Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	550,392	A→G	T256A (ACG→GCG)	ylbF →	DUF2877 domain‑containing protein YlbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	550,392	0	A	G	100.0%	351.0 / NA	92	T256A (ACG→GCG)	ylbF	DUF2877 domain‑containing protein YlbF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/40);  total (52/40)

TCAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCC  >  NC_000913/550255‑550528                                                                                                                                          |                                                                                                                                         tCAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCg                                                                                                                                        >  2:823023/1‑140 (MQ=255)  cAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATgcgc                                                                                                                                       <  1:1070881/140‑1 (MQ=255)  cAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATgcgc                                                                                                                                       <  1:417245/140‑1 (MQ=255)  cAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATgcgc                                                                                                                                       >  1:1553330/1‑140 (MQ=255)      tATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCgctgct                                                                                                                                   <  2:109307/140‑1 (MQ=255)      tATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCgctgct                                                                                                                                   <  2:144107/140‑1 (MQ=255)       aTTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGctgctg                                                                                                                                  <  2:932818/140‑1 (MQ=255)       aTTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGctgctg                                                                                                                                  >  1:229013/1‑140 (MQ=255)       aTTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGctgctg                                                                                                                                  <  1:877514/140‑1 (MQ=255)       aTTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGctgctg                                                                                                                                  >  2:1066397/1‑140 (MQ=255)       aTTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGctgctg                                                                                                                                  <  2:418039/140‑1 (MQ=255)              ttATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCt                                                                                                                           >  2:253763/1‑140 (MQ=255)                  gCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCt                                                                                                                       >  2:502209/1‑140 (MQ=255)                  gCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCt                                                                                                                       >  2:665244/1‑140 (MQ=255)                    cgcgCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTg                                                                                                                     <  1:779769/140‑1 (MQ=255)                      cgcAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGTAGATGCGCTGCTGGGGTTCTGGCTTGGc                                                                                                                   >  2:474804/1‑140 (MQ=255)                      cgcAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGc                                                                                                                   >  1:1468189/1‑140 (MQ=255)                      cgcAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGc                                                                                                                   <  2:636950/140‑1 (MQ=255)                      cgcAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGc                                                                                                                   >  1:1072283/1‑140 (MQ=255)                      cgcAAGGATATTTCGCCTCGCCACTCCTGCACATTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGATCTGGCTTGGc                                                                                                                   >  1:1284917/1‑140 (MQ=255)                       gcAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTgggc                                                                                                                  <  1:1284958/140‑3 (MQ=255)                        cAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCca                                                                                                                 <  1:357883/140‑1 (MQ=255)                        cAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCca                                                                                                                 <  1:33190/140‑1 (MQ=255)                          aGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCcaac                                                                                                               <  1:515272/140‑1 (MQ=255)                           ggATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCcaaca                                                                                                              >  2:982692/1‑140 (MQ=255)                             atatTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAat                                                                                                            <  2:21713/140‑1 (MQ=255)                             atatTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAat                                                                                                            <  1:357944/140‑1 (MQ=255)                                tttCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAattat                                                                                                         >  2:183919/1‑140 (MQ=255)                                 ttCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAattatt                                                                                                        >  2:1482749/1‑140 (MQ=255)                                 ttCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAattatt                                                                                                        <  1:1042549/140‑1 (MQ=255)                                     cctcgccACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACaa                                                                                                    >  2:1186582/1‑140 (MQ=255)                                      ctcgccACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAg                                                                                                   >  1:261047/1‑140 (MQ=255)                                       tcgccACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAgg                                                                                                  >  1:1370272/1‑140 (MQ=255)                                         gccACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGaa                                                                                                >  2:1569471/1‑140 (MQ=255)                                          ccACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGaaa                                                                                               <  1:823023/140‑1 (MQ=255)                                              tCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCa                                                                                           <  2:638238/140‑1 (MQ=255)                                                cTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATg                                                                                         >  2:1591879/1‑140 (MQ=255)                                                cTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATg                                                                                         <  2:1145163/140‑1 (MQ=255)                                                  gCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGaa                                                                                       <  1:385028/140‑1 (MQ=255)                                                       ttGTTCATGCTCTGAGTTGCCCGAAGCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAacac                                                                                  <  2:625255/140‑1 (MQ=255)                                                       ttGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAacac                                                                                  <  2:372184/140‑1 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  2:1377273/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  2:1038360/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  1:1585839/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  1:284741/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  1:307827/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 >  2:890604/1‑140 (MQ=255)                                                        tGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACt                                                                                 <  1:831616/140‑1 (MQ=255)                                                          ttCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTgg                                                                               >  2:212320/1‑140 (MQ=255)                                                          ttCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTgg                                                                               >  1:1536981/1‑140 (MQ=255)                                                               gCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTtgtg                                                                          >  2:787377/1‑140 (MQ=255)                                                                ctctGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTgg                                                                         <  2:955916/140‑1 (MQ=255)                                                                  ctGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGct                                                                       >  2:881800/1‑140 (MQ=255)                                                                  ctGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGct                                                                       >  2:280429/1‑140 (MQ=255)                                                                  ctGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGct                                                                       >  2:521239/1‑140 (MQ=255)                                                                    gAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGctct                                                                     >  1:1049113/1‑140 (MQ=255)                                                                        tGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTggg                                                                 >  2:1394561/1‑140 (MQ=255)                                                                         gCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTgggg                                                                >  2:174902/1‑140 (MQ=255)                                                                          cccGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTggggg                                                               >  2:327791/1‑140 (MQ=255)                                                                            cGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCa                                                             >  2:1559262/1‑140 (MQ=255)                                                                            cGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTCCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCa                                                             <  1:829521/140‑1 (MQ=255)                                                                             gAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCaa                                                            <  2:1324305/140‑1 (MQ=255)                                                                               aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACg                                                          >  2:1584969/1‑140 (MQ=255)                                                                               aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACg                                                          >  1:280181/1‑140 (MQ=255)                                                                               aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACg                                                          <  1:1482749/140‑1 (MQ=255)                                                                               aaCGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGACTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACg                                                          >  1:1360097/1‑140 (MQ=255)                                                                                    aCCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTa                                                     >  1:252314/1‑140 (MQ=255)                                                                                    aCCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGACTCTTGGGGGCAACGCCTTa                                                     >  2:1492394/1‑140 (MQ=255)                                                                                      cGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACt                                                   <  2:229013/140‑1 (MQ=255)                                                                                        cTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTcc                                                 >  1:1155448/1‑140 (MQ=255)                                                                                         tGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCa                                                >  2:143873/1‑140 (MQ=255)                                                                                         tGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCa                                                >  1:1422947/1‑140 (MQ=255)                                                                                              cGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGcgcg                                           <  2:1043295/140‑1 (MQ=255)                                                                                                      tCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGgcg                                   <  2:284741/140‑1 (MQ=255)                                                                                                       cGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGgcgc                                  <  1:324606/140‑1 (MQ=255)                                                                                                           gctgGCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGAc                              <  1:1591879/140‑1 (MQ=255)                                                                                                              ggCGCTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGc                           >  1:966093/1‑140 (MQ=255)                                                                                                               gcgcTGGGGCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCa                          >  2:408841/1‑140 (MQ=255)                                                                                                                    ggggCATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGaaaa                     >  1:1284638/1‑140 (MQ=255)                                                                                                                        cATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaa                 <  2:1553330/140‑1 (MQ=255)                                                                                                                        cATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaa                 <  2:19218/140‑1 (MQ=255)                                                                                                                        cATACGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaa                 <  1:183919/140‑1 (MQ=255)                                                                                                                           aCGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatat              <  1:1559262/140‑1 (MQ=255)                                                                                                                            cGTCAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatc             <  1:1394561/140‑1 (MQ=255)                                                                                                                               cAGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgca          >  1:859343/1‑140 (MQ=255)                                                                                                                                aGGGGCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaa         >  2:1341903/1‑140 (MQ=255)                                                                                                                                 ggggCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaat        <  1:160459/140‑1 (MQ=255)                                                                                                                                 ggggCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaat        >  1:1039339/1‑140 (MQ=255)                                                                                                                                  gggCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaata       >  2:614641/1‑140 (MQ=255)                                                                                                                                  gggCAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaata       >  2:1391997/1‑140 (MQ=255)                                                                                                                                     cAGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATcaatatcgcaatatcg    >  2:164069/1‑140 (MQ=255)                                                                                                                                      aGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCaatatcgcaatatcgc   <  2:252314/140‑1 (MQ=255)                                                                                                                                      aGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCaatatcgcaatatcgc   <  2:1422947/140‑1 (MQ=255)                                                                                                                                      aGATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCaatatcgcaatatcgc   <  1:474804/140‑1 (MQ=255)                                                                                                                                       gATGCGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGcc  <  1:857558/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TCAGTTATTTACGTTATGCCGCGCAAGGATATTTCGCCTCGCCACTCCTGCACTTTGTTCATGCTCTGAGTTGCCCGAAACGTACCGCTGTTGCGATTGATTCGCTGCTGGCGCTGGGGCATACGTCAGGGGCAGATACGCTGCTGGGGTTCTGGCTTGGCCAACAATTATTACAAGGAAAACCATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTGACGGCAGAAAATCAATATCGCAATATCGCC  >  NC_000913/550255‑550528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑