Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,099	T→C	T116A (ACC→GCC)	potB ←	spermidine preferential ABC transporter membrane subunit PotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,099	0	T	C	100.0%	298.0 / NA	79	T116A (ACC→GCC)	potB	spermidine preferential ABC transporter membrane subunit PotB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/32);  total (47/32)

CAGCGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGTGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCACC  >  NC_000913/1183968‑1184235                                                                                                                                    |                                                                                                                                         cAGCGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGa                                                                                                                                  >  2:623543/1‑140 (MQ=255)    cGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTTGATGAGATAGCCTTTGGCGCAGAGGaaaa                                                                                                                               >  1:773390/1‑140 (MQ=255)    cGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGaaaa                                                                                                                               >  1:210353/1‑140 (MQ=255)    cGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGaaaa                                                                                                                               >  1:534725/1‑140 (MQ=255)    cGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGaaaa                                                                                                                               >  2:25752/1‑140 (MQ=255)    cGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGaaaa                                                                                                                               >  2:624324/1‑140 (MQ=255)      gCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAAtt                                                                                                                             >  2:326792/1‑140 (MQ=255)      gCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAAtt                                                                                                                             >  2:79894/1‑140 (MQ=255)      gCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAAtt                                                                                                                             >  2:750241/1‑140 (MQ=255)         tCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTc                                                                                                                          >  2:559947/1‑140 (MQ=255)              aTAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGccc                                                                                                                     <  1:440699/140‑1 (MQ=255)               tAAACGGCAGCAGAATGTAAACCATACCGATAATCACTGCACTGGGCGTGAACATGATACGGATAGGTGTGTCGATAACGCCCAGCCAGCACAAAAACTCGTTTAGACAGCCTTTGGTACTGAAGAAAATTTACAGCCCg                                                                                                                    >  2:1125901/1‑140 (MQ=255)                   cGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGa                                                                                                                <  2:875105/140‑1 (MQ=255)                    ggCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGAt                                                                                                               <  1:175712/140‑1 (MQ=255)                            aaTGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTa                                                                                                       <  2:172810/140‑1 (MQ=255)                             aTGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTaa                                                                                                      <  1:154122/140‑1 (MQ=255)                              tGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAAt                                                                                                     >  1:767815/1‑140 (MQ=255)                                  atccAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAATCTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCCTAGATGCGAATTAATGAGt                                                                                                 <  2:1345529/138‑1 (MQ=255)                                  aaCCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGt                                                                                                 <  2:965455/140‑1 (MQ=255)                                       gACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTc                                                                                            <  2:433352/140‑1 (MQ=255)                                       gACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTc                                                                                            <  1:225545/140‑1 (MQ=255)                                        aCCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTcc                                                                                           >  2:590025/1‑140 (MQ=255)                                        aCCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTcc                                                                                           >  2:1578090/1‑140 (MQ=255)                                        aCCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTcc                                                                                           >  1:655942/1‑140 (MQ=255)                                        aCCGATAATCACCGCACTGGGCGTGAACATGATACGGAATGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTGAGCCCGTAGATACGAATTAATGAGTTGGTcc                                                                                           >  1:1517027/1‑140 (MQ=255)                                         ccGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCa                                                                                          >  1:1039735/1‑140 (MQ=255)                                           gATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGa                                                                                        <  1:359799/140‑1 (MQ=255)                                                tCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGa                                                                                   <  1:559947/140‑1 (MQ=255)                                                    cGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACaa                                                                               >  2:728856/1‑140 (MQ=255)                                                      cACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATc                                                                             <  1:896289/140‑1 (MQ=255)                                                      cACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATc                                                                             <  2:458385/140‑1 (MQ=255)                                                         tGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATcagc                                                                          >  1:764047/1‑140 (MQ=255)                                                         tGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGCAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATcagc                                                                          >  2:1148442/1‑140 (MQ=255)                                                          gggCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCCTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATcagca                                                                         >  2:1574854/1‑140 (MQ=255)                                                           ggCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATcagcag                                                                        >  1:746352/1‑140 (MQ=255)                                                           ggCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATcagcag                                                                        >  1:133601/1‑140 (MQ=255)                                                            gCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGa                                                                       <  1:326792/140‑1 (MQ=255)                                                             cGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGaa                                                                      >  2:1570268/1‑140 (MQ=255)                                                             cGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGaa                                                                      >  2:528539/1‑140 (MQ=255)                                                              gTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGaaa                                                                     <  1:702991/140‑1 (MQ=255)                                                                 aaCATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcag                                                                  >  2:1471017/1‑140 (MQ=255)                                                                     tGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagc                                                              >  1:186477/1‑140 (MQ=255)                                                                       aTACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            <  1:721971/140‑1 (MQ=255)                                                                       aTACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            >  1:1333810/1‑140 (MQ=255)                                                                       aTACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            >  1:1557638/1‑140 (MQ=255)                                                                       aTACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            <  2:655942/140‑1 (MQ=255)                                                                         aCGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCg                                                          <  1:79894/140‑1 (MQ=255)                                                                         aCGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCg                                                          <  1:25752/140‑1 (MQ=255)                                                                           ggATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAACATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGa                                                        >  1:1423987/1‑140 (MQ=255)                                                                           ggATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGa                                                        <  1:989132/140‑1 (MQ=255)                                                                            gATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGAc                                                       <  2:985032/140‑1 (MQ=255)                                                                             aTTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACg                                                      >  1:742940/1‑140 (MQ=255)                                                                             aTTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACg                                                      >  1:1004565/1‑140 (MQ=255)                                                                             aTTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACg                                                      >  1:1082429/1‑140 (MQ=255)                                                                                 gtgtgtCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCAcc                                                  >  2:1580608/1‑140 (MQ=255)                                                                                      tCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTtgtg                                             >  2:949220/1‑140 (MQ=255)                                                                                      tCGATAACGCCCAGCCAGAGC‑AAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            <  1:422863/124‑1 (MQ=255)                                                                                      tCGATAACGCCCAGCCAGAGC‑AAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAAcagcagcag                                                            >  2:422863/1‑124 (MQ=255)                                                                                       cGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTtgtgt                                            >  2:174874/1‑140 (MQ=255)                                                                                        gATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTtgtgtg                                           <  2:1492546/140‑1 (MQ=255)                                                                                        gATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTtgtgtg                                           <  1:907835/140‑1 (MQ=255)                                                                                         aTAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTgg                                          >  1:152359/1‑140 (MQ=255)                                                                                          tAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGc                                         <  1:250905/140‑1 (MQ=255)                                                                                              gCCCAACCAGAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACt                                     >  2:173066/1‑140 (MQ=255)                                                                                                ccagccagAGCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTc                                   >  2:238529/1‑140 (MQ=255)                                                                                                        agCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGaaa                           >  1:907529/1‑140 (MQ=255)                                                                                                        agCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGaaa                           >  1:347434/1‑140 (MQ=255)                                                                                                        agCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGaaa                           <  1:1148442/140‑1 (MQ=255)                                                                                                        agCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGaaa                           >  1:1108574/1‑140 (MQ=255)                                                                                                        agCAAAAACTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGaaa                           >  1:1038388/1‑140 (MQ=255)                                                                                                            aaaaCTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAg                       >  2:1045356/1‑140 (MQ=255)                                                                                                                cTCGTTGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCaa                   <  1:524216/140‑1 (MQ=255)                                                                                                                    ttGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACgg               >  2:1180512/1‑140 (MQ=255)                                                                                                                     tGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACggg              <  2:1004565/140‑1 (MQ=255)                                                                                                                     tGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACggg              <  1:590025/140‑1 (MQ=255)                                                                                                                     tGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACggg              <  2:767815/140‑1 (MQ=255)                                                                                                                     tGAGATAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACggg              >  1:1045033/1‑140 (MQ=255)                                                                                                                          tAGCCTTTGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGcc         <  1:1164031/140‑1 (MQ=255)                                                                                                                               tttGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCa    <  2:186477/140‑1 (MQ=255)                                                                                                                               tttGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCa    <  1:1471017/140‑1 (MQ=255)                                                                                                                                 tGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCAcc  >  2:174858/1‑140 (MQ=255)                                                                                                                                 tGGCGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCAcc  >  2:873849/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         CAGCGGCATCACCATAAACGGCAGCAGAATGTAAACCAGACCGATAATCACCGCACTGGGCGTGAACATGATACGGATTGGTGTGTCGATAACGCCCAGCCAGAGCAAAAACTCGTTGAGATAGCCTTTGGTGCTGAGGAAAATTTTCAGCCCGTAGATACGAATTAATGAGTTGGTCCAGAACGGAACAATCAGCAGAAACAGCAGCAGCGGACGCACCTTGTGTGGCAACTTCGCCAGAAACCAGGCAAACGGGTAGCCCAGCACC  >  NC_000913/1183968‑1184235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑