Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,647,973	T→C	Y14H (TAT→CAT)	dicA →	DNA‑binding transcriptional dual regulator DicA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,647,973	0	T	C	100.0%	313.2 / NA	83	Y14H (TAT→CAT)	dicA	DNA‑binding transcriptional dual regulator DicA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/33);  total (50/33)

TCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTAAAGTAT  >  NC_000913/1647837‑1648108                                                                                                                                         |                                                                                                                                        tCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCAtc                                                                                                                                      >  2:1566371/1‑140 (MQ=255)   aGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCAtcgt                                                                                                                                    <  2:171782/140‑1 (MQ=255)        tAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAGCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAGAAATTTAACTAGCGGCGAACGCATCAGGCATCGTCGGaa                                                                                                                               <  2:1357476/140‑1 (MQ=255)          aGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaaa                                                                                                                             <  1:290710/140‑1 (MQ=255)               ttAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTc                                                                                                                        >  2:1281796/1‑140 (MQ=255)               ttAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTc                                                                                                                        >  2:1398302/1‑140 (MQ=255)                tAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCa                                                                                                                       <  1:896977/140‑1 (MQ=255)                      acCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAacaca                                                                                                                 >  2:423671/1‑140 (MQ=255)                        cTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACAcc                                                                                                               >  1:727041/1‑140 (MQ=255)                        cTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACAcc                                                                                                               >  1:419374/1‑140 (MQ=255)                                 tAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGtct                                                                                                      <  1:401257/140‑1 (MQ=255)                                  aGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGGTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc                                                                                                     >  2:903994/1‑140 (MQ=255)                                  aGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc                                                                                                     >  2:1001503/1‑140 (MQ=255)                                  aGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc                                                                                                     >  2:1450720/1‑140 (MQ=255)                                    tCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCtt                                                                                                   >  1:1349158/1‑140 (MQ=255)                                    tCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCCGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGACCCtt                                                                                                   >  1:409661/1‑140 (MQ=255)                                       taactaacAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCt                                                                                                >  1:1482697/1‑140 (MQ=255)                                        aactaacAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTa                                                                                               <  1:1398302/140‑1 (MQ=255)                                        aactaacAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTa                                                                                               >  1:184734/1‑140 (MQ=255)                                          ctaacAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTaaa                                                                                             >  1:1328300/1‑140 (MQ=255)                                              cAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGccc                                                                                         >  1:1311061/1‑140 (MQ=255)                                              cAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGccc                                                                                         >  1:1562389/1‑140 (MQ=255)                                              cAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGccc                                                                                         >  2:848970/1‑140 (MQ=255)                                                  atagatGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa                                                                                     >  1:1080145/1‑140 (MQ=255)                                                  atagatGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa                                                                                     >  1:745665/1‑140 (MQ=255)                                                  atagatGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa                                                                                     >  2:1483594/1‑140 (MQ=255)                                                     gatGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAt                                                                                  >  1:1479428/1‑140 (MQ=255)                                                      atGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAtc                                                                                 <  2:798324/140‑1 (MQ=255)                                                          tAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTccc                                                                             <  1:1281796/140‑1 (MQ=255)                                                           aaCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCa                                                                            >  2:1300794/1‑140 (MQ=255)                                                            aCAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCAt                                                                           <  1:1566371/140‑1 (MQ=255)                                                                aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                                       >  1:520809/1‑140 (MQ=255)                                                                aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                                       <  1:1365290/140‑1 (MQ=255)                                                                aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                                       <  2:862314/140‑1 (MQ=255)                                                                aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                                       <  2:419374/140‑1 (MQ=255)                                                                aaaCATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATgtgt                                                                       >  2:1496285/1‑140 (MQ=255)                                                                   cATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTg                                                                    <  1:438136/140‑1 (MQ=255)                                                                      aGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTAt                                                                 >  2:1031427/1‑140 (MQ=255)                                                                      aGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTAt                                                                 <  2:975877/140‑1 (MQ=255)                                                                      aGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTAt                                                                 <  1:217991/140‑1 (MQ=255)                                                                        tCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATca                                                               >  1:770111/1‑140 (MQ=255)                                                                            tACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAAt                                                           >  2:1435035/1‑140 (MQ=255)                                                                                 tttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGaa                                                      >  1:1547236/1‑140 (MQ=255)                                                                                 tttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGaa                                                      >  1:639534/1‑140 (MQ=255)                                                                                 tttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGaa                                                      <  1:1300794/140‑1 (MQ=255)                                                                                  ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAAc                                                     >  2:435742/1‑140 (MQ=255)                                                                                  ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAAc                                                     <  1:377103/140‑1 (MQ=255)                                                                                  ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAAc                                                     >  2:152981/1‑140 (MQ=255)                                                                                    aGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgg                                                   >  1:1443850/1‑140 (MQ=255)                                                                                    aGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgg                                                   >  1:502984/1‑140 (MQ=255)                                                                                     gCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAA‑GTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgggg                                                 <  2:1441267/140‑1 (MQ=255)                                                                                      cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgggg                                                 >  1:668203/1‑140 (MQ=255)                                                                                      cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACgggg                                                 <  2:782692/140‑1 (MQ=255)                                                                                        ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTg                                               <  2:775970/140‑1 (MQ=255)                                                                                         tAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGa                                              >  1:1434819/1‑140 (MQ=255)                                                                                         tAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGa                                              >  2:484288/1‑140 (MQ=255)                                                                                          aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGAt                                             >  2:33216/1‑140 (MQ=255)                                                                                           ggtAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATa                                            <  1:903994/138‑1 (MQ=255)                                                                                               aCTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGa                                        <  2:1311061/140‑1 (MQ=255)                                                                                               aCTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGa                                        >  2:79548/1‑140 (MQ=255)                                                                                               aCTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGa                                        >  1:756481/1‑140 (MQ=255)                                                                                                 tATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAAc                                      <  2:1328300/140‑1 (MQ=255)                                                                                                  aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAAcc                                     >  1:74019/1‑140 (MQ=255)                                                                                                    ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTa                                   >  2:112937/1‑140 (MQ=255)                                                                                                     gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTAc                                  >  1:548004/1‑140 (MQ=255)                                                                                                     gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTAc                                  >  2:1338880/1‑140 (MQ=255)                                                                                                     gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTAc                                  >  2:231132/1‑140 (MQ=255)                                                                                                     gAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTAc                                  <  2:1080145/140‑1 (MQ=255)                                                                                                           aaaaaTTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTAGCTAAAGCCCCGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGgaa                            >  1:241331/1‑140 (MQ=255)                                                                                                                   aaCTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCttt                    >  2:63367/1‑140 (MQ=255)                                                                                                                     cTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCttttt                  <  1:791929/140‑1 (MQ=255)                                                                                                                        tCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGcc               >  2:554861/1‑140 (MQ=255)                                                                                                                        tCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGcc               <  2:380531/140‑1 (MQ=255)                                                                                                                             gAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAg          <  2:1579923/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  1:1496285/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  2:639534/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  2:745665/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  1:1435035/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  2:1547236/140‑1 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        >  2:153411/1‑140 (MQ=255)                                                                                                                               aCGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTa        <  1:1001503/140‑1 (MQ=255)                                                                                                                                  cATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTAAAg     >  2:539999/1‑140 (MQ=255)                                                                                                                                  cATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTAAAg     <  2:291376/140‑1 (MQ=255)                                                                                                                                     cAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTAAAGTAt  >  2:311013/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGTATCACAATGGGAACGGGGTGATAGTGAACCTACAGGGAAGAACCTTTTTGCCCTCAGTAAAGTAT  >  NC_000913/1647837‑1648108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑