Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,429,698	C→G	A181P (GCT→CCT)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,429,698	0	C	G	100.0%	307.1 / NA	80	A181P (GCT→CCT)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (46/34);  total (46/34)

CGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGA  >  NC_000913/2429566‑2429832                                                                                                                                     |                                                                                                                                       cGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATa                                                                                                                                 <  2:475960/140‑1 (MQ=255)   tCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATAcc                                                                                                                               <  1:216697/140‑1 (MQ=255)    cACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCg                                                                                                                              <  2:640218/140‑1 (MQ=255)      cGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTg                                                                                                                            >  1:228330/1‑140 (MQ=255)      cGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTg                                                                                                                            <  2:1412232/140‑1 (MQ=255)          ggAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCg                                                                                                                        <  1:1170158/140‑1 (MQ=255)             aaTCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATa                                                                                                                     <  2:1268140/140‑1 (MQ=255)             aaTCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATa                                                                                                                     >  2:1217840/1‑140 (MQ=255)             aaTCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATa                                                                                                                     >  1:794274/1‑140 (MQ=255)             aaTCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATa                                                                                                                     >  1:546835/1‑140 (MQ=255)               tCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAat                                                                                                                   <  1:890876/140‑1 (MQ=255)               tCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAat                                                                                                                   <  2:559678/140‑1 (MQ=255)                  aaGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAatcat                                                                                                                <  2:1284012/140‑1 (MQ=255)                  aaGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAatcat                                                                                                                <  2:362013/140‑1 (MQ=255)                  aaGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAatcat                                                                                                                <  2:473389/140‑1 (MQ=255)                    gCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCg                                                                                                              <  2:142857/140‑1 (MQ=255)                    gCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCg                                                                                                              <  2:1330985/140‑1 (MQ=255)                    gCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCg                                                                                                              <  2:11666/140‑1 (MQ=255)                         gCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCcaca                                                                                                         <  1:230034/140‑1 (MQ=255)                               cGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCa                                                                                                   >  2:843900/1‑140 (MQ=255)                                      tACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACgcgc                                                                                            >  2:1478702/1‑140 (MQ=255)                                      tACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACgcgc                                                                                            >  2:1329910/1‑140 (MQ=255)                                        cGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGcgccg                                                                                          >  1:1077328/1‑140 (MQ=255)                                         gCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGcgccgc                                                                                         <  1:791778/140‑1 (MQ=255)                                             tCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGACATACGCGCCGCGGAt                                                                                     <  1:1160021/140‑1 (MQ=255)                                                gAAGCGACCATATAATCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTATGGATCGCAGAAAGTAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTaa                                                                                  >  2:207325/1‑140 (MQ=255)                                                     gACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGt                                                                             <  1:1217840/140‑1 (MQ=255)                                                      aCCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGtt                                                                            <  2:1596917/140‑1 (MQ=255)                                                             atTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGgca                                                                     >  1:1247522/1‑140 (MQ=255)                                                                 tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                                                 >  2:1045546/1‑140 (MQ=255)                                                                 tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                                                 >  2:1481245/1‑140 (MQ=255)                                                                 tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                                                 >  1:559457/1‑140 (MQ=255)                                                                 tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                                                 >  1:370954/1‑140 (MQ=255)                                                                 tGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCaa                                                                 >  2:1598655/1‑140 (MQ=255)                                                                  gTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAAt                                                                >  1:815768/1‑140 (MQ=255)                                                                   tACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATg                                                               >  2:1399013/1‑140 (MQ=255)                                                                   tACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATg                                                               >  1:634278/1‑140 (MQ=255)                                                                   tACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATg                                                               >  1:1056438/1‑140 (MQ=255)                                                                   tACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATg                                                               >  2:900211/1‑140 (MQ=255)                                                                    aCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATgg                                                              >  1:1054156/1‑140 (MQ=255)                                                                     cggagctcGTCAATATCACGTTTTCCCAGTCCCAGCGGACGAATCCCGTTTGGATCGCGATAAGGAACCAGACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTATGCAGGGTGTGGCAGCAATGGc                                                             <  2:729090/135‑1 (MQ=255)                                                                        ttCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGc                                                          >  2:1406752/1‑140 (MQ=255)                                                                        ttCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGc                                                          >  1:559010/1‑140 (MQ=255)                                                                         tctcGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCg                                                         <  1:1469999/140‑1 (MQ=255)                                                                             gTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGaaaa                                                     >  2:1370296/1‑140 (MQ=255)                                                                             gTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGaaaa                                                     >  1:303159/1‑140 (MQ=255)                                                                               cAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAata                                                   <  2:228330/140‑1 (MQ=255)                                                                                  tatCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTg                                                >  1:1369569/1‑140 (MQ=255)                                                                                  tatCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTg                                                <  1:150407/140‑1 (MQ=255)                                                                                  tatCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGGGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTg                                                >  2:1480644/1‑140 (MQ=255)                                                                                     cACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCg                                             >  2:1481810/1‑140 (MQ=255)                                                                                       cGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGc                                           >  1:1039349/1‑140 (MQ=255)                                                                                       cGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGc                                           >  2:359290/1‑140 (MQ=255)                                                                                            tCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCa                                      >  1:123495/1‑140 (MQ=255)                                                                                            tCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCa                                      >  1:371720/1‑140 (MQ=255)                                                                                                  tACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGt                                <  2:597/140‑1 (MQ=255)                                                                                                  tACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGt                                >  2:1144497/1‑140 (MQ=255)                                                                                                   aCCAGCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTa                               <  1:1582557/140‑1 (MQ=255)                                                                                                       gCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTgg                           >  2:1389527/1‑140 (MQ=255)                                                                                                       gCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTgg                           >  2:1079954/1‑140 (MQ=255)                                                                                                       gCGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTgg                           >  2:8688/1‑140 (MQ=255)                                                                                                        cGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGc                          <  1:781530/140‑1 (MQ=255)                                                                                                        cGGACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGc                          >  1:1458419/1‑140 (MQ=255)                                                                                                          gACGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCgg                        <  1:719719/140‑1 (MQ=255)                                                                                                            cGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCggga                      >  1:1054583/1‑138 (MQ=255)                                                                                                            cGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGaa                      >  2:1267645/1‑140 (MQ=255)                                                                                                            cGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGaa                      >  1:939420/1‑140 (MQ=255)                                                                                                            cGAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGaa                      <  1:730817/140‑1 (MQ=255)                                                                                                             gAATCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAg                     <  2:559457/140‑1 (MQ=255)                                                                                                              aaTCCCGTTTGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAgg                    >  2:461675/1‑139 (MQ=255)                                                                                                                     tttGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAg             >  1:1422357/1‑140 (MQ=255)                                                                                                                     tttGGATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGGTGTCCAg             >  2:1198776/1‑140 (MQ=255)                                                                                                                         gATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCg         <  2:874521/140‑1 (MQ=255)                                                                                                                         gATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCg         >  2:946413/1‑140 (MQ=255)                                                                                                                         gATCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCg         <  2:411735/140‑1 (MQ=255)                                                                                                                          aTCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGGTGTCCAGCTCGc        >  2:1109811/1‑140 (MQ=255)                                                                                                                           tCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCt       >  1:382191/1‑140 (MQ=255)                                                                                                                           tCGCGGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCt       >  1:953802/1‑140 (MQ=255)                                                                                                                             gcgGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTgg     <  1:1478702/140‑1 (MQ=255)                                                                                                                             gcgGAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTgg     <  2:1200210/140‑1 (MQ=255)                                                                                                                               ggAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCg   <  2:815768/140‑1 (MQ=255)                                                                                                                                gAAAGGAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGa  <  2:1521453/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CGTCACGCAGGAAATCAAAGCCCAGCGTATCGAGCGCTACGCTTTCGGAAGCGACCATATATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGGACGAATCCCGTTTGGATCGCGGAAAGCAACCATACCGTGGCCGATAATCATCGCCACACAGGCATACGCGCCGCGGATTAAGCGGTTTGTGGCAGCAATGGCAGCGAAAATATTGTCGGCTTCCAGCGGGTAGTGGCGGAAGTTGTCCAGCTCGCTGGCGA  >  NC_000913/2429566‑2429832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑