Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,262,821	A→G	G19G (GGT→GGC)	ispE ←	4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,262,821	0	A	G	100.0%	254.3 / NA	70	G19G (GGT→GGC)	ispE	4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/37);  total (33/37)

ATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGACCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATTGGCTGGCATC  >  NC_000913/1262686‑1262952                                                                                                                                        |                                                                                                                                    aTCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCgg                                                                                                                                 <  1:1185464/140‑1 (MQ=255)  tCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGt                                                                                                                                >  2:1554031/1‑140 (MQ=255)  tCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGt                                                                                                                                <  2:107559/140‑1 (MQ=255)    ttCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTaa                                                                                                                              <  2:440816/140‑1 (MQ=255)    ttCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTaa                                                                                                                              <  1:323016/140‑1 (MQ=255)    ttCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTaa                                                                                                                              >  2:1212448/1‑140 (MQ=255)    ttCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTaa                                                                                                                              <  2:373473/140‑1 (MQ=255)        tGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGta                                                                                                                          >  1:559681/1‑140 (MQ=255)         gTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAACCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGtat                                                                                                                         >  2:652287/1‑140 (MQ=255)           tCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATaa                                                                                                                       >  1:409638/1‑140 (MQ=255)            ccACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCCGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATaaa                                                                                                                      <  1:1109827/140‑1 (MQ=255)              aCGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATaaaaa                                                                                                                    <  1:506448/140‑1 (MQ=255)                gCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACa                                                                                                                  >  1:661087/1‑140 (MQ=255)                  cTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGa                                                                                                                <  1:759961/140‑1 (MQ=255)                     cAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTa                                                                                                             >  2:187663/1‑140 (MQ=255)                     cAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTa                                                                                                             >  1:1217825/1‑140 (MQ=255)                       aCGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAg                                                                                                           <  2:1430737/140‑1 (MQ=255)                             gTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGc                                                                                                     <  1:757750/140‑1 (MQ=255)                               tAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCg                                                                                                   <  1:788332/140‑1 (MQ=255)                                 aCAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGga                                                                                                 <  1:1000829/140‑1 (MQ=255)                                   aGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGgaga                                                                                               <  2:1074819/140‑1 (MQ=255)                                     aCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAgg                                                                                             >  2:444777/1‑140 (MQ=255)                                     aCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAgg                                                                                             >  2:894240/1‑140 (MQ=255)                                     aCGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAgg                                                                                             >  2:1542697/1‑140 (MQ=255)                                         atatCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTATCCATCCGCACGCTGGTCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCa                                                                                         <  2:696215/140‑1 (MQ=255)                                         atatCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCa                                                                                         >  2:1029538/1‑140 (MQ=255)                                         atatCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCa                                                                                         >  2:1507318/1‑140 (MQ=255)                                             ccccATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACtgt                                                                                     <  2:524979/140‑1 (MQ=255)                                                  tCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCg                                                                                <  1:756204/140‑1 (MQ=255)                                                  tCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCg                                                                                <  1:1262237/140‑1 (MQ=255)                                                   cGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGc                                                                               >  1:289088/1‑140 (MQ=255)                                                   cGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGc                                                                               >  1:1550585/1‑140 (MQ=255)                                                   cGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGc                                                                               >  1:927896/1‑140 (MQ=255)                                                    gTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATGAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCa                                                                              <  1:782354/140‑1 (MQ=255)                                                    gTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCa                                                                              <  1:1449122/140‑1 (MQ=255)                                                       aCGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCatta                                                                           <  2:1295237/140‑1 (MQ=255)                                                       aCGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCatta                                                                           <  1:1554031/140‑1 (MQ=255)                                                       aCGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCatta                                                                           <  2:886333/140‑1 (MQ=255)                                                          aaGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTAttt                                                                        <  2:409638/140‑1 (MQ=255)                                                                 aTGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATc                                                                 <  1:336902/140‑1 (MQ=255)                                                                       aTGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTa                                                           >  2:837275/1‑140 (MQ=255)                                                                                     aTCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACtt                                             <  2:95310/140‑1 (MQ=255)                                                                                     aTCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACtt                                             >  1:599275/1‑140 (MQ=255)                                                                                      tCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTg                                            <  1:894240/140‑1 (MQ=255)                                                                                       cAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGa                                           >  1:847378/1‑140 (MQ=255)                                                                                        aaGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGAt                                          <  2:868216/140‑1 (MQ=255)                                                                                           aaaCTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCg                                       <  2:1217825/140‑1 (MQ=255)                                                                                           aaaCTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCg                                       >  1:273449/1‑140 (MQ=255)                                                                                             aCTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGtt                                     <  1:413334/140‑1 (MQ=255)                                                                                                gAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGac                                  <  1:1212448/140‑1 (MQ=255)                                                                                                gAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGac                                  <  2:559681/140‑1 (MQ=255)                                                                                                gAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGac                                  <  1:1507318/140‑1 (MQ=255)                                                                                                  aaCAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGacca                                >  1:1247437/1‑140 (MQ=255)                                                                                                      gCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGt                            >  1:772523/1‑140 (MQ=255)                                                                                                         tttGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCgg                         >  2:1542031/1‑140 (MQ=255)                                                                                                          ttGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGt                        <  1:444777/140‑1 (MQ=255)                                                                                                          ttGCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGt                        <  2:1327235/140‑1 (MQ=255)                                                                                                            gCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGa                      >  1:196796/1‑140 (MQ=255)                                                                                                            gCAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGa                      >  2:80785/1‑140 (MQ=255)                                                                                                             cAGCGTGTGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAg                     <  2:661087/140‑1 (MQ=255)                                                                                                                 gtgtGGTAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTcc                 >  2:1448068/1‑140 (MQ=255)                                                                                                                       tAACCATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtg           <  1:1031440/140‑1 (MQ=255)                                                                                                                           cATCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtggctg       >  2:563973/1‑140 (MQ=255)                                                                                                                            aTCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtggctgg      >  2:76023/1‑140 (MQ=255)                                                                                                                            aTCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtggctgg      >  2:14386/1‑140 (MQ=255)                                                                                                                             tCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtggctggc     >  2:409891/1‑140 (MQ=255)                                                                                                                             tCCGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATtggctggc     >  1:881518/1‑140 (MQ=255)                                                                                                                              ccGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATTGGCTGGCa    >  1:1556563/1‑140 (MQ=255)                                                                                                                              ccGCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATTGGCTGGCa    >  1:182363/1‑140 (MQ=255)                                                                                                                                gCACGCTGGCCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATTGGCTGGCATc  <  2:273449/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    ATCTTCATGTTCCACGCCTTCAACGGGCGTTAACAGACGAATATCCCCATCGTCACGAAGCTCAATGCTGATGGTGTCGCCGTAATCAAGAAACTGAAACAGCGTTTGCAGCGTGTGGTAACCATCCGCACGCTGACCGGTAATGTATAAAAACAGATTAAGTTTTGCCGGAGAGGGCCACTGTGTCCGCATTATTTCACTATCCAGTTATCCATTTTTAACTTGATGCGTTGACCACCGTCGGTGAGTTCCATATTGGCTGGCATC  >  NC_000913/1262686‑1262952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑