| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,422,015 | A→G | E11E (GAA→GAG) | ydaV → | Rac prophage; putative ATP‑binding protein YdaV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,422,015 | 0 | A | G | 98.3% | 214.5 / ‑4.4 | 59 | E11E (GAA→GAG) | ydaV | Rac prophage; putative ATP‑binding protein YdaV |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (34/24); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
GTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGAAA > NC_000913/1421880‑1422151 | gTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTa > 2:1241640/1‑140 (MQ=255) ttCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTAt > 1:710658/1‑140 (MQ=255) tCGCGCCGAAAGGTGTTCGCCCTGAGACGTTCCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATc < 1:181014/140‑1 (MQ=255) cgcCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGc > 2:994991/1‑140 (MQ=255) cGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGa < 2:287303/140‑1 (MQ=255) ggTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGc < 2:78566/140‑1 (MQ=255) gtgtTCGACCTGAGACGTTCCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGcc < 1:326986/140‑1 (MQ=255) gtgtTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGcc < 2:87865/140‑1 (MQ=255) tCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGt < 1:5022/140‑1 (MQ=255) tCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGt > 1:651234/1‑140 (MQ=255) cGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTc < 2:1167458/140‑1 (MQ=255) gagaCGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGt > 1:654630/1‑140 (MQ=255) tACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACcgcg < 2:1228271/140‑1 (MQ=255) aCTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCgcgc > 1:136942/1‑140 (MQ=255) ccccGACAGGTTAACCCCGTCTCGGTACCGGATAGTGGGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGGCTGGCCCCGTCACATGTAACCGCGcc < 2:762097/138‑1 (MQ=255) cAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCaa < 1:1112609/140‑1 (MQ=255) ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTGAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa > 1:1386108/1‑140 (MQ=255) ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 2:405724/140‑1 (MQ=255) ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 1:994991/140‑1 (MQ=255) ggTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGa < 1:1095963/140‑1 (MQ=255) gTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGAc > 2:1559750/1‑140 (MQ=255) tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGGCCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 1:892119/1‑140 (MQ=255) tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 2:719825/1‑140 (MQ=255) tctcGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGCAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 1:892466/1‑140 (MQ=255) tctcGTTACCGGATAGTGCGATCCAGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAg > 2:1192059/1‑140 (MQ=255) aCCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGcgcg > 1:1055871/1‑140 (MQ=255) cGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAg > 2:1101937/1‑140 (MQ=255) gATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTg > 1:690723/1‑140 (MQ=255) aTAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTgg > 1:453612/1‑140 (MQ=255) tAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGc < 2:213749/140‑1 (MQ=255) tAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGc < 2:892119/140‑1 (MQ=255) gTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCaa > 1:25417/1‑140 (MQ=255) tGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAAc > 2:741334/1‑140 (MQ=255) tGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAAc > 1:723584/1‑140 (MQ=255) cGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACtt > 1:1327849/1‑140 (MQ=255) cccGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCg < 2:1180019/140‑1 (MQ=255) ccGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGa > 1:1581206/1‑140 (MQ=255) cGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGaa < 2:105906/140‑1 (MQ=255) cAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGc < 2:651234/140‑1 (MQ=255) aGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGTCAGAAACg > 1:1096285/1‑140 (MQ=255) ggggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGtt < 2:1386108/140‑1 (MQ=255) ggggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGtt < 2:25417/140‑1 (MQ=255) ggTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTtgt > 1:838619/1‑140 (MQ=255) tAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTga > 1:820734/1‑140 (MQ=255) aaCTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTgag > 1:1106299/1‑140 (MQ=255) aCCATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGAt > 1:606043/1‑140 (MQ=255) cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa > 2:1412742/1‑140 (MQ=255) cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa < 2:136942/140‑1 (MQ=255) cATGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCa > 2:1030385/1‑140 (MQ=255) tGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAAc > 2:638829/1‑140 (MQ=255) tGAAAAATATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAAc < 2:723584/140‑1 (MQ=255) aaaaaTATTGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCg > 2:1059613/1‑140 (MQ=255) ttGCGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAAt < 2:892466/140‑1 (MQ=255) cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:1454950/1‑140 (MQ=255) cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 2:1575994/1‑140 (MQ=255) cGACAGGCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:257115/1‑140 (MQ=255) cGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGt > 1:312192/1‑140 (MQ=255) ggCGATGTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGtt > 2:62128/1‑140 (MQ=255) gTTCTTGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGTCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGt < 2:1096285/140‑1 (MQ=255) tGAGCGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGaaa < 2:654630/140‑1 (MQ=255) | GTTCGCGCCGAAAGGTGTTCGACCTGAGACGTTACTCCGACAGGTTAACACCGTCTCGTTACCGGATAGTGCGATCCCGCCAGGCTTCAGGGGGTAACTGACCATGAAAAATATTGCGACAGGCGATGTTCTTGAACGTATCCGCAGACTGGCCCCGTCACATGTAACCGCGCCATTCAAGACGGTAGCGGAGTGGCGCGAGTGGCAACTTTCCGAAGGCCAGAAACGTTGTGAGGAGATCAACCGTCAGAATCGTCAGTTGCGGGTGGAAA > NC_000913/1421880‑1422151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |