Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,273,790	C→T	R3C (CGT→TGT)	ychO →	putative invasin YchO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,273,790	0	C	T	100.0%	249.6 / NA	71	R3C (CGT→TGT)	ychO	putative invasin YchO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/39);  total (32/39)

TGCAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCCGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGCCGGATTTAG  >  NC_000913/1273653‑1273922                                                                                                                                          |                                                                                                                                     tGCAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGt                                                                                                                                    >  2:1480391/1‑140 (MQ=255) tGCAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGt                                                                                                                                    >  2:1108243/1‑140 (MQ=255)   cAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGttt                                                                                                                                  <  1:750146/140‑1 (MQ=255)       gTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGtt                                                                                                                              >  1:1510320/1‑140 (MQ=255)       gTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGtt                                                                                                                              >  2:1152168/1‑140 (MQ=255)        tCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTc                                                                                                                             >  2:1324516/1‑140 (MQ=255)        tCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTc                                                                                                                             >  2:327650/1‑140 (MQ=255)         cTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTcc                                                                                                                            <  2:14127/140‑1 (MQ=255)          tGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCt                                                                                                                           >  2:1420511/1‑140 (MQ=255)           gACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTc                                                                                                                          >  2:433687/1‑140 (MQ=255)             ccAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGc                                                                                                                        >  2:1434650/1‑140 (MQ=255)                 tCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTa                                                                                                                    >  2:1409531/1‑140 (MQ=255)                 tCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTa                                                                                                                    >  1:1319826/1‑140 (MQ=255)                       acaTTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGt                                                                                                              <  1:1413368/140‑1 (MQ=255)                       acaTTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGt                                                                                                              <  2:1253742/140‑1 (MQ=255)                       acaTTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGt                                                                                                              <  1:593565/140‑1 (MQ=255)                             ttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTtttatt                                                                                                        >  2:435566/1‑140 (MQ=255)                                 ggATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTAct                                                                                                    <  1:925319/140‑1 (MQ=255)                                  gATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTActc                                                                                                   <  1:1056871/140‑1 (MQ=255)                                   aTTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTActct                                                                                                  >  1:1346876/1‑140 (MQ=255)                                           gAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTa                                                                                          <  1:224561/140‑1 (MQ=255)                                           gAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTa                                                                                          <  1:697444/140‑1 (MQ=255)                                             aaGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGc                                                                                        <  2:35310/140‑1 (MQ=255)                                              aGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCa                                                                                       >  2:330044/1‑140 (MQ=255)                                               gcgcGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAg                                                                                      <  2:411455/140‑1 (MQ=255)                                                cgcgAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAgg                                                                                     <  2:814122/140‑1 (MQ=255)                                                  cgAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCg                                                                                   >  1:1271917/1‑140 (MQ=255)                                                  cgAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTGTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCg                                                                                   >  2:1175390/1‑140 (MQ=255)                                                    agaAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGt                                                                                 <  1:107085/140‑1 (MQ=255)                                                    agaAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGt                                                                                 >  1:1497563/1‑140 (MQ=255)                                                    agaAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGt                                                                                 >  1:1416062/1‑140 (MQ=255)                                                      aaaaTACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTAc                                                                               <  1:1154939/140‑1 (MQ=255)                                                         aTACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGc                                                                            <  2:898304/140‑1 (MQ=255)                                                          tACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCt                                                                           >  2:911819/1‑140 (MQ=255)                                                          tACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCt                                                                           <  1:1152168/140‑1 (MQ=255)                                                          tACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCt                                                                           <  2:866062/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  2:549622/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  1:1104774/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  1:1403288/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  1:1420511/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  2:725191/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  1:227978/140‑1 (MQ=255)                                                                aaaaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGca                                                                     <  1:327650/140‑1 (MQ=255)                                                                  aaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGcaca                                                                   <  1:4775/140‑1 (MQ=255)                                                                    gTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAAt                                                                 <  2:666212/140‑1 (MQ=255)                                                                    gTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAAt                                                                 <  2:1054434/140‑1 (MQ=255)                                                                            tAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTc                                                         >  2:934979/1‑140 (MQ=255)                                                                               tCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAg                                                      <  1:433687/140‑1 (MQ=255)                                                                                        tttAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCg                                             <  1:1433256/140‑1 (MQ=255)                                                                                        tttAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCg                                             >  2:1562979/1‑140 (MQ=255)                                                                                          tAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGc                                           <  2:347020/140‑1 (MQ=255)                                                                                            aGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCaa                                         <  2:1416062/140‑1 (MQ=255)                                                                                             ggCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCaaa                                        >  1:55579/1‑140 (MQ=255)                                                                                             ggCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCaaa                                        <  2:491394/140‑1 (MQ=255)                                                                                                tATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATcc                                     >  1:1105101/1‑140 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   <  2:1346876/140‑1 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   <  2:1319826/140‑1 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   >  1:914729/1‑140 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   >  2:413663/1‑140 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   <  2:1336801/140‑1 (MQ=255)                                                                                                  tGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCt                                   >  2:1422622/1‑140 (MQ=255)                                                                                                    gTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCttt                                 <  1:430429/140‑1 (MQ=255)                                                                                                       tttCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGat                              <  1:934979/140‑1 (MQ=255)                                                                                                        ttCATTATTACCGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGata                             >  1:185228/1‑140 (MQ=255)                                                                                                                  ccGGAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAatga                   <  1:641110/140‑1 (MQ=255)                                                                                                                    ggAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAatgatg                 >  1:448568/1‑140 (MQ=255)                                                                                                                     gAAGTTACCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGTAAAAAGCGGCAAATCCCTTTGATAATAACAATGATgg                >  1:1276301/1‑140 (MQ=255)                                                                                                                           aCCGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGcc          >  1:970906/1‑140 (MQ=255)                                                                                                                             cGACGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGCCgg        >  2:1467290/1‑140 (MQ=255)                                                                                                                                cGTTTTGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGCCGGAtt     <  1:1434650/140‑1 (MQ=255)                                                                                                                                   tttGAGCTGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGCCGGATTTAg  >  2:127459/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     TGCAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTTACCGACGTTTTGAGCCGTTTCGTTCCTCGCATTATCCCGTTTTATTTACTCTTGCTTGTAGCAGGCGGTACAGCTAACGCACAATCTACCTTCGAGCAAAAAGCGGCAAATCCCTTTGATAATAACAATGATGGTCTGCCGGATTTAG  >  NC_000913/1273653‑1273922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑