Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,159,983	T→C	L533P (CTG→CCG)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,159,983	0	T	C	100.0%	257.7 / NA	67	L533P (CTG→CCG)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/43);  total (24/43)

CTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAG  >  NC_000913/2159846‑2160119                                                                                                                                          |                                                                                                                                         cTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGtgccgc                                                                                                                                        >  1:524210/1‑140 (MQ=255)    gATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTg                                                                                                                                     >  1:956707/1‑140 (MQ=255)     aTGCTGAAAGCCAGCAAGGCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATCCCCGTCTGGTGGGCGTGGTGCCGCTTgg                                                                                                                                    <  2:301737/140‑1 (MQ=255)      tGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGc                                                                                                                                   >  2:976732/1‑140 (MQ=255)          gAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCa                                                                                                                               <  1:752423/140‑1 (MQ=255)          gAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCa                                                                                                                               <  2:693188/140‑1 (MQ=255)          gAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCa                                                                                                                               <  1:634913/140‑1 (MQ=255)             aGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTg                                                                                                                            <  2:357661/140‑1 (MQ=255)               ccAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTgcg                                                                                                                          <  1:370870/140‑1 (MQ=255)               ccAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTgcg                                                                                                                          <  1:92731/140‑1 (MQ=255)               ccAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTgcg                                                                                                                          <  2:1424749/140‑1 (MQ=255)                      gCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAata                                                                                                                   >  2:1507104/1‑140 (MQ=255)                       ccGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAatat                                                                                                                  <  1:999200/140‑1 (MQ=255)                         gcgcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCt                                                                                                                >  2:361036/1‑140 (MQ=255)                          cgcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTg                                                                                                               >  2:787310/1‑140 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  1:203470/140‑1 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  1:665572/140‑1 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  2:524210/140‑1 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  1:737774/140‑1 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  1:771346/140‑1 (MQ=255)                           gcgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTgg                                                                                                              <  1:599655/140‑1 (MQ=255)                            cgAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGc                                                                                                             <  1:516392/140‑1 (MQ=255)                             gAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCt                                                                                                            >  1:1349409/1‑140 (MQ=255)                                         cTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGAt                                                                                                >  1:391072/1‑140 (MQ=255)                                                  tttGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAAc                                                                                       <  1:1170807/140‑1 (MQ=255)                                                     ggTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACctt                                                                                    <  1:275240/140‑1 (MQ=255)                                                          cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTcc                                                                               <  1:71584/140‑1 (MQ=255)                                                          cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTcc                                                                               <  1:178726/140‑1 (MQ=255)                                                          cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTcc                                                                               <  1:1265084/140‑1 (MQ=255)                                                          cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTcc                                                                               <  1:787310/140‑1 (MQ=255)                                                          cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTACTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTcc                                                                               >  1:755155/1‑140 (MQ=255)                                                               tGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAg                                                                          <  2:1221304/140‑1 (MQ=255)                                                                 gTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCa                                                                        <  1:1507104/140‑1 (MQ=255)                                                                      ccTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGaca                                                                   <  1:501104/140‑1 (MQ=255)                                                                      ccTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGaca                                                                   <  1:280105/140‑1 (MQ=255)                                                                         gCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTg                                                                <  1:609905/140‑1 (MQ=255)                                                                          caacaaGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTgg                                                               >  2:831144/1‑140 (MQ=255)                                                                          caacaaGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTgg                                                               >  2:920512/1‑140 (MQ=255)                                                                                 gCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCTCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGCCACTGGCGTGTTg                                                        >  1:722006/1‑140 (MQ=255)                                                                                  cTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGa                                                       <  1:760651/140‑1 (MQ=255)                                                                                  cTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGa                                                       >  2:598802/1‑140 (MQ=255)                                                                                     cGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATgg                                                    >  1:1195351/1‑140 (MQ=255)                                                                                      ggCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATggg                                                   <  1:524391/140‑1 (MQ=255)                                                                                      ggCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATggg                                                   <  2:1559073/140‑1 (MQ=255)                                                                                           gTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGa                                              >  2:1043994/1‑140 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  2:956707/140‑1 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  1:1476795/140‑1 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  2:604034/140‑1 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  1:652000/140‑1 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  1:897260/140‑1 (MQ=255)                                                                                                 aaaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAgg                                        <  2:350962/140‑1 (MQ=255)                                                                                                  aaaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGc                                       <  2:134791/140‑1 (MQ=255)                                                                                                   aaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCg                                      <  1:920512/140‑1 (MQ=255)                                                                                                       ggTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATc                                  >  2:418716/1‑140 (MQ=255)                                                                                                        gtacTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCa                                 <  2:755155/137‑1 (MQ=255)                                                                                                        gTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCa                                 >  2:1104387/1‑140 (MQ=255)                                                                                                                     cccGTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGttt                    <  2:961703/140‑1 (MQ=255)                                                                                                                        gTCTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGTGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAg                 >  1:1345390/1‑140 (MQ=255)                                                                                                                          cTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAggt               >  1:876311/1‑139 (MQ=255)                                                                                                                          cTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGc               >  2:1585924/1‑140 (MQ=255)                                                                                                                          cTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGc               >  2:886746/1‑140 (MQ=255)                                                                                                                          cTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGc               >  2:1405619/1‑140 (MQ=255)                                                                                                                          cTGGTGGGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGc               >  2:191779/1‑140 (MQ=255)                                                                                                                            ggtggGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGa             <  2:391072/140‑1 (MQ=255)                                                                                                                             gtggGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGAt            >  2:608079/1‑140 (MQ=255)                                                                                                                             gtggGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGAt            >  1:265976/1‑140 (MQ=255)                                                                                                                             gtggGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGAt            <  2:120314/140‑1 (MQ=255)                                                                                                                              tggGCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATg           <  1:46120/140‑1 (MQ=255)                                                                                                                               gggCGTGGTGCCGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATgc          <  2:722006/140‑1 (MQ=255)                                                                                                                                       tgccgcTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAg  <  1:582726/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAG  >  NC_000913/2159846‑2160119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑