Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,377	A→G	V30A (GTT→GCT)	fldA ←	flavodoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,377	0	A	G	100.0%	223.5 / NA	59	V30A (GTT→GCT)	fldA	flavodoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/21);  total (38/21)

CTCTTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAACGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATT  >  NC_000913/711241‑711511                                                                                                                                         |                                                                                                                                       ctctTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGt                                                                                                                                     <  2:352151/140‑1 (MQ=255)          agTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCaa                                                                                                                            >  2:266451/1‑140 (MQ=255)           gTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAg                                                                                                                           <  2:361332/140‑1 (MQ=255)              gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  1:313892/1‑140 (MQ=255)              gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  1:259971/1‑140 (MQ=255)              gggAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTg                                                                                                                        >  2:1505984/1‑140 (MQ=255)                    aagTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTg                                                                                                                  <  2:686683/140‑1 (MQ=255)                      gTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGaa                                                                                                                >  2:276587/1‑140 (MQ=255)                                  acacTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGa                                                                                                    <  2:1247749/140‑1 (MQ=255)                                     cTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGatat                                                                                                 >  1:1534217/1‑140 (MQ=255)                                         gcTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTc                                                                                             <  2:83345/140‑1 (MQ=255)                                          cTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCg                                                                                            >  2:1410190/1‑140 (MQ=255)                                          cTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCg                                                                                            >  1:1376436/1‑140 (MQ=255)                                             cGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTa                                                                                         >  1:661422/1‑140 (MQ=255)                                             cGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTCCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTa                                                                                         >  1:1416663/1‑140 (MQ=255)                                                       ccAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGgtgt                                                                               >  1:1228563/1‑140 (MQ=255)                                                          ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTTTTACCGGTGTCGc                                                                            <  2:1537211/140‑1 (MQ=255)                                                          ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGc                                                                            >  2:73114/1‑140 (MQ=255)                                                          ggTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGc                                                                            >  1:259374/1‑140 (MQ=255)                                                              gggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGcc                                                                        >  2:136206/1‑140 (MQ=255)                                                               ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  1:1459293/1‑140 (MQ=255)                                                               ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  2:606421/1‑140 (MQ=255)                                                               ggATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCg                                                                       >  1:105181/1‑140 (MQ=255)                                                                    cccAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGaaaaa                                                                  >  1:508050/1‑140 (MQ=255)                                                                    cccAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGaaaaa                                                                  >  1:66787/1‑140 (MQ=255)                                                                                aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  2:900348/140‑1 (MQ=255)                                                                                aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  1:450210/140‑1 (MQ=255)                                                                                aTGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGAt                                                      <  2:65227/140‑1 (MQ=255)                                                                                   tCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGAATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGc                                                   >  1:868756/1‑140 (MQ=255)                                                                                    cATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGcc                                                  <  1:136206/140‑1 (MQ=255)                                                                                      tAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCAt                                                >  2:1403097/1‑140 (MQ=255)                                                                                      tAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTAGCTGCCGAAAAAGATGCCAGTGATAGCCAt                                                <  2:1584808/140‑1 (MQ=255)                                                                                       aaGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATg                                               <  1:266451/140‑1 (MQ=255)                                                                                        aGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGa                                              <  2:592206/140‑1 (MQ=255)                                                                                         gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             <  2:476115/140‑1 (MQ=255)                                                                                         gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             >  2:1238878/1‑140 (MQ=255)                                                                                         gCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAg                                             <  1:276587/140‑1 (MQ=255)                                                                                          cTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGt                                            <  1:183204/140‑1 (MQ=255)                                                                                            tCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGa                                          >  1:1026226/1‑140 (MQ=255)                                                                                                    ttcttTGCTGCTTTTTGCAATGTCATGGACATCGACAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACct                                  >  1:666848/1‑140 (MQ=255)                                                                                                         tgctgcTTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGa                             <  1:1403097/140‑1 (MQ=255)                                                                                                           ctgctTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGaaa                           >  1:1419803/1‑140 (MQ=255)                                                                                                              ctTTTTGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACtt                        >  1:1373938/1‑140 (MQ=255)                                                                                                                ttttGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTAt                      >  1:937971/1‑140 (MQ=255)                                                                                                                ttttGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTAt                      <  2:920903/140‑1 (MQ=255)                                                                                                                   tGCAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGa                   >  2:191128/1‑140 (MQ=255)                                                                                                                     cAATGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGaaa                 <  2:105181/140‑1 (MQ=255)                                                                                                                       aTGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATg               <  2:259374/140‑1 (MQ=255)                                                                                                                       aTGTCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATg               <  2:259971/140‑1 (MQ=255)                                                                                                                          tCATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATgggg            >  1:526769/1‑140 (MQ=255)                                                                                                                           cATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATggggg           >  1:547359/1‑140 (MQ=255)                                                                                                                           cATGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATggggg           >  2:523939/1‑140 (MQ=255)                                                                                                                             tGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATgggggcg         >  2:24935/1‑138 (MQ=255)                                                                                                                             tGGACATCGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTg         >  2:1305846/1‑140 (MQ=255)                                                                                                                                    cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:933877/1‑140 (MQ=255)                                                                                                                                    cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:348545/1‑140 (MQ=255)                                                                                                                                    cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  2:1317027/1‑140 (MQ=255)                                                                                                                                    cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  1:1263346/1‑140 (MQ=255)                                                                                                                                    cGGCAGCGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAtt  >  1:103907/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CTCTTCGAGAGTCGGGAAGAAGTCATCCCAGTCACACTGCGCTTCGCCGTAATACCAGGTTGGGATGCCCAGCAGCAGAATGTCATAAGCTTCCAGATCTTCTTTGCTGCTTTTTGCAATGTCATGGACATCGGCAACGTCTTTACCAAGCTGTTTTTGAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATT  >  NC_000913/711241‑711511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑