Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,087	G→A	R157* (CGA→TGA)	wcaB ←	putative colanic acid biosynthesis acyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,087	0	G	A	100.0%	232.7 / NA	70	R157* (CGA→TGA)	wcaB	putative colanic acid biosynthesis acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/38);  total (32/38)

GTAACCGTAGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCA  >  NC_000913/2131954‑2132223                                                                                                                                      |                                                                                                                                         gTAACCGTAGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCttt                                                                                                                                    <  2:1140616/140‑1 (MQ=255)   aaCCGTAGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCttttt                                                                                                                                  >  1:83978/1‑140 (MQ=255)        tAGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTccc                                                                                                                             >  2:883320/1‑140 (MQ=255)         aGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCcca                                                                                                                            >  1:1442808/1‑140 (MQ=255)               aaTGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCccaccacca                                                                                                                      <  2:1460861/140‑1 (MQ=255)               aaTGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCccaccacca                                                                                                                      >  1:1063507/1‑140 (MQ=255)               aaTGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCccaccacca                                                                                                                      <  2:1349238/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  2:1388242/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  2:151055/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  2:174234/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  1:956125/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  2:777828/140‑1 (MQ=255)                    gACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGcgcg                                                                                                                 <  1:496889/140‑1 (MQ=255)                         cAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTc                                                                                                            <  2:1554933/140‑1 (MQ=255)                         cAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTc                                                                                                            <  1:72714/140‑1 (MQ=255)                           gCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCg                                                                                                          >  1:1063121/1‑140 (MQ=255)                            ccccTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCgg                                                                                                         <  2:1062641/140‑1 (MQ=255)                            ccccTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCgg                                                                                                         >  1:1470978/1‑140 (MQ=255)                             cccTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCggg                                                                                                        <  1:352791/140‑1 (MQ=255)                                tGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACa                                                                                                     <  1:415972/140‑1 (MQ=255)                                tGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACa                                                                                                     <  1:1052405/140‑1 (MQ=255)                                tGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACa                                                                                                     <  2:303559/140‑1 (MQ=255)                                   tGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACagag                                                                                                  >  1:1118297/1‑140 (MQ=255)                                   tGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACagag                                                                                                  <  1:470595/140‑1 (MQ=255)                                       gcgcACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGa                                                                                              >  1:557581/1‑140 (MQ=255)                                        cgcACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAg                                                                                             >  1:1568444/1‑140 (MQ=255)                                        cgcACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAg                                                                                             >  1:1086762/1‑140 (MQ=255)                                        cgcACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAg                                                                                             >  2:1586922/1‑140 (MQ=255)                                          cACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGca                                                                                           >  2:925562/1‑140 (MQ=255)                                               tggtggAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACg                                                                                      >  1:1204287/1‑140 (MQ=255)                                                       aTCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCcgc                                                                              >  2:458981/1‑140 (MQ=255)                                                        tCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCcgcg                                                                             <  1:458981/140‑1 (MQ=255)                                                         cTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCgcgc                                                                            >  1:825192/1‑140 (MQ=255)                                                         cTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCgcgc                                                                            >  2:702117/1‑140 (MQ=255)                                                         cTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCgcgc                                                                            >  2:572861/1‑140 (MQ=255)                                                          tAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCTTTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGcc                                                                           <  1:216161/140‑1 (MQ=255)                                                                 acacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTg                                                                    >  2:455165/1‑140 (MQ=255)                                                                 acacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTg                                                                    <  2:1470978/140‑1 (MQ=255)                                                                  cacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGa                                                                   <  2:83978/140‑1 (MQ=255)                                                                  cacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGa                                                                   <  2:787932/140‑1 (MQ=255)                                                                  cacCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGa                                                                   >  1:1347674/1‑140 (MQ=255)                                                                   acCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGAc                                                                  >  2:377577/1‑140 (MQ=255)                                                                   acCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGCCGAGCACCACGCTGCCCGCGCCCACGGTGAc                                                                  >  1:405991/1‑140 (MQ=255)                                                                      tgctgcCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACgtt                                                               <  2:1063121/140‑1 (MQ=255)                                                                        ctgcCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACgttgt                                                             >  2:1549623/1‑140 (MQ=255)                                                                        ctgcCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACAACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCTCACGCTCACGGGGCCgatgt                                                             >  2:1412755/1‑140 (MQ=255)                                                                          gcCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTa                                                           <  1:191181/140‑1 (MQ=255)                                                                           ccccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTAc                                                          >  1:148452/1‑140 (MQ=255)                                                                           ccccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTAc                                                          >  2:266069/1‑140 (MQ=255)                                                                           ccccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTAc                                                          >  1:866357/1‑140 (MQ=255)                                                                           ccccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTAc                                                          >  2:575956/1‑140 (MQ=255)                                                                            cccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTAcc                                                         <  1:986020/140‑1 (MQ=255)                                                                             ccGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCa                                                        >  1:182103/1‑140 (MQ=255)                                                                                ccTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGc                                                     <  2:557581/140‑1 (MQ=255)                                                                                 cTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCg                                                    <  1:572861/140‑1 (MQ=255)                                                                                    ccGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGa                                                 <  1:1428750/140‑1 (MQ=255)                                                                                     cGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGat                                                >  2:984183/1‑140 (MQ=255)                                                                                     cGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGat                                                >  1:744958/1‑140 (MQ=255)                                                                                         aGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCa                                            <  2:1347674/140‑1 (MQ=255)                                                                                          gTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGACCACGGTGACGTTGTTACCAAGCGTGATATCAc                                           >  1:792422/1‑140 (MQ=255)                                                                                           tCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGCGACGTTGTTACCAAGCGTGATATCAcc                                          >  1:378330/1‑140 (MQ=255)                                                                                             gCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCaa                                        <  1:1586922/140‑1 (MQ=255)                                                                                                  ttAAATTGCAAAATATTCATTTAATTGCCTTCTCTCACGCTTTTTCGCCCTCCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGaata                                   <  2:1568444/140‑1 (MQ=255)                                                                                                      aTTGCAAAATATTCATTTAATTACCTTCACTCACGCTTTTTCTCTCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGa                               <  2:793407/140‑1 (MQ=255)                                                                                                            aaaTATTCATTTAATTACCTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTgg                         <  2:353021/140‑1 (MQ=255)                                                                                                                             ccTTCACTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCg        <  2:944179/140‑1 (MQ=255)                                                                                                                                tcactcacGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTg     >  1:153018/1‑140 (MQ=255)                                                                                                                                  ccTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGcc   <  2:1166031/139‑1 (MQ=255)                                                                                                                                  aCTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGcc   <  1:984183/140‑1 (MQ=255)                                                                                                                                  aCTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGcc   <  2:825192/140‑1 (MQ=255)                                                                                                                                  aCTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGcc   <  2:1555315/140‑1 (MQ=255)                                                                                                                                   cTCACGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCa  <  2:656031/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GTAACCGTAGACAAAATGTGACGCCAGCCCCTGTTGCAGCGCACGCTGGTGGAGATCTAACGCCACACCTGCTGCCCCGCCTTCCGCCAGTCGCACATTAAATTGCAAAATATTCATTTAATTACCTTCACTCGCGCTTTTTCTCCCACCACCAGCGCGTTGTCCGGGACAGAGTCGAGCACCACGCTGCCCGCGCCCACGGTGACGTTGTTACCAAGCGTGATATCACCAAGAATAATGACGTTGGCACCGAGTTCGACGCCGTTGCCA  >  NC_000913/2131954‑2132223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑