Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,239,585	A→G	V255A (GTG→GCG)	mglB ←	D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,239,585	0	A	G	100.0%	362.8 / NA	94	V255A (GTG→GCG)	mglB	D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/38);  total (56/38)

CACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTG  >  NC_000913/2239448‑2239721                                                                                                                                          |                                                                                                                                         cACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGcc                                                                                                                                        >  2:1129519/1‑140 (MQ=255) cACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGcc                                                                                                                                        >  2:1520485/1‑140 (MQ=255)  ccttttCCCATCGGCCGGTTTTTTCGCCAGATAAAGGGTCGTTTTCGCGTGGTTGTAAGCATCGTTCAGTGGGGGGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCg                                                                                                                                       <  2:351417/137‑1 (MQ=255)  ccttttCCCATCGGCAAGGTTTTTCGCCAGAACAAGGGTCGCTTTCGCCTGGTTGTTACCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCg                                                                                                                                       <  1:1376312/137‑1 (MQ=255)  cccTTTACCATGGGCCATTTTTTGCGCCAGACCAAAGGCCGCTTTCCCCTGGTTGTAAGCATCGTTCAGTACGGGGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCg                                                                                                                                       <  2:1394123/139‑1 (MQ=255)      ttACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAAt                                                                                                                                   >  2:881050/1‑140 (MQ=255)        cccATCGGCCGGGTTTTTCGCCAGATCAAAGGTCGTTTCCCCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGc                                                                                                                                 <  2:1495703/139‑1 (MQ=255)            tCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGa                                                                                                                             >  2:362306/1‑140 (MQ=255)             cGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGAc                                                                                                                            >  2:800263/1‑140 (MQ=255)             cGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGAc                                                                                                                            <  2:406784/140‑1 (MQ=255)              ggCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACt                                                                                                                           >  1:1254270/1‑140 (MQ=255)              ggCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACt                                                                                                                           >  1:1590283/1‑140 (MQ=255)                ccAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACttg                                                                                                                         >  1:200175/1‑140 (MQ=255)                 cgggTTTTTCCCCAAAAAAAAGGTCGCTTCCGCCTGGTTGTTAGCATCGTTCAGTCCGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACttgt                                                                                                                        <  2:125850/138‑1 (MQ=255)                   ggTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACttgttg                                                                                                                      >  1:1130218/1‑140 (MQ=255)                   ggTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACttgttg                                                                                                                      >  1:1160734/1‑140 (MQ=255)                       ttccGCCAGATAAAAGGCCGCTCTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTtgtgtg                                                                                                                  <  2:55959/137‑1 (MQ=255)                            ccAGAACAAGGGTCGTTTTCGCCTGTTTGTGATCATTTTTCAGTACGTCGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTc                                                                                                             <  1:323289/140‑1 (MQ=255)                            caaGACCAAGGGCCGTTTTCGCCTGGTTGTTAGCATCGTCCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTc                                                                                                             <  2:1004028/138‑1 (MQ=255)                                aTCAAGGGTCGCTTTCGCCGGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAgcg                                                                                                         <  1:484720/140‑1 (MQ=255)                                aTCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAgcg                                                                                                         >  1:869022/1‑140 (MQ=255)                                   aagggTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCtt                                                                                                      <  2:534550/137‑1 (MQ=255)                                    aaGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTc                                                                                                     >  1:714314/1‑140 (MQ=255)                                         cGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcg                                                                                                >  1:74557/1‑140 (MQ=255)                                         cGCTTGCGCCTGGTTGTTAGAGTCTTTCAAAAGGGGCCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcg                                                                                                <  2:585510/140‑1 (MQ=255)                                          gCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcgc                                                                                               >  1:1487956/1‑140 (MQ=255)                                           ttttCGCCGGGTTGTTAGCATCGTTCAGTGGGGGGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcgcg                                                                                              <  2:954187/139‑1 (MQ=255)                                           cTTTCGCTTGTTTGTTAGCACCGTTCAGTACGTTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcgcg                                                                                              <  2:523612/140‑1 (MQ=255)                                           cTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcgcg                                                                                              >  1:177814/1‑140 (MQ=255)                                           cTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACcgcg                                                                                              >  1:1002796/1‑140 (MQ=255)                                               cGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCa                                                                                          >  2:673102/1‑140 (MQ=255)                                                     tttgttAGCATCGTCCAGTCCGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCca                                                                                    <  2:1556417/139‑1 (MQ=255)                                                      ttgttAGCATCGTTCAGTCCGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCcat                                                                                   <  1:270455/140‑1 (MQ=255)                                                          tAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCcatcgca                                                                               <  2:1561709/140‑1 (MQ=255)                                                           aGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCcatcgcat                                                                              >  1:311315/1‑140 (MQ=255)                                                            gCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCcatcgcatc                                                                             >  2:553298/1‑140 (MQ=255)                                                             cATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCcatcgcatcg                                                                            <  2:1250085/140‑1 (MQ=255)                                                               tCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgtt                                                                          >  2:839099/1‑140 (MQ=255)                                                               tCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgtt                                                                          >  1:1555867/1‑140 (MQ=255)                                                               tCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgtt                                                                          >  2:1377170/1‑140 (MQ=255)                                                               tCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgtt                                                                          >  2:828278/1‑140 (MQ=255)                                                                cGTTCAGTAGGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttg                                                                         <  2:1140640/140‑1 (MQ=255)                                                                cGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttg                                                                         >  2:156466/1‑140 (MQ=255)                                                                cGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttg                                                                         <  1:335688/140‑1 (MQ=255)                                                                cGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttg                                                                         >  2:1005522/1‑140 (MQ=255)                                                                cGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttg                                                                         >  2:662808/1‑140 (MQ=255)                                                                 gTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttgt                                                                        <  2:405578/140‑1 (MQ=255)                                                                  ttCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttgtt                                                                       >  2:705990/1‑140 (MQ=255)                                                                  ttCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCgttgtt                                                                       >  2:104040/1‑140 (MQ=255)                                                                     aGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGc                                                                    >  1:625184/1‑140 (MQ=255)                                                                          ggTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATaa                                                               >  2:1142402/1‑140 (MQ=255)                                                                          ggTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATaa                                                               >  1:1278356/1‑140 (MQ=255)                                                                           gTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAACGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAc                                                              <  1:60676/140‑1 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  2:65930/1‑140 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  1:285818/1‑140 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  2:755142/1‑140 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  2:100880/1‑140 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  1:759693/1‑140 (MQ=255)                                                                            tGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAAcc                                                             >  2:1551697/1‑140 (MQ=255)                                                                              cccGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCAc                                                           >  2:1433554/1‑140 (MQ=255)                                                                              cccGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCAc                                                           >  1:707581/1‑140 (MQ=255)                                                                                 gCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTc                                                        >  2:463768/1‑140 (MQ=255)                                                                                 gCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTc                                                        >  1:171844/1‑140 (MQ=255)                                                                                 gCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTc                                                        >  1:1433724/1‑140 (MQ=255)                                                                                 gCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTc                                                        >  1:688979/1‑140 (MQ=255)                                                                                      tGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGAttt                                                   >  2:1562554/1‑140 (MQ=255)                                                                                          ccGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTttgtt                                               <  2:1590283/140‑1 (MQ=255)                                                                                           cGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTttgttg                                              <  1:1166572/140‑1 (MQ=255)                                                                                           cGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTttgttg                                              <  2:1216887/140‑1 (MQ=255)                                                                                             gATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGc                                            >  1:1323804/1‑140 (MQ=255)                                                                                               tttCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGt                                          <  2:864803/140‑1 (MQ=255)                                                                                                ttCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGtt                                         >  2:571392/1‑140 (MQ=255)                                                                                                 tCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTc                                        <  1:1289223/140‑1 (MQ=255)                                                                                                     cagcgccagcgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGc                                    >  2:936570/1‑140 (MQ=255)                                                                                                       gcgccagcgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCa                                  >  2:1343934/1‑140 (MQ=255)                                                                                                        cgccagcgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAg                                 >  2:1436954/1‑140 (MQ=255)                                                                                                            agcgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAg                             >  2:1243574/1‑140 (MQ=255)                                                                                                             gcgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGc                            <  2:285818/140‑1 (MQ=255)                                                                                                              cgcTTCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGcc                           <  1:1494481/140‑1 (MQ=255)                                                                                                                 ttCTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAgg                        <  2:200175/140‑1 (MQ=255)                                                                                                                   cTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCg                      >  2:347903/1‑140 (MQ=255)                                                                                                                   cTGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCg                      <  1:65930/140‑1 (MQ=255)                                                                                                                    tGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGt                     <  1:103497/140‑1 (MQ=255)                                                                                                                    tGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGt                     <  2:1386461/140‑1 (MQ=255)                                                                                                                    tGGCAGCGCATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGt                     <  1:1553379/140‑1 (MQ=255)                                                                                                                          cgcATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatct               >  2:328177/1‑140 (MQ=255)                                                                                                                          cgcATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatct               <  2:999876/140‑1 (MQ=255)                                                                                                                           gcATCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatctt              >  1:756061/1‑140 (MQ=255)                                                                                                                             aTCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatcttat            >  2:1521022/1‑140 (MQ=255)                                                                                                                             aTCGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatcttat            >  1:386389/1‑140 (MQ=255)                                                                                                                               cGACGCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCatcttatct          <  2:1262058/140‑1 (MQ=255)                                                                                                                                 gcGCCAAACGCCGGAATGCTGGCCTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCttt        <  2:1403614/139‑1 (MQ=255)                                                                                                                                   gCCAAACGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGTGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCg      >  1:90969/1‑140 (MQ=255)                                                                                                                                      aaaCGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCt   <  2:492593/140‑1 (MQ=255)                                                                                                                                       aaCGCCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTg  <  1:662808/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CACCTTTACCATCGGCCAGGTTTTTCGCCAGATCAAAGGTCGCTTTCGCCTGGTTGTTAGCATCGTTCAGTACGGTGCCCGCCAGTGCACCGGATTTCACCAGCGCCAGCGCTTCTGGCAGCGCATCGACGCCAAACACCGGAATGCTGGACTTGTTGTGTGCTTTCAGCGCTTCAACCGCGCCCATTGCCATCGCATCGTTGTTGGCGATAACCACTTCGATTTTGTTGGCGTTCGGGCCAGACAGCCAGGCGTCCATCTTATCTTTCGCCTG  >  NC_000913/2239448‑2239721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑