Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,538	G→A	P87L (CCG→CTG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,538	0	G	A	100.0%	255.8 / NA	73	P87L (CCG→CTG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/37);  total (36/37)

TTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTG  >  NC_000913/2329408‑2329673                                                                                                                                   |                                                                                                                                        ttGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTc                                                                                                                                >  1:1370256/1‑140 (MQ=255)   gCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCGTCCAGGGAGTTGCcg                                                                                                                              <  2:60226/140‑1 (MQ=255)   gCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAACCTGACAACAATAAACCTCTTCCAGGGAGTTTCcg                                                                                                                              <  2:1566577/140‑1 (MQ=255)      aGTATTGCCCCCACACACCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCGCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGa                                                                                                                           <  1:410773/140‑1 (MQ=255)        tATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGaca                                                                                                                         <  1:332214/140‑1 (MQ=255)        tATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGaca                                                                                                                         <  1:159911/140‑1 (MQ=255)           tGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTg                                                                                                                      >  1:851291/1‑140 (MQ=255)           tGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTg                                                                                                                      >  1:979723/1‑140 (MQ=255)             cccccACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGt                                                                                                                    >  1:739555/1‑140 (MQ=255)             cccccACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGt                                                                                                                    >  1:149977/1‑140 (MQ=255)                  acacaTCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGgc                                                                                                               >  2:990537/1‑140 (MQ=255)                  acacaTCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGgc                                                                                                               >  1:176818/1‑140 (MQ=255)                       tCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTc                                                                                                          <  2:720785/140‑1 (MQ=255)                          gcgcCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCgg                                                                                                       <  1:845439/140‑1 (MQ=255)                           cgcCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGt                                                                                                      >  2:506277/1‑140 (MQ=255)                           cgcCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGt                                                                                                      >  1:1348984/1‑140 (MQ=255)                           cgcCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGt                                                                                                      >  1:512270/1‑140 (MQ=255)                             ccAATATGCAGCCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTg                                                                                                    <  2:582183/140‑1 (MQ=255)                                   tGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGt                                                                                              <  2:1414337/140‑1 (MQ=255)                                   tGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGt                                                                                              >  1:783801/1‑140 (MQ=255)                                   tGCAACCGCTGCAGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGt                                                                                              <  1:585236/140‑1 (MQ=255)                                    gCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTa                                                                                             <  1:758866/140‑1 (MQ=255)                                      aaCCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTAtc                                                                                           >  2:1418171/1‑140 (MQ=255)                                        ccGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTAtcct                                                                                         <  2:1302473/140‑1 (MQ=255)                                        ccGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTAtcct                                                                                         >  2:743963/1‑140 (MQ=255)                                           cTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTcca                                                                                      >  2:1045457/1‑140 (MQ=255)                                           cTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTcca                                                                                      >  1:467024/1‑140 (MQ=255)                                           cTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTcca                                                                                      >  1:412713/1‑140 (MQ=255)                                             gcggcgCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTccagc                                                                                    <  2:739555/140‑1 (MQ=255)                                             gcggcgCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTccagc                                                                                    >  1:543547/1‑140 (MQ=255)                                                 cgcAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAgg                                                                                <  1:1316030/140‑1 (MQ=255)                                                   cAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCg                                                                              <  1:1374588/140‑1 (MQ=255)                                                   cAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCg                                                                              <  2:792771/140‑1 (MQ=255)                                                             cccACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACa                                                                    >  2:1397357/1‑140 (MQ=255)                                                                acaGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGtt                                                                 <  2:387127/140‑1 (MQ=255)                                                                acaGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTACTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGtt                                                                 >  2:928762/1‑140 (MQ=255)                                                                      aCGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCcaca                                                           <  1:1367039/140‑1 (MQ=255)                                                                      aCGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCcaca                                                           <  2:543547/140‑1 (MQ=255)                                                                       cGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACaa                                                          >  1:1506016/1‑140 (MQ=255)                                                                        gTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACaaa                                                         <  2:1370256/140‑1 (MQ=255)                                                                        gTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACaaa                                                         <  2:979723/140‑1 (MQ=255)                                                                          aCCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACt                                                       >  2:707013/1‑140 (MQ=255)                                                                             aGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTccc                                                    <  2:775995/140‑1 (MQ=255)                                                                             aGCGCGGTATCGCGCGTTATGCTCGCCCCAGCCTGACAACAATATACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTGCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCGCAAACTccc                                                    <  2:486920/140‑1 (MQ=255)                                                                               cgcgGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTa                                                  >  1:495837/1‑140 (MQ=255)                                                                               cgcgGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTa                                                  >  1:47976/1‑140 (MQ=255)                                                                                 cgGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAAc                                                <  2:851291/140‑1 (MQ=255)                                                                                  ggTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACg                                               >  1:975529/1‑140 (MQ=255)                                                                                   gTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACgg                                              >  2:957195/1‑140 (MQ=255)                                                                                    tATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGc                                             >  1:819139/1‑140 (MQ=255)                                                                                           cgTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGa                                      <  2:1348984/140‑1 (MQ=255)                                                                                            gTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAg                                     <  2:961489/140‑1 (MQ=255)                                                                                            gTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAg                                     <  2:1311076/140‑1 (MQ=255)                                                                                             ttATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGc                                    <  2:176818/140‑1 (MQ=255)                                                                                             ttATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGc                                    <  1:227320/140‑1 (MQ=255)                                                                                              tATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCg                                   >  2:1375615/1‑140 (MQ=255)                                                                                              tATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCg                                   >  1:568252/1‑140 (MQ=255)                                                                                                 gCTCTCCCCAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGt                                <  1:1418171/140‑1 (MQ=255)                                                                                                      ccccAGCCTGACAACAATAACCCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGt                           <  1:990537/140‑1 (MQ=255)                                                                                                       cccAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTa                          >  2:1186095/1‑140 (MQ=255)                                                                                                       cccAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTa                          <  2:1506016/140‑1 (MQ=255)                                                                                                       cccAGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTa                          <  1:1035251/140‑1 (MQ=255)                                                                                                          aGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGGGCGAGTCAGGTAATg                       >  2:418577/1‑140 (MQ=255)                                                                                                          aGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATg                       >  1:410411/1‑140 (MQ=255)                                                                                                          aGCCTGACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGGAATg                       >  1:65776/1‑140 (MQ=255)                                                                                                               gACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTgag                  >  2:1580613/1‑140 (MQ=255)                                                                                                               gACAACAATAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTgag                  >  2:501755/1‑140 (MQ=255)                                                                                                                       tAAACCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGt          <  1:743963/140‑1 (MQ=255)                                                                                                                         aaCCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCa        <  2:467024/140‑1 (MQ=255)                                                                                                                         aaCCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCa        <  2:311513/140‑1 (MQ=255)                                                                                                                          aCCTCTTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCAc       >  1:296727/1‑140 (MQ=255)                                                                                                                            ctctTCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGc     <  1:1397357/140‑1 (MQ=255)                                                                                                                               ttCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCtg  >  2:1081230/1‑140 (MQ=255)                                                                                                                               ttCCAGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCtg  >  1:98328/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        TTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGACACTGGTAGGGCTGCTCCGGTTGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTG  >  NC_000913/2329408‑2329673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑