Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,770,354	T→C	intergenic (+168/‑18)	ydiK → / → rprA	putative transporter YdiK/small regulatory RNA RprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,770,354	0	T	C	100.0%	157.8 / NA	42	intergenic (+168/‑18)	ydiK/rprA	putative transporter YdiK/small regulatory RNA RprA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

ACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGTATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGC  >  NC_000913/1770224‑1770490                                                                                                                                   |                                                                                                                                         accGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctact                                                                                                                                 >  1:1137847/1‑140 (MQ=255)  ccGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctacta                                                                                                                                <  1:638745/140‑1 (MQ=255)  ccGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCctacta                                                                                                                                >  1:616413/1‑140 (MQ=255)   cGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATTCCTACTAt                                                                                                                               >  1:1187394/1‑140 (MQ=255)   cGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTAt                                                                                                                               >  1:1583033/1‑140 (MQ=255)     cTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTa                                                                                                                             >  2:463300/1‑140 (MQ=255)     cTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTa                                                                                                                             >  2:934493/1‑140 (MQ=255)        aTTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCt                                                                                                                          <  1:224970/140‑1 (MQ=255)           gATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCAc                                                                                                                       <  2:632483/140‑1 (MQ=255)                   cttgctCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATaa                                                                                                               <  1:123243/140‑1 (MQ=255)                       ctctTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCa                                                                                                           >  2:407488/1‑140 (MQ=255)                       ctctTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCa                                                                                                           >  1:903764/1‑140 (MQ=255)                            ccccATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACata                                                                                                      >  2:1194575/1‑140 (MQ=255)                                 tatTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGAt                                                                                                 >  1:165761/1‑140 (MQ=255)                                    tttCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTta                                                                                              >  2:791057/1‑140 (MQ=255)                                     ttCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtat                                                                                             <  1:1222705/140‑1 (MQ=255)                                      tCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtata                                                                                            >  2:660739/1‑140 (MQ=255)                                      tCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTtata                                                                                            >  1:1356184/1‑140 (MQ=255)                                             tACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATgg                                                                                     >  1:1539014/1‑140 (MQ=255)                                             tACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATgg                                                                                     >  1:1260295/1‑140 (MQ=255)                                                             gtagtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGaa                                                                     >  1:521253/1‑140 (MQ=255)                                                             gtagtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGaa                                                                     >  1:987241/1‑140 (MQ=255)                                                                gtaCCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAaaca                                                                  <  1:1375526/140‑1 (MQ=255)                                                                       tCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAAtt                                                           <  2:1187394/140‑1 (MQ=255)                                                                       tCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAAtt                                                           >  2:383687/1‑140 (MQ=255)                                                                        cGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGTAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTg                                                          >  2:1313603/1‑140 (MQ=255)                                                                        cGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTg                                                          <  2:903764/140‑1 (MQ=255)                                                                         gCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGc                                                         >  2:371337/1‑140 (MQ=255)                                                                                cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  2:987241/140‑1 (MQ=255)                                                                                cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  1:934493/140‑1 (MQ=255)                                                                                cTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTgtgtgt                                                  <  1:463300/140‑1 (MQ=255)                                                                                         ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         <  2:521253/140‑1 (MQ=255)                                                                                         ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         >  1:62371/1‑140 (MQ=255)                                                                                         ttttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGc                                         >  1:187347/1‑140 (MQ=255)                                                                                          tttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGcc                                        <  1:407488/140‑1 (MQ=255)                                                                                          tttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGcc                                        <  1:1321748/140‑1 (MQ=255)                                                                                           ttAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGccc                                       <  2:391412/140‑1 (MQ=255)                                                                                                       gaCGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGAt                           <  2:616413/140‑1 (MQ=255)                                                                                                         cGAATCTGATCGACGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATgg                         >  2:229849/1‑140 (MQ=255)                                                                                                                      cGCAAAAAGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAAc            >  1:477830/1‑140 (MQ=255)                                                                                                                             aGTCCGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTc     <  2:187347/140‑1 (MQ=255)                                                                                                                                ccGCATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGc  <  2:1583033/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         ACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCCGTATGCCTACTATTAGCTCACGGTTATAAATCAACATATTGATTTATAAGCATGGAAATCCCCTGAGTGAAACAACGAATTGCTGTGTGTAGTCTTTGCCCATCTCCCACGATGGGCTTTTTTTTAACATTTTTCCGC  >  NC_000913/1770224‑1770490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑