQ=255)                                                   tCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCg                                                                                     >  2:704769/1‑140 (MQ=255)                                                       cGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACg                                                                                 >  1:907435/1‑140 (MQ=255)                                                        gTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACgg                                                                                <  2:1413833/140‑1 (MQ=255)                                                        gTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACgg                                                                                >  1:820217/1‑140 (MQ=255)                                                           ccGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGgcg                                                                             >  1:1240961/1‑140 (MQ=255)                                                           ccGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGgcg                                                                             <  1:1584409/140‑1 (MQ=255)                                                                  aaaCTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCaaaa                                                                      <  2:545400/140‑1 (MQ=255)                                                                    aCTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAAcc                                                                    >  2:1279015/1‑140 (MQ=255)                                                                         cGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCa                                                               <  2:1141795/140‑1 (MQ=255)                                                                         cGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCa                                                               <  2:86001/140‑1 (MQ=255)                                                                             acCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGa                                                           <  1:588444/140‑1 (MQ=255)                                                                               cTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGata                                                         >  2:524598/1‑140 (MQ=255)                                                                               cTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGata                                                         <  2:1230084/140‑1 (MQ=255)                                                                               cTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGata                                                         >  2:977080/1‑140 (MQ=255)                                                                               cTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGata                                                         >  1:869568/1‑140 (MQ=255)                                                                               cTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGata                                                         >  1:301241/1‑140 (MQ=255)                                                                                 tAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATg                                                       <  1:1179201/140‑1 (MQ=255)                                                                                  aaaaCAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGt                                                      >  2:400067/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  1:444856/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  1:1524548/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  2:1571627/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  1:205687/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  2:1298809/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  1:1406579/1‑140 (MQ=255)                                                                                         cTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACttttt                                               >  1:995123/1‑140 (MQ=255)                                                                                          tGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTg                                              >  1:146991/1‑140 (MQ=255)                                                                                            gCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGc                                            <  2:174730/140‑1 (MQ=255)                                                                                                 gaaATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCgg                                       >  2:228162/1‑140 (MQ=255)                                                                                                         cGCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTc                               >  1:822396/1‑140 (MQ=255)                                                                                                          gCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCa                              >  2:246516/1‑140 (MQ=255)                                                                                                          gCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCa                              <  1:147400/140‑1 (MQ=255)                                                                                                          gCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCa                              <  2:1011196/140‑1 (MQ=255)                                                                                                          gCTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCa                              <  2:1240961/140‑1 (MQ=255)                                                                                                           cTGGTGGCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCaa                             >  2:364617/1‑140 (MQ=255)                                                                                                            tggtggCGATCACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAAc                            >  1:662777/1‑140 (MQ=255)                                                                                                                      cACCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCg                  <  2:568795/140‑1 (MQ=255)                                                                                                                       aCCAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCgg                 <  2:820217/140‑1 (MQ=255)                                                                                                                        ccAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGc                <  1:1035021/140‑1 (MQ=255)                                                                                                                        ccAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGc                >  1:1401029/1‑140 (MQ=255)                                                                                                                         cAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCg               <  1:1328899/140‑1 (MQ=255)                                                                                                                         cAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCg               <  2:419559/140‑1 (MQ=255)                                                                                                                         cAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCg               <  2:1493354/140‑1 (MQ=255)                                                                                                                         cAACGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCg               <  1:524598/140‑1 (MQ=255)                                                                                                                          aaCGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCga              <  1:1571627/140‑1 (MQ=255)                                                                                                                          aaCGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCga              <  2:337218/140‑1 (MQ=255)                                                                                                                          aaCGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCga              <  2:65488/140‑1 (MQ=255)                                                                                                                           aCGGTAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCgag             >  1:472253/1‑140 (MQ=255)                                                                                                                               tAACGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCt         >  2:633982/1‑140 (MQ=255)                                                                                                                                aaCGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCtt        <  2:42837/140‑1 (MQ=255)                                                                                                                                 aCGCCCAGTCGGCGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTa       >  2:961853/1‑140 (MQ=255)                                                                                                                                 aCGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTa       >  1:248539/1‑140 (MQ=255)                                                                                                                                  cGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTAc      <  2:146991/140‑1 (MQ=255)                                                                                                                                  cGCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTAc      <  2:920025/140‑1 (MQ=255)                                                                                                                                   gCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACa     >  2:637345/1‑140 (MQ=255)                                                                                                                                   gCCCAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACa     >  2:189016/1‑140 (MQ=255)                                                                                                                                      cAGTCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGt  >  1:1407362/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAACTCACGACACCTTAAAACAGCTGGCGAAGAAATGGCCGCTGGTGGCGATCACCAACGGTAACGCCCAGCCGGAGCTGTTTGGTTTGGGGGATTATTTTGAGTTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGT  >  NC_000913/3997467‑3997739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑