Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,307	T→C	I110V (ATT→GTT)	sapF ←	putrescine ABC exporter ATP binding protein SapF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,307	0	T	C	100.0%	332.1 / NA	88	I110V (ATT→GTT)	sapF	putrescine ABC exporter ATP binding protein SapF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/49);  total (39/49)

TGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGG  >  NC_000913/1352171‑1352439                                                                                                                                         |                                                                                                                                     tGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTg                                                                                                                                   >  2:1393337/1‑140 (MQ=255)  gTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGc                                                                                                                                  <  1:1235078/140‑1 (MQ=255)   tCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCg                                                                                                                                 <  2:605768/140‑1 (MQ=255)   tCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCg                                                                                                                                 <  2:1086628/140‑1 (MQ=255)       ggTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAGTCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGAt                                                                                                                             >  1:96426/1‑140 (MQ=255)       ggTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGAt                                                                                                                             >  1:374353/1‑140 (MQ=255)          gCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACg                                                                                                                          <  1:1436518/140‑1 (MQ=255)             aaCATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTg                                                                                                                       >  2:393428/1‑140 (MQ=255)             aaCATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTg                                                                                                                       >  1:578463/1‑140 (MQ=255)                atatGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACg                                                                                                                    <  1:1561732/140‑1 (MQ=255)                  atGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACggg                                                                                                                  >  1:1354533/1‑140 (MQ=255)                  atGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGAAAAACCCAGACCTGCCGGGATCCGTTGAgggg                                                                                                                  >  1:736780/1‑140 (MQ=255)                         tAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCaa                                                                                                           <  2:1521691/140‑1 (MQ=255)                         tAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCaa                                                                                                           >  1:780936/1‑140 (MQ=255)                          aGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAAc                                                                                                          >  2:165193/1‑140 (MQ=255)                          aGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAAc                                                                                                          >  2:1002436/1‑140 (MQ=255)                          aGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAAc                                                                                                          <  1:218118/140‑1 (MQ=255)                          aGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCCGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAAc                                                                                                          >  1:1013149/1‑140 (MQ=255)                           gTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACg                                                                                                         <  1:857364/140‑1 (MQ=255)                           gTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACg                                                                                                         <  1:1333346/140‑1 (MQ=255)                            tAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGa                                                                                                        <  1:678031/140‑1 (MQ=255)                            tAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGa                                                                                                        >  1:1334127/1‑140 (MQ=255)                              aCTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAgg                                                                                                      >  2:1139397/1‑140 (MQ=255)                                  aCGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGa                                                                                                  <  2:945950/140‑1 (MQ=255)                                  aCGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGa                                                                                                  >  2:1475370/1‑140 (MQ=255)                                    gTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAg                                                                                                >  1:1548372/1‑140 (MQ=255)                                      gATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGa                                                                                              <  2:1302109/140‑1 (MQ=255)                                      gATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGa                                                                                              >  1:722044/1‑140 (MQ=255)                                        tCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATc                                                                                            <  2:1601869/140‑1 (MQ=255)                                                 agcCCAACCATACGCATCGTTTCAATAGTCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAAt                                                                                   <  2:96426/140‑1 (MQ=255)                                                 agcCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAAt                                                                                   >  1:1428082/1‑140 (MQ=255)                                                  gcCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGGTTCAACGAGGTCGAAGGATCCTGAAAAATc                                                                                  >  1:45594/1‑140 (MQ=255)                                                   cccAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCa                                                                                 <  1:1370953/140‑1 (MQ=255)                                                   cccAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCa                                                                                 >  1:1541864/1‑140 (MQ=255)                                                     cAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATg                                                                               <  2:822491/140‑1 (MQ=255)                                                           tACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATa                                                                         >  2:850371/1‑140 (MQ=255)                                                             gggcgcGTTTCAAAAAACCGGTTGCCACGGCGTTCCGGTTTCAGGTCGGGGATCAGGCGGCGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACg                                                                       <  2:736780/135‑1 (MQ=255)                                                                aTCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTg                                                                    >  1:48182/1‑140 (MQ=255)                                                                aTCGTTTCAATAATCTGTTTGCGACGCTGATCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTg                                                                    >  1:525317/1‑140 (MQ=255)                                                                  cGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                  >  2:329529/1‑140 (MQ=255)                                                                  cGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                  >  2:820225/1‑140 (MQ=255)                                                                  cGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                  >  2:473096/1‑140 (MQ=255)                                                                  cGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                  <  1:1007756/140‑1 (MQ=255)                                                                  cGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGAc                                                                  >  2:1193464/1‑140 (MQ=255)                                                                   gTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACt                                                                 >  1:303537/1‑140 (MQ=255)                                                                    tttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTg                                                                <  1:613100/140‑1 (MQ=255)                                                                     ttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGc                                                               >  2:27436/1‑140 (MQ=255)                                                                     ttCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGc                                                               >  2:153071/1‑140 (MQ=255)                                                                          taatCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCggaag                                                          >  1:832904/1‑140 (MQ=255)                                                                              cTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaat                                                      >  2:368532/1‑140 (MQ=255)                                                                               tGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaata                                                     >  1:1042357/1‑140 (MQ=255)                                                                               tGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaata                                                     >  1:1068330/1‑140 (MQ=255)                                                                               tGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaata                                                     >  1:218851/1‑140 (MQ=255)                                                                                gTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaataa                                                    >  2:1232143/1‑140 (MQ=255)                                                                                 tttGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGaataat                                                   <  1:1393337/140‑1 (MQ=255)                                                                                  ttGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATc                                                  >  1:83151/1‑140 (MQ=255)                                                                                  ttGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATc                                                  >  1:767240/1‑140 (MQ=255)                                                                                        cGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGATGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCaaa                                            <  2:1179378/140‑1 (MQ=255)                                                                                        cGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCaaa                                            <  1:393428/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  2:769591/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  1:850371/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  1:27436/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  2:1548372/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         >  2:540961/1‑140 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  1:165193/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  1:917741/140‑1 (MQ=255)                                                                                           tGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATg                                         <  1:1139397/140‑1 (MQ=255)                                                                                                  gTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGga                                  <  2:1428082/140‑1 (MQ=255)                                                                                                  gTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGga                                  <  2:1525207/140‑1 (MQ=255)                                                                                                  gTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGga                                  <  2:1013149/140‑1 (MQ=255)                                                                                                   ttCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgat                                 <  1:250566/140‑1 (MQ=255)                                                                                                   ttCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgat                                 <  2:780936/140‑1 (MQ=255)                                                                                                      cAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGGTTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgatgat                              <  2:45594/140‑1 (MQ=255)                                                                                                      cAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGgatgat                              <  2:212073/140‑1 (MQ=255)                                                                                                       aGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATc                             >  2:809975/1‑140 (MQ=255)                                                                                                         gTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGt                           <  2:832904/140‑1 (MQ=255)                                                                                                         gTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGt                           <  2:869219/140‑1 (MQ=255)                                                                                                         gTCGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGt                           <  1:820225/140‑1 (MQ=255)                                                                                                           cGGTGTTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGtca                         <  1:1599783/140‑1 (MQ=255)                                                                                                             gtgtTCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGtcaat                       >  1:71750/1‑140 (MQ=255)                                                                                                                 tCAGGCGCAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTcaatcaat                   <  1:128771/140‑1 (MQ=255)                                                                                                                     gcgcAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACt               <  1:1193464/140‑1 (MQ=255)                                                                                                                     gcgcAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACt               <  1:1002436/140‑1 (MQ=255)                                                                                                                     gcgcAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCATCAATCAATAACt               <  1:1475370/140‑1 (MQ=255)                                                                                                                        cAGTGGAAAATCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTcgc            <  1:282908/140‑1 (MQ=255)                                                                                                                              aaaaTCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTgg      <  2:1363786/140‑1 (MQ=255)                                                                                                                               aaaTCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGt     <  2:374353/140‑1 (MQ=255)                                                                                                                               aaaTCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGt     <  1:368532/140‑1 (MQ=255)                                                                                                                                  tCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAgg  <  1:218271/140‑1 (MQ=255)                                                                                                                                  tCCAGAACTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAgg  <  1:329529/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGTCCGGGTGCCAACATATGCGGATAGTAACTGACGTGATCCGGTAGCAGCCCAACCATACGCATCGTTTCAATAATCTGTTTGCGACGCTGTTCCGGTTCCAGGTCGGTGTTCAGGCGCAGTGGAAAATCCAGAATTTGCGAGATACGTTGACGGGGATTCAACGAGGTCGAAGGATCCTGAAAAATCATGCGAATACGCTGACTGCGGAAGGAATAATCGCCAAAATGCAGTGGATGATCGTCAATCAATAACTCGCCGCTGGTAGG  >  NC_000913/1352171‑1352439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑