Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,435,192	A→G	V143A (GTG→GCG)	uspF ←	nucleotide binding filament protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,435,192	0	A	G	100.0%	322.0 / NA	86	V143A (GTG→GCG)	uspF	nucleotide binding filament protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/45);  total (41/45)

ATGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCACAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCG  >  NC_000913/1435055‑1435328                                                                                                                                          |                                                                                                                                         aTGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGcgca                                                                                                                                        >  1:1508835/1‑140 (MQ=255) aTGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGcgca                                                                                                                                        >  1:107481/1‑140 (MQ=255) aTGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGcgca                                                                                                                                        >  1:134536/1‑140 (MQ=255) aTGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGcgca                                                                                                                                        >  1:1283702/1‑140 (MQ=255) aTGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGCACGGAATAGAGGGGAAAATGTCAGCACATTTTCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGGTAGTGTCAGCGcgca                                                                                                                                        >  2:484308/1‑140 (MQ=255)       gAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGc                                                                                                                                  <  2:998200/140‑1 (MQ=255)            cccAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGGACGGATTTGAGGGGAAAAAGTCAGCACATTTGCGAAATGAATCAAAAACCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCCACCAGCGCggg                                                                                                                             >  1:776437/1‑139 (MQ=255)             ccaacATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAACCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAg                                                                                                                            >  1:229895/5‑140 (MQ=255)             ccAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAg                                                                                                                            <  2:312541/140‑1 (MQ=255)                      gAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCg                                                                                                                   <  1:709550/140‑1 (MQ=255)                        aaTCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTg                                                                                                                 <  1:1581776/140‑1 (MQ=255)                         aTCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGCGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGa                                                                                                                <  1:1447843/140‑1 (MQ=255)                         aTCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGa                                                                                                                >  1:1288147/1‑140 (MQ=255)                         aTCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGa                                                                                                                >  2:746682/1‑140 (MQ=255)                           cTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACg                                                                                                              <  2:1539306/140‑1 (MQ=255)                           cTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACg                                                                                                              <  1:1228621/140‑1 (MQ=255)                              atatAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCAc                                                                                                           >  2:1128047/1‑140 (MQ=255)                              atatAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTCACGCAc                                                                                                           >  2:1152695/1‑140 (MQ=255)                                  aaaaaaCTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACa                                                                                                       >  2:386233/1‑140 (MQ=255)                                      aaCTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAACCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTg                                                                                                   >  2:781634/1‑140 (MQ=255)                                       aCTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgc                                                                                                  <  2:1283702/140‑1 (MQ=255)                                       aCTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgc                                                                                                  <  1:232553/140‑1 (MQ=255)                                        cTGTGGCGTGTATGGTTCGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGGCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcg                                                                                                 >  1:882005/1‑140 (MQ=255)                                        cTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcg                                                                                                 >  2:289849/1‑140 (MQ=255)                                        cTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcg                                                                                                 >  1:1040366/1‑140 (MQ=255)                                        cTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAACCCGCAGCAATGTGCGGGCGGTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcg                                                                                                 >  2:1044410/1‑140 (MQ=255)                                        cTGTGGCGTGTATGGGACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcg                                                                                                 >  1:148836/1‑140 (MQ=255)                                           tgGCGTGTATGGTACGGGTTAGAGGGGGAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTgcggcg                                                                                              >  2:818101/1‑140 (MQ=255)                                            ggCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGt                                                                                             <  1:1408687/140‑1 (MQ=255)                                                  tATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAAc                                                                                       >  1:1036850/1‑140 (MQ=255)                                                      gTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAg                                                                                   <  2:1376607/140‑1 (MQ=255)                                                      gTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAg                                                                                   <  2:433788/140‑1 (MQ=255)                                                       tACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGc                                                                                  >  1:316504/1‑140 (MQ=255)                                                       tACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGc                                                                                  >  2:1525676/1‑140 (MQ=255)                                                       tACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGc                                                                                  >  2:1454894/1‑140 (MQ=255)                                                       tACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGc                                                                                  >  2:95588/1‑140 (MQ=255)                                                        aCGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCa                                                                                 <  2:1121309/140‑1 (MQ=255)                                                        aCGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCa                                                                                 <  2:27368/140‑1 (MQ=255)                                                          ggATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGa                                                                               >  1:487475/1‑140 (MQ=255)                                                          ggATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGa                                                                               >  2:575129/1‑140 (MQ=255)                                                          ggATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGa                                                                               >  2:1027568/1‑140 (MQ=255)                                                           gATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGAt                                                                              <  2:941964/140‑1 (MQ=255)                                                            aTTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGTGTTGGAACCGAGCAGATa                                                                             >  1:323437/1‑140 (MQ=255)                                                            aTTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATa                                                                             >  1:1351314/1‑140 (MQ=255)                                                            aTTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATa                                                                             >  1:104229/1‑140 (MQ=255)                                                            aTTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATa                                                                             >  1:777980/1‑140 (MQ=255)                                                            aTTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATa                                                                             >  1:1195313/1‑140 (MQ=255)                                                             ttAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATaa                                                                            <  2:134536/140‑1 (MQ=255)                                                             ttAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATaa                                                                            <  1:1305522/140‑1 (MQ=255)                                                             ttAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGAAGATaa                                                                            <  2:107481/140‑1 (MQ=255)                                                                gagGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtg                                                                         >  2:796795/1‑140 (MQ=255)                                                                 agGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtgg                                                                        >  2:1510850/1‑140 (MQ=255)                                                                 agGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtgg                                                                        >  1:1414913/1‑140 (MQ=255)                                                                 agGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtgg                                                                        >  1:227293/1‑140 (MQ=255)                                                                   gggAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtggtg                                                                      >  1:822275/1‑140 (MQ=255)                                                                   gggAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAgtggtg                                                                      >  1:23607/1‑140 (MQ=255)                                                                     gAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGat                                                                    >  1:1366171/1‑140 (MQ=255)                                                                     aaaaaTGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGat                                                                    >  2:106079/2‑140 (MQ=255)                                                                      aaaaTGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGata                                                                   >  2:911142/1‑140 (MQ=255)                                                                        caTGTTAGCACAATTGCGACGTGAATCAAAAAGCCCGCCGCAATAAGCGAGCGTTAGTGTCAGCGCGCAACCAACACGGAGCACTCTGCGTGACGCACTACAGCTGCCGCGTTGCAACCGAGCAGATAAGAGGAGATATc                                                                 >  2:434052/2‑140 (MQ=255)                                                                           gTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCgg                                                              <  1:903739/140‑1 (MQ=255)                                                                            tCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGt                                                             <  1:877484/140‑1 (MQ=255)                                                                             cAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTc                                                            >  1:36436/1‑140 (MQ=255)                                                                              aGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCg                                                           <  2:1288147/140‑1 (MQ=255)                                                                              aGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCg                                                           >  1:1545911/1‑140 (MQ=255)                                                                                   aTTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATggg                                                      <  1:746682/140‑1 (MQ=255)                                                                                      tGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAg                                                   <  2:792199/140‑1 (MQ=255)                                                                                      tGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAg                                                   <  1:1339736/140‑1 (MQ=255)                                                                                      tGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAg                                                   <  2:74266/140‑1 (MQ=255)                                                                                         gAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCaa                                                <  2:1371465/140‑1 (MQ=255)                                                                                           aaTGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAatg                                              <  1:297181/140‑1 (MQ=255)                                                                                           aaTGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAatg                                              <  2:1508835/140‑1 (MQ=255)                                                                                             tGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAatgat                                            >  2:906199/1‑140 (MQ=255)                                                                                             tGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAatgat                                            <  1:960061/140‑1 (MQ=255)                                                                                             tGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAatgat                                            <  2:316504/140‑1 (MQ=255)                                                                                                 tCAAAAAGCCCGCATCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATc                                        <  1:724973/140‑1 (MQ=255)                                                                                                         cccGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGc                                >  1:560086/1‑140 (MQ=255)                                                                                                          ccGCAGCAATGTGCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCg                               <  1:818101/140‑1 (MQ=255)                                                                                                                   tgtgCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGAtcttc                      >  1:1381211/1‑140 (MQ=255)                                                                                                                      gCGGGCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCttcttc                   <  2:1366171/140‑1 (MQ=255)                                                                                                                        gggCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACACACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGc                 >  1:1127454/1‑140 (MQ=255)                                                                                                                         ggCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGcc                <  1:230192/140‑1 (MQ=255)                                                                                                                         ggCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGcc                <  2:1040366/140‑1 (MQ=255)                                                                                                                         ggCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGcc                <  2:148836/140‑1 (MQ=255)                                                                                                                          gCGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCa               >  1:1211972/1‑140 (MQ=255)                                                                                                                           cGTTAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCaa              <  1:289849/140‑1 (MQ=255)                                                                                                                              tAGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTc           <  2:147367/140‑1 (MQ=255)                                                                                                                               aGTGTCAGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTcc          >  2:649800/1‑140 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  1:95588/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  2:1010980/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  2:1036850/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  1:1218137/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  1:460963/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    >  2:891189/1‑140 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  1:781634/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  2:1351314/140‑1 (MQ=255)                                                                                                                                     aGCGCGCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATg    <  1:911142/140‑1 (MQ=255)                                                                                                                                       cgcgCAACCAGCGCGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCg  <  2:776437/140‑1 (MQ=255)                                                                                                                                       cgcgCAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCg  <  2:901512/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATGCCGGAACGCCCAGCATAAGAAATCTGATATAAAAAACTGTGGCGTGTATGGTACGGATTAGAGGGGAAAATGTCAGCACATTTGCGAAATGAATCAAAAAGCCCGCAGCAATGTGCGGGCGTTAGTGTCAGCGCACAACCAGCACGGAGCACTCTGCGTGACGCACTACAGCTGCGGCGTTGGAACCGAGCAGATAAGTGGTGATATCCGGTCGATGGGAAGCAATGATGATCATATGAGCGGGGATCTTCTTCGCCAATTCCAGAATGCG  >  NC_000913/1435055‑1435328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑