Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,606,990	A→G	intergenic (+15/‑9)	lsrF → / → lsrG	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase/(4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,606,990	0	A	G	100.0%	284.1 / NA	74	intergenic (+15/‑9)	lsrF/lsrG	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase/(4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/35);  total (39/35)

GGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGAGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACA  >  NC_000913/1606855‑1607123                                                                                                                                        |                                                                                                                                      ggggCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAAATCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGggaag                                                                                                                                   >  2:667109/1‑140 (MQ=255) ggggCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGGTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGggaag                                                                                                                                   >  1:1171673/1‑140 (MQ=255) ggggCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGGTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGggaag                                                                                                                                   >  1:427384/1‑140 (MQ=255) ggggCGTAATATTTTCCAGTCCGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGggaag                                                                                                                                   >  1:276885/1‑140 (MQ=255)   ggCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGgaagaa                                                                                                                                 <  2:1492078/140‑1 (MQ=255)       tAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTAt                                                                                                                             >  2:717581/1‑140 (MQ=255)       tAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTAt                                                                                                                             <  1:498200/140‑1 (MQ=255)       tAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTAt                                                                                                                             <  2:1115802/140‑1 (MQ=255)             tttCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGGAGAATTATGCACgg                                                                                                                       >  2:1005263/1‑139 (MQ=255)             tttCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGt                                                                                                                       <  2:354649/140‑1 (MQ=255)               tCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATTACGAAACGGCGGATCGGGCACATGAACTTTATCTGAGTGAAAAACAATAACTGCGGATCCAAGGGGAAAAATTATGCCCGTca                                                                                                                     >  2:614719/1‑140 (MQ=255)               tCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTca                                                                                                                     >  1:239493/1‑140 (MQ=255)               tCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTca                                                                                                                     >  1:129633/1‑140 (MQ=255)                      tGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGt                                                                                                              <  1:145650/140‑1 (MQ=255)                      tGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGt                                                                                                              <  1:282446/140‑1 (MQ=255)                               ggTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTaa                                                                                                     <  1:173854/140‑1 (MQ=255)                                 tGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACg                                                                                                   <  2:364042/140‑1 (MQ=255)                                      atgatgAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCAt                                                                                              <  1:677949/140‑1 (MQ=255)                                      atgatgAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCAt                                                                                              >  2:1085506/1‑140 (MQ=255)                                      atgatgAAAGCCGTACAGGCGGTGGGTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTCTCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGGAGAATTATGCACGGCACACTGGGTGAAATTAACGTTCAt                                                                                              >  2:1286384/1‑140 (MQ=255)                                             aaGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACa                                                                                       <  1:320468/140‑1 (MQ=255)                                                 cGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGt                                                                                   >  1:1228484/1‑140 (MQ=255)                                                   tACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTg                                                                                 >  1:1295842/1‑140 (MQ=255)                                                                  aCCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAg                                                                  >  1:794281/1‑140 (MQ=255)                                                                  aCCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAg                                                                  >  2:1600435/1‑140 (MQ=255)                                                                      tAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGtttt                                                              <  2:62208/140‑1 (MQ=255)                                                                         cGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCg                                                           >  2:1189503/1‑140 (MQ=255)                                                                         cGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCg                                                           >  2:409758/1‑140 (MQ=255)                                                                         cGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCg                                                           >  2:470996/1‑140 (MQ=255)                                                                             aCGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAg                                                       >  1:872357/1‑140 (MQ=255)                                                                              cGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGa                                                      <  1:322258/140‑1 (MQ=255)                                                                                gCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAac                                                    >  1:1260546/1‑140 (MQ=255)                                                                                gCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAac                                                    <  1:717581/140‑1 (MQ=255)                                                                                 cTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAacc                                                   >  1:1071395/1‑140 (MQ=255)                                                                                 cTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAacc                                                   >  1:734005/1‑140 (MQ=255)                                                                                  tGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAacca                                                  >  1:1153595/1‑140 (MQ=255)                                                                                     tCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCt                                               >  2:105557/1‑140 (MQ=255)                                                                                        ggCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTggg                                            <  2:239493/140‑1 (MQ=255)                                                                                        ggCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTggg                                            <  2:129633/140‑1 (MQ=255)                                                                                          cATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGct                                          <  1:678236/140‑1 (MQ=255)                                                                                          cATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGct                                          <  2:276885/140‑1 (MQ=255)                                                                                          cATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGct                                          <  1:1511749/140‑1 (MQ=255)                                                                                          cATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGct                                          >  2:1423282/1‑140 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  1:1259762/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  2:1295842/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  1:1421918/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         >  1:1356921/1‑140 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  2:427384/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  2:548846/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  1:369754/140‑1 (MQ=255)                                                                                           atatGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGctc                                         <  1:1075168/140‑1 (MQ=255)                                                                                                   tctATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAgg                                 >  2:1392928/1‑140 (MQ=255)                                                                                                   cctATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAgg                                 >  1:195284/2‑140 (MQ=255)                                                                                                     tATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGgaa                               >  1:1420827/1‑140 (MQ=255)                                                                                                       tCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGgaaga                             >  2:1406388/1‑140 (MQ=255)                                                                                                       tCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGgaaga                             >  2:1192203/1‑140 (MQ=255)                                                                                                       tCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGgaaga                             >  1:173480/1‑140 (MQ=255)                                                                                                       tCTGAGTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGgaaga                             >  2:1473669/1‑140 (MQ=255)                                                                                                            gTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCa                        >  2:318155/1‑140 (MQ=255)                                                                                                            gTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCa                        >  1:51543/1‑140 (MQ=255)                                                                                                            gTGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCa                        >  1:1037298/1‑140 (MQ=255)                                                                                                             tGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCaa                       >  2:1331707/1‑140 (MQ=255)                                                                                                             tGAAAAACAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCaa                       >  1:1552428/1‑140 (MQ=255)                                                                                                               aaaaaCAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAAtt                     >  1:930488/1‑140 (MQ=255)                                                                                                               aaaaaCAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAAtt                     >  1:967995/1‑140 (MQ=255)                                                                                                                 aaaCAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTg                   >  1:1367257/1‑140 (MQ=255)                                                                                                                   aCAGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTgcg                 <  2:1228484/140‑1 (MQ=255)                                                                                                                     aGTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCt               >  2:541724/1‑140 (MQ=255)                                                                                                                      gTAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCtt              <  1:1600435/140‑1 (MQ=255)                                                                                                                       tAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTc             >  1:1225618/1‑140 (MQ=255)                                                                                                                       tAACTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTc             <  1:1233312/140‑1 (MQ=255)                                                                                                                        aaCTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCg            <  2:1096674/140‑1 (MQ=255)                                                                                                                         aCTGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGa           <  1:1286384/140‑1 (MQ=255)                                                                                                                           tGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATg         <  2:734005/140‑1 (MQ=255)                                                                                                                           tGCGGATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATg         <  1:630341/140‑1 (MQ=255)                                                                                                                              ggATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCt      >  2:1033012/1‑140 (MQ=255)                                                                                                                               gATCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCtt     >  2:1529976/1‑140 (MQ=255)                                                                                                                                aTCTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTATACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTa    <  1:409758/140‑1 (MQ=255)                                                                                                                                  cTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACa  <  1:1473669/140‑1 (MQ=255)                                                                                                                                  cTAAGGGGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACa  <  2:1487377/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCGATGATGAAAGCCGTACAGGCGGTGGTTCACCATAACGAAACGGCTGATCGGGCATATGAACTCTATCTGAGTGAAAAACAGTAACTGCGGATCTAAGGAGAAGAATTATGCACGTCACACTGGTTGAAATTAACGTTCATGAAGACAAGGTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACA  >  NC_000913/1606855‑1607123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑