Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,028,007	C→T	A21V (GCA→GTA)	yccU →	putative CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,028,007	0	C	T	100.0%	224.8 / NA	69	A21V (GCA→GTA)	yccU	putative CoA‑binding protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/30);  total (39/30)

TCATTCATTCGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGA  >  NC_000913/1027871‑1028142                                                                                                                                         |                                                                                                                                        tcattcatTCGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAg                                                                                                                                      <  1:296678/140‑1 (MQ=255)   attcattcGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCg                                                                                                                                    >  2:178719/1‑140 (MQ=255)   attcattcGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCg                                                                                                                                    <  2:592302/140‑1 (MQ=255)    ttcattcGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGa                                                                                                                                   <  1:167482/140‑1 (MQ=255)             aTTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGa                                                                                                                          >  2:236727/1‑140 (MQ=255)             aTTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGa                                                                                                                          >  1:1052948/1‑140 (MQ=255)               tAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATc                                                                                                                        <  2:1520593/140‑1 (MQ=255)               tAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATc                                                                                                                        <  1:214992/140‑1 (MQ=255)               tAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATc                                                                                                                        >  2:1249611/1‑140 (MQ=255)                   tGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTcc                                                                                                                    >  1:590512/1‑140 (MQ=255)                   tGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTcc                                                                                                                    >  2:813759/1‑140 (MQ=255)                   tGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTcc                                                                                                                    >  2:1300200/1‑140 (MQ=255)                     cATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCa                                                                                                                  >  2:148416/1‑140 (MQ=255)                               aTGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGt                                                                                                        >  1:1560363/1‑140 (MQ=255)                               aTGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGt                                                                                                        >  2:363131/1‑140 (MQ=255)                                             gTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTAc                                                                                          <  1:1293271/140‑1 (MQ=255)                                             gTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTAc                                                                                          <  2:1494532/140‑1 (MQ=255)                                             gTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTAc                                                                                          <  1:382336/140‑1 (MQ=255)                                              tACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt                                                                                         <  1:877476/140‑1 (MQ=255)                                              tACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt                                                                                         <  2:1283145/140‑1 (MQ=255)                                              tACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt                                                                                         <  2:347715/140‑1 (MQ=255)                                              tACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACt                                                                                         <  1:236727/140‑1 (MQ=255)                                                 acTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGa                                                                                      <  2:462531/140‑1 (MQ=255)                                                   tttGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGAcc                                                                                    <  2:735566/140‑1 (MQ=255)                                                     tGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAg                                                                                  >  2:1414823/1‑140 (MQ=255)                                                     tGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAg                                                                                  >  2:150550/1‑140 (MQ=255)                                                     tGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGGGGGGGTAAGCGACAAACCCGACCGTCCCAGCTATCGCGCGATGAAATATCTACTCGACCAg                                                                                  >  2:1370827/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  2:158432/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  2:1468724/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  2:980874/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  1:694100/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  1:499671/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  2:633622/1‑140 (MQ=255)                                                               tCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACg                                                                        >  2:905712/1‑140 (MQ=255)                                                                cTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGt                                                                       <  1:528438/140‑1 (MQ=255)                                                                         ataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGt                                                              >  1:113825/1‑140 (MQ=255)                                                                         ataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGt                                                              >  1:706129/1‑140 (MQ=255)                                                                         ataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGt                                                              >  2:1448567/1‑140 (MQ=255)                                                                         ataatGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGAATCAAGTCATTCCGGt                                                              >  1:511112/1‑140 (MQ=255)                                                                             tGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCg                                                          >  1:591566/1‑140 (MQ=255)                                                                              gaaagaaaCCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGc                                                         >  2:126567/1‑140 (MQ=255)                                                                                         atatTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGcc                                              >  1:534340/1‑140 (MQ=255)                                                                                             tGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTGATTCCGGTTTCGCCAAAAGTTGCCGGCa                                          >  2:976703/1‑140 (MQ=255)                                                                                                aGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCaaaa                                       <  1:1438230/140‑1 (MQ=255)                                                                                                    aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACgct                                   >  2:406910/1‑140 (MQ=255)                                                                                                    aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACgct                                   >  2:920099/1‑140 (MQ=255)                                                                                                    aTTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACgct                                   >  1:45712/1‑140 (MQ=255)                                                                                                     ttttAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACgctg                                  <  2:1078747/140‑1 (MQ=255)                                                                                                      tttAACGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACgctgc                                 <  1:993333/140‑1 (MQ=255)                                                                                                           cGTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGc                            <  1:1316579/140‑1 (MQ=255)                                                                                                            gTCCACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGcc                           >  1:116018/1‑140 (MQ=255)                                                                                                              ccACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCTCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAg                         <  1:178719/140‑1 (MQ=255)                                                                                                               cACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                        <  1:980874/140‑1 (MQ=255)                                                                                                               cACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                        <  1:1300200/140‑1 (MQ=255)                                                                                                               cACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                        <  1:430641/140‑1 (MQ=255)                                                                                                               cACTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                        <  1:270934/140‑1 (MQ=255)                                                                                                                 cTCACACCATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGACATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAg                      <  1:1468724/140‑1 (MQ=255)                                                                                                                        cATTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATg               >  1:996940/1‑140 (MQ=255)                                                                                                                         aTTGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATgg              >  2:492012/1‑140 (MQ=255)                                                                                                                           tGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCa            <  2:707220/140‑1 (MQ=255)                                                                                                                           tGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCa            <  1:363131/140‑1 (MQ=255)                                                                                                                           tGCGCTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCa            >  2:13001/1‑140 (MQ=255)                                                                                                                            gcgcTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCAc           >  2:7644/1‑140 (MQ=255)                                                                                                                            gcgcTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCAc           >  1:378595/1‑140 (MQ=255)                                                                                                                            gcgcTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCAc           <  1:92598/140‑1 (MQ=255)                                                                                                                            gcgcTGGCGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCAc           >  1:1409130/1‑140 (MQ=255)                                                                                                                             cgcTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACg          <  2:499671/140‑1 (MQ=255)                                                                                                                              gcTGGTGGGGGTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGc         >  1:1118245/1‑140 (MQ=255)                                                                                                                                  gtggGGGTAAGCGACAAACCCGATAGTCCCAGCTATCGCGTGATGAAATATCTTCTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGCAGGGGCATGGCACGCTGGc     >  2:907935/1‑140 (MQ=255)                                                                                                                                     ggggTAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGa  <  2:590512/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCATTCATTCGTATTAGCTGCATGGTTGGCATGTCGGCTTTGTCGTACACTTTGTTAAACAGTCTGGAGGAAATAATGAAAGAAACCGATATTGCAGGCATTTTAACGTCCACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGA  >  NC_000913/1027871‑1028142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑