Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,896	A→G	T210A (ACC→GCC)	nlpE →	lipoprotein NlpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,896	0	A	G	100.0%	293.3 / NA	78	T210A (ACC→GCC)	nlpE	lipoprotein NlpE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/40);  total (38/40)

ATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGA  >  NC_000913/215759‑216021                                                                                                                                          |                                                                                                                              aTTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGcc                                                                                                                             >  1:102876/1‑140 (MQ=255)   tGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCgg                                                                                                                           >  2:65807/1‑140 (MQ=255)    gcgcGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGt                                                                                                                          >  1:1004826/1‑140 (MQ=255)    gcgcGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGt                                                                                                                          <  1:1169406/140‑1 (MQ=255)    gcgcGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGt                                                                                                                          >  1:1515682/1‑140 (MQ=255)     cgcgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTg                                                                                                                         >  1:1067094/1‑140 (MQ=255)     cgcgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTg                                                                                                                         >  1:1276648/1‑140 (MQ=255)     cgcgACCGGAAAACGCTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTg                                                                                                                         >  2:156961/1‑140 (MQ=255)       cgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTgcg                                                                                                                       >  1:1082360/1‑140 (MQ=255)       cgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTgcg                                                                                                                       <  2:1308662/140‑1 (MQ=255)       cgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTgcg                                                                                                                       <  1:571939/140‑1 (MQ=255)                    tttCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTAt                                                                                                          <  1:647700/140‑1 (MQ=255)                    tttCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTAt                                                                                                          >  2:172698/1‑140 (MQ=255)                        aTGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGc                                                                                                      <  1:1366554/140‑1 (MQ=255)                         tGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCa                                                                                                     <  1:1409177/140‑1 (MQ=255)                         tGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCa                                                                                                     <  1:677532/140‑1 (MQ=255)                         tGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCa                                                                                                     <  2:894105/140‑1 (MQ=255)                           gTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCAcc                                                                                                   <  2:1518799/140‑1 (MQ=255)                            tAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCAccc                                                                                                  >  1:1172268/1‑140 (MQ=255)                                 aaTAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATAc                                                                                             >  1:980075/1‑140 (MQ=255)                                   tAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACgg                                                                                           <  2:948632/140‑1 (MQ=255)                                      cGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAg                                                                                        >  1:332010/1‑140 (MQ=255)                                      cGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAg                                                                                        >  2:135575/1‑140 (MQ=255)                                      cGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAg                                                                                        >  2:1208967/1‑140 (MQ=255)                                        cAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAggg                                                                                      <  1:479014/140‑1 (MQ=255)                                        cAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAggg                                                                                      >  1:1408224/1‑140 (MQ=255)                                        cAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAggg                                                                                      >  2:1559202/1‑140 (MQ=255)                                           agCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGaaa                                                                                   >  1:330473/1‑140 (MQ=255)                                                  gCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTAcc                                                                            <  1:156961/140‑1 (MQ=255)                                                  gCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTAcc                                                                            <  1:1536123/140‑1 (MQ=255)                                                  gCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTAcc                                                                            <  2:544215/140‑1 (MQ=255)                                                      aGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCaa                                                                        >  1:658451/1‑140 (MQ=255)                                                          aCCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAg                                                                    <  2:694862/140‑1 (MQ=255)                                                                cTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTTCCCCAACCAGGATTGc                                                              >  1:5688/1‑140 (MQ=255)                                                                cTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGc                                                              >  2:314169/1‑140 (MQ=255)                                                                cTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGc                                                              >  1:1380718/1‑140 (MQ=255)                                                                 tGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCa                                                             <  1:723332/140‑1 (MQ=255)                                                                  gcgcgcGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCCACCAGGATTGCag                                                            >  2:59318/1‑140 (MQ=255)                                                                  gcgcgcGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCag                                                            <  2:565116/140‑1 (MQ=255)                                                                  gcgcgcGGTCACAGTGAAAAACCGGTGTTACTGTCAGTACAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCag                                                            <  2:1272437/140‑1 (MQ=255)                                                                     cgcgGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGCAATCCGGATGCCAGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGGAAATTTTCTCCCCCCAGGATTTCggtag                                                         >  2:436730/1‑140 (MQ=255)                                                                            acaGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTgggg                                                  <  1:172698/140‑1 (MQ=255)                                                                                tGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGt                                              <  2:988977/140‑1 (MQ=255)                                                                                tGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGt                                              <  2:1466420/140‑1 (MQ=255)                                                                                  aaaaaCCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTaa                                            <  2:332010/140‑1 (MQ=255)                                                                                   aaaaCCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAAc                                           >  1:1467534/1‑140 (MQ=255)                                                                                    aaaCCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAAcc                                          >  2:1240620/1‑140 (MQ=255)                                                                                        cGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCCGGATTGCAGGAGGTTGGGGCAGTAACCCGTc                                      >  1:1184885/1‑140 (MQ=255)                                                                                         ggTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCt                                     <  1:135575/140‑1 (MQ=255)                                                                                          gtgtTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCATCCAGGATTGCGGAAGTTTGGGGCAGAAACCCGTCtt                                    >  1:18906/1‑140 (MQ=255)                                                                                          gtgtTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCCGTGGTTTGGGGCAGTAACCCGTCtt                                    >  1:31240/1‑140 (MQ=255)                                                                                          gtgtTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCtt                                    <  1:228886/140‑1 (MQ=255)                                                                                           ggtTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTg                                   <  2:1082360/139‑1 (MQ=255)                                                                                                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGaaac                         <  2:658451/140‑1 (MQ=255)                                                                                                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGaaac                         <  1:1350672/140‑1 (MQ=255)                                                                                                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGaaac                         >  2:1449466/1‑140 (MQ=255)                                                                                                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGaaac                         >  2:620817/1‑140 (MQ=255)                                                                                                     aGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGaaac                         >  2:1078155/1‑140 (MQ=255)                                                                                                          aaGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCATGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACg                    <  1:223692/140‑1 (MQ=255)                                                                                                          aaGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACg                    <  2:90521/140‑1 (MQ=255)                                                                                                           aGGTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGc                   >  2:37007/1‑140 (MQ=255)                                                                                                             gTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCaa                 >  1:1586076/1‑140 (MQ=255)                                                                                                             gTCACTTTACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCaa                 >  1:859766/1‑140 (MQ=255)                                                                                                                    tACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGc          <  2:1172268/140‑1 (MQ=255)                                                                                                                    tACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGc          <  2:350215/140‑1 (MQ=255)                                                                                                                    tACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGc          <  2:1515682/140‑1 (MQ=255)                                                                                                                    tACGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGc          <  2:980075/140‑1 (MQ=255)                                                                                                                     aCGCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGcc         <  1:242827/140‑1 (MQ=255)                                                                                                                       gCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCGCCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAg       <  2:1067094/140‑1 (MQ=255)                                                                                                                       gCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAg       <  2:816962/140‑1 (MQ=255)                                                                                                                       gCTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTACTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAg       <  1:1449466/140‑1 (MQ=255)                                                                                                                        cTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGa      <  2:102876/140‑1 (MQ=255)                                                                                                                        cTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGa      >  2:69550/1‑140 (MQ=255)                                                                                                                        cTTGAGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGa      <  1:1240620/140‑1 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGGAGTAACCCGGCTTGAGACAGAAACAAACGCAAAACAAGCCAGAAGGa  >  2:326566/1‑140 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  >  1:1304116/1‑140 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  >  1:231663/1‑140 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  >  1:1433194/1‑140 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  >  1:1466244/1‑140 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  <  2:1408224/140‑1 (MQ=255)                                                                                                                            aGGGTAATCCGGATGCCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGa  <  1:65807/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              ATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGAAGGTCACTTTACGCTTGAGGGTAATCCGGATACCGGTGCGCCGACTAAAGTATTGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGA  >  NC_000913/215759‑216021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑