Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	43,832	A→G	E215E (GAA→GAG)	fixB →	putative electron transfer flavoprotein FixB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	43,832	0	A	G	100.0%	262.4 / NA	70	E215E (GAA→GAG)	fixB	putative electron transfer flavoprotein FixB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/45);  total (25/45)

GGCAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAACAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCGGTGGG  >  NC_000913/43696‑43966                                                                                                                                         |                                                                                                                                       ggCAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAg                                                                                                                                     <  1:789669/140‑1 (MQ=255)   cAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCt                                                                                                                                   >  2:993248/1‑140 (MQ=255)   cAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCt                                                                                                                                   >  1:1201557/1‑140 (MQ=255)   cAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCt                                                                                                                                   >  2:689888/1‑140 (MQ=255)     ggCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCttt                                                                                                                                 >  1:221546/1‑140 (MQ=255)       cTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGc                                                                                                                               <  1:450877/140‑1 (MQ=255)       cTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGc                                                                                                                               <  1:179684/140‑1 (MQ=255)          cGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAg                                                                                                                            <  2:114915/140‑1 (MQ=255)            gCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGc                                                                                                                          >  1:348408/1‑140 (MQ=255)            gCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGc                                                                                                                          >  1:299617/1‑140 (MQ=255)              tgtgGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGa                                                                                                                        <  1:1125033/140‑1 (MQ=255)               gtgGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGAt                                                                                                                       <  1:550379/140‑1 (MQ=255)               gtgGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGAt                                                                                                                       <  1:1403801/140‑1 (MQ=255)                tgGCGATTACCCGCACGGCAACCCAGGTGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATa                                                                                                                      >  2:536393/1‑140 (MQ=255)                tgGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTTAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGGTa                                                                                                                      >  2:179650/1‑140 (MQ=255)                tgGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATa                                                                                                                      >  1:1313995/1‑140 (MQ=255)                 ggCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGAGATAg                                                                                                                     >  1:867741/1‑140 (MQ=255)                    gATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTg                                                                                                                  <  1:950441/140‑1 (MQ=255)                    gATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTg                                                                                                                  <  2:312586/140‑1 (MQ=255)                    gATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTg                                                                                                                  <  2:89776/140‑1 (MQ=255)                    gATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTg                                                                                                                  <  1:1131360/140‑1 (MQ=255)                      ttACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCg                                                                                                                <  2:1072303/140‑1 (MQ=255)                       tACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCgg                                                                                                               >  1:582103/1‑140 (MQ=255)                         cccGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAg                                                                                                             <  1:190542/140‑1 (MQ=255)                              aCGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGc                                                                                                        <  1:403002/140‑1 (MQ=255)                              aCGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGc                                                                                                        <  1:395143/140‑1 (MQ=255)                              aCGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGc                                                                                                        <  2:1046266/140‑1 (MQ=255)                              aCGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGc                                                                                                        <  1:204240/140‑1 (MQ=255)                                   aaCCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTgat                                                                                                   >  1:1508392/1‑138 (MQ=255)                                         ggCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTc                                                                                             <  2:997610/140‑1 (MQ=255)                                          gcgcgcCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGGATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTcc                                                                                            >  1:1579701/1‑140 (MQ=255)                                               ccAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTgg                                                                                       >  2:460487/1‑140 (MQ=255)                                                    gCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGaa                                                                                  >  2:648281/1‑140 (MQ=255)                                                             tCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGAGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGaaaaa                                                                         >  2:769628/1‑140 (MQ=255)                                                             tCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGCATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGaaaaa                                                                         >  1:206315/1‑140 (MQ=255)                                                                 tctcGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAatgga                                                                     <  2:188648/140‑1 (MQ=255)                                                                    cGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAatggatgg                                                                  <  2:432722/140‑1 (MQ=255)                                                                     gACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAAtggatgga                                                                 <  2:1175799/140‑1 (MQ=255)                                                                      aCAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAg                                                                <  2:466896/140‑1 (MQ=255)                                                                       cAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGc                                                               >  2:1015583/1‑140 (MQ=255)                                                                          aGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACg                                                            <  2:582103/140‑1 (MQ=255)                                                                            cccGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGaa                                                          <  1:805202/140‑1 (MQ=255)                                                                              cGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACg                                                        <  1:1463086/140‑1 (MQ=255)                                                                              cGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACg                                                        <  2:962367/140‑1 (MQ=255)                                                                                tCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCt                                                      <  2:1508392/140‑1 (MQ=255)                                                                                   ggtggtCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATg                                                   <  1:993248/140‑1 (MQ=255)                                                                                   ggtggtCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATg                                                   <  1:1211324/140‑1 (MQ=255)                                                                                       gtCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCgg                                               <  1:925595/140‑1 (MQ=255)                                                                                       gtCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCgg                                               <  2:221546/140‑1 (MQ=255)                                                                                        tCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGt                                              <  2:1221788/140‑1 (MQ=255)                                                                                        tCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGt                                              <  1:1150411/140‑1 (MQ=255)                                                                                         cAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTa                                             <  1:689888/140‑1 (MQ=255)                                                                                          aGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTAt                                            >  1:1515537/1‑140 (MQ=255)                                                                                            cGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTAtct                                          >  2:297057/1‑140 (MQ=255)                                                                                             gtcggtcgCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTAtctc                                         <  2:1471353/140‑1 (MQ=255)                                                                                                 gtcgCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAAc                                     >  2:205335/1‑140 (MQ=255)                                                                                                    gcgGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTg                                  <  2:6736/140‑1 (MQ=255)                                                                                                           tGGCAGCAAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGa                           >  1:231730/1‑140 (MQ=255)                                                                                                              cagcaAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAAc                        <  2:206315/140‑1 (MQ=255)                                                                                                              cagcaAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAAc                        <  1:1401955/140‑1 (MQ=255)                                                                                                              cagcaAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGAGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAAc                        <  1:769628/140‑1 (MQ=255)                                                                                                               agcaAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAAcc                       <  1:801844/140‑1 (MQ=255)                                                                                                                gcaAAGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCt                      >  1:814838/1‑140 (MQ=255)                                                                                                                  aaaGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGa                    >  2:962439/1‑140 (MQ=255)                                                                                                                   aaGAGAACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGaa                   <  1:1457262/140‑1 (MQ=255)                                                                                                                     gagaACATTGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACt                 <  1:492427/140‑1 (MQ=255)                                                                                                                            ttGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTg          <  2:1547029/140‑1 (MQ=255)                                                                                                                             tGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTgg         >  2:564819/1‑140 (MQ=255)                                                                                                                             tGCGCTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTgg         >  2:1222897/1‑140 (MQ=255)                                                                                                                               cgcTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCg       <  2:765490/140‑1 (MQ=255)                                                                                                                                gcTGGCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCgg      >  2:1037728/1‑140 (MQ=255)                                                                                                                                    gCAGAGCAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCGGTggg  <  1:750900/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGCAGGCTCCGGCTGTGGCGATTACCCGCACGGCAACCCAGGCGCGCCAGAGCAACAGCGTCGATCTCGACAAAGCCCGTCTGGTGGTCAGCGTCGGTCGCGGTATTGGCAGCAAAGAGAACATTGCGCTGGCAGAACAGCTTTGCAAGGCGATAGGTGCGGAGTTGGCCTGTTCTCGTCCGGTGGCGGAAAACGAAAAATGGATGGAGCACGAACGCTATGTCGGTATCTCCAACCTGATGCTGAAACCTGAACTGTACCTGGCGGTGGG  >  NC_000913/43696‑43966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑