Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,343	T→C	L415P (CTG→CCG)	yehQ →	SWIM zinc finger domains‑containing protein YehQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,343	0	T	C	100.0%	193.1 / NA	52	L415P (CTG→CCG)	yehQ	SWIM zinc finger domains‑containing protein YehQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/37);  total (15/37)

TTCGCGCAGTTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGC  >  NC_000913/2210209‑2210475                                                                                                                                       |                                                                                                                                     ttCGCGCAGTTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGt                                                                                                                                 >  1:826901/1‑140 (MQ=255)    gcgcAGTTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAg                                                                                                                              <  2:753393/140‑1 (MQ=255)         gTTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTAcc                                                                                                                         >  1:69798/1‑140 (MQ=255)             gCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCa                                                                                                                     <  1:1563357/140‑1 (MQ=255)               cgcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAAc                                                                                                                   <  1:659767/140‑1 (MQ=255)               cgcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAAc                                                                                                                   <  1:1513508/140‑1 (MQ=255)                  gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGc                                                                                                                <  1:1228755/140‑1 (MQ=255)                  gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGc                                                                                                                <  1:721778/140‑1 (MQ=255)                  gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGc                                                                                                                <  2:302840/140‑1 (MQ=255)                    aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCCACCGcct                                                                                                              >  2:261063/1‑138 (MQ=255)                       gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAag                                                                                                           <  2:209055/140‑1 (MQ=255)                       gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAag                                                                                                           <  2:175000/140‑1 (MQ=255)                             gAAACCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                                                                                                     <  2:1372203/140‑1 (MQ=255)                              aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                                                                                                    >  1:900350/1‑140 (MQ=255)                                 aCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGTAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                                                                                                 <  2:1177406/140‑1 (MQ=255)                                 aCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                                                                                                 <  1:599529/140‑1 (MQ=255)                                 aCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                                                                                                 <  1:809535/140‑1 (MQ=255)                                  cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                                                                                                <  2:342095/140‑1 (MQ=255)                                    ccccGGCAGGTGCCAGTCGGCTGTTTGGTTTTCGGGCCGGCGTCCTGGGGGGCGGGAAAATTGTTTCACAAGCAGGAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTTCCCGCAAACGCTTAAGCAGCGTTGTGCCg                                                                                              <  2:886384/138‑1 (MQ=255)                                    cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCg                                                                                              >  1:159307/1‑140 (MQ=255)                                    cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCg                                                                                              <  2:1324038/140‑1 (MQ=255)                                      ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCt                                                                                            <  2:52496/140‑1 (MQ=255)                                               aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa                                                                                   <  1:1514330/140‑1 (MQ=255)                                                 gcgTCGGCTGTTTAGTTTGCAGGCTGGCGCACGGGCGGGCGGGCAAATTGTTTCACAGGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg                                                                                 <  2:9780/140‑1 (MQ=255)                                                  cgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc                                                                                <  2:840703/140‑1 (MQ=255)                                                       gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTggg                                                                           >  1:833100/1‑139 (MQ=255)                                                       gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGc                                                                           >  2:775098/1‑140 (MQ=255)                                                                  ttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAgg                                                                >  2:391817/1‑139 (MQ=255)                                                                   tCAGGCTGGGGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAgcg                                                               <  1:346007/140‑1 (MQ=255)                                                                   tCAGGCTGGCGGAATGGTGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAgcg                                                               <  2:739526/140‑1 (MQ=255)                                                                   tCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAgcg                                                               <  1:136528/140‑1 (MQ=255)                                                                    cAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGGCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAgcgc                                                              <  2:1189265/140‑1 (MQ=255)                                                                                   gcgggcggGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCCATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGcc                                               >  2:67956/1‑140 (MQ=255)                                                                                    cgggcgggCAAATTGTTTCACAAGCAGCAAAGCGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCg                                              <  2:733589/140‑1 (MQ=255)                                                                                     gggcgggcAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCgg                                             >  1:1113419/1‑140 (MQ=255)                                                                                      ggcgggcAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCggg                                            <  2:1429841/140‑1 (MQ=255)                                                                                           gtaaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATtg                                       >  1:1505904/3‑140 (MQ=255)                                                                                           gcAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATtg                                       >  2:193023/1‑140 (MQ=255)                                                                                           gcAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATtg                                       >  1:1441070/1‑140 (MQ=255)                                                                                              aaTTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTgg                                    <  2:826901/140‑1 (MQ=255)                                                                                                   tttCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTg                               <  1:1482339/140‑1 (MQ=255)                                                                                                   tttCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTg                               <  2:1126828/140‑1 (MQ=255)                                                                                                   tttCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTg                               <  2:972214/140‑1 (MQ=255)                                                                                                    ttCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGc                              <  1:775098/140‑1 (MQ=255)                                                                                                        caAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGa                          <  2:376685/140‑1 (MQ=255)                                                                                                        caAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGa                          <  2:525569/140‑1 (MQ=255)                                                                                                        caAGCAGCAAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGa                          <  1:736267/140‑1 (MQ=255)                                                                                                              gcaAAACGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATAttt                    >  1:1556796/1‑140 (MQ=255)                                                                                                                  aaCGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGc                <  1:1554249/140‑1 (MQ=255)                                                                                                                   aCGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGcc               <  1:444009/140‑1 (MQ=255)                                                                                                                    cGCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCa              <  2:1441070/140‑1 (MQ=255)                                                                                                                     gCAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAg             >  2:1099064/1‑140 (MQ=255)                                                                                                                      cAGTGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGc            <  1:1421074/140‑1 (MQ=255)                                                                                                                         tGCCGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTc         >  1:829608/1‑140 (MQ=255)                                                                                                                           ccGATGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTccc       <  1:67956/140‑1 (MQ=255)                                                                                                                             gagggCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGAAAATGTTGGGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTccccc     <  2:1166011/137‑1 (MQ=255)                                                                                                                              aTGGCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTcccccc    >  2:1503830/1‑140 (MQ=255)                                                                                                                                ggCGAGCCGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGc  <  2:618182/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TTCGCGCAGTTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGC  >  NC_000913/2210209‑2210475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑