BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,583,424	T→C	T19A (ACC→GCC)	nudK ←	GDP‑mannose hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,583,424	0	T	C	96.6%	306.0 / ‑5.5	89	T19A (ACC→GCC)	nudK	GDP‑mannose hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (44/42); minor base G (3/0); total (47/42)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGAC  >  NC_000913/2583287‑2583560

aCGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGa                                                                                                                                        >  2:144162/1‑140 (MQ=255)

     tCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCCTTGCGCGGGGGATCGTAAGTAATGTTGTGCAGGGCGGAATAg                                                                                                                                   >  2:677396/1‑140 (MQ=255)

       aGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGtt                                                                                                                                 <  2:906856/140‑1 (MQ=255)

        gAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTa                                                                                                                                <  2:422608/140‑1 (MQ=255)

          aCCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGGTAAATTCGCCGTCCTTGCGCGTGAGATCGAAAGAAATGCTGTGCAGGGCGAAATAATTATc                                                                                                                              >  2:589174/1‑140 (MQ=255)

          aCCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATc                                                                                                                              >  1:1153764/1‑140 (MQ=255)

                     tttCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAAttt                                                                                                                   <  1:565009/140‑1 (MQ=255)

                           cGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGGAATGTTGTGCAGGGCGAAATAGTTATCGGGGAGGATTTTGTCtt                                                                                                             >  2:703976/1‑140 (MQ=255)

                           cGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGAAAAATTCTGCGTCTTTGCGCGTGAGAACGTAAATAATGTTGTGCAGGGCGAAAAAGTTATCGGAGAGAATTTTGTCtt                                                                                                             >  2:211998/1‑140 (MQ=255)

                              gttgtACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTaa                                                                                                          <  1:124997/140‑1 (MQ=255)

                                 gtACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGCATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGa                                                                                                       <  2:1157477/140‑1 (MQ=255)

                                   aCAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAgg                                                                                                     <  1:246810/140‑1 (MQ=255)

                                       gaggaTCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGa                                                                                                 >  1:171871/1‑140 (MQ=255)

                                        aggaTCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACCTCGCCGTCTTTGCGCGGGAGATCGTAAGTAATGTTGTGCAGGGCGGAATAGTTATCGGGGAGAATTTTGTCTTTAATGAggggggt                                                                                                >  2:1109193/1‑136 (MQ=255)

                                         ggaTCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGGGAGGTCGTAAGTAATGTTGTTTAGGGCGAAAAAGTTATCGGAGAGCAATTTGTCTTTAATGAggggggtt                                                                                               >  2:871124/1‑135 (MQ=255)

                                           aTCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATttg                                                                                             <  1:144162/140‑1 (MQ=255)

                                           aTCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATttg                                                                                             <  1:329641/140‑1 (MQ=255)

                                            tcgtcgCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGGGAGATCGTAAGTAATGTCGTGCAGGGCGGAATAGTTATCGGGGAGAATTTTGTCTTTAATGAGGGGGATttgt                                                                                            >  2:485111/1‑140 (MQ=255)

                                            tcgtcgCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGGGAGATCGGAAGTAATGTTGTGCAGGGCGGAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATttgt                                                                                            >  2:712956/1‑140 (MQ=255)

                                                 gCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGt                                                                                       <  2:1153764/140‑1 (MQ=255)

                                                 gCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCACGTGAGATCGTAAGTAATGTTGTGCAGGGGGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGt                                                                                       >  1:358820/1‑140 (MQ=255)

                                                  cACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTc                                                                                      <  1:511605/140‑1 (MQ=255)

                                                    ccATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCAt                                                                                    >  1:663504/1‑140 (MQ=255)

                                                    ccATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCAt                                                                                    <  1:677396/140‑1 (MQ=255)

                                                      aTTGTCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATAc                                                                                  <  1:1483196/140‑1 (MQ=255)

                                                      aTTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATAc                                                                                  <  2:416267/140‑1 (MQ=255)

                                                      aTTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATAc                                                                                  <  2:792414/140‑1 (MQ=255)

                                                          ccGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTTCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGAc                                                                              >  1:1105490/1‑140 (MQ=255)

                                                               aTCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGGAATGTTGTGCAGGGCGAAATAGTTATCGGGGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCcac                                                                         >  2:1122981/1‑140 (MQ=255)

                                                                     cacTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGTGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGcgcg                                                                   >  2:1234780/1‑140 (MQ=255)

                                                                       cTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGaa                                                                 <  1:471235/140‑1 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGTGCGTGAGATCGTAAGTAATGTTGTGCAGGGGGAAAAAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGGTTTGTTGCGTCATACCGACTCCCCAGCGCGAAATg                                                              >  2:983457/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGGGGAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:17071/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGGGAGAATTTTGTCTTTAATGAGGGGGGTTTGTTGCGTCATACCGACTCCACAGCGCGGAATg                                                              >  2:933430/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGGTTTGTTGCGTCATACCGACCCCCCAGCGCGGAATg                                                              >  2:27507/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              <  2:1168287/140‑1 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:292313/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:804274/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGGTTTGTTGCGTCATACCGACTCCCCAGCGCGAAATg                                                              >  2:281106/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCCCAGCGCGAAATg                                                              >  2:328292/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:626746/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:1339153/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAAAAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:727361/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTCGTGCAGGGCGAAATAGGTATCGGGGAGAATTTTGTCTTTAATGAGGGGGGTTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:819156/1‑140 (MQ=255)

                                                                          cACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGGAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGGTTTGTTGCGTCATACCGACTCCACAGCGCGAAATg                                                              >  1:985132/1‑140 (MQ=255)

                                                                           aCGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGa                                                             <  1:957910/140‑1 (MQ=255)

                                                                            cGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGaa                                                            <  2:329582/140‑1 (MQ=255)

                                                                               ttATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACaa                                                         <  1:871124/140‑1 (MQ=255)

                                                                               ttATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACaa                                                         <  2:321261/140‑1 (MQ=255)

                                                                               ttATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACaa                                                         <  2:171871/140‑1 (MQ=255)

                                                                                tATGGCGGATAACTTCGCCGTCTTTGCGCGTGGGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAAt                                                        >  1:523329/1‑140 (MQ=255)

                                                                                  tGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTTATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTa                                                      >  1:1560901/1‑140 (MQ=255)

                                                                                  tGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTa                                                      >  1:1227519/1‑140 (MQ=255)

                                                                                  tGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTa                                                      >  1:1472080/1‑140 (MQ=255)

                                                                                  tGGCGGATAACTTCGCCGACTTTGCGCGTGGGATTCTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGGGAATTTTGTCTTTAATGAGGGGGATTTGTTGCGTCATACCGACTCCACAGCGCCAAATGAACAAATa                                                      >  2:93145/1‑140 (MQ=255)

                                                                                                  cGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGATGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGt                                      >  2:659356/1‑140 (MQ=255)

                                                                                                   gTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTc                                     <  1:711901/140‑1 (MQ=255)

                                                                                                      tttGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCggg                                  >  2:1098258/1‑140 (MQ=255)

                                                                                                      tttGCGCGTGAGATCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCggg                                  >  2:189709/1‑140 (MQ=255)

                                                                                                      tttGCGCGTGAGATCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCggg                                  >  2:217697/1‑140 (MQ=255)

                                                                                                         gcgcGTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGttt                               >  2:922998/1‑140 (MQ=255)

                                                                                                           gcgTGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGt                             <  1:983457/140‑1 (MQ=255)

                                                                                                              tGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTg                          >  2:1126460/1‑140 (MQ=255)

                                                                                                              tGAGATCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTg                          >  2:973790/1‑140 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  2:663504/140‑1 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  2:1105490/140‑1 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        >  1:491339/1‑140 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  1:157032/140‑1 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  1:1234780/140‑1 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACGGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  1:485111/140‑1 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        >  2:848685/1‑140 (MQ=255)

                                                                                                                agaTCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTgcg                        <  2:729049/140‑1 (MQ=255)

                                                                                                                  aTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCa                      <  1:271669/140‑1 (MQ=255)

                                                                                                                  aTCGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAACGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCa                      <  1:1109193/140‑1 (MQ=255)

                                                                                                                  aTCGTAAGTAATGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCa                      <  1:877829/140‑1 (MQ=255)

                                                                                                                    cGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAgg                    <  1:93145/140‑1 (MQ=255)

                                                                                                                    cGTAAGTAATGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAgg                    <  2:722035/140‑1 (MQ=255)

                                                                                                                     gtaagtaaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGc                   <  2:336123/140‑1 (MQ=255)

                                                                                                                        agtaaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTAt                <  1:121779/140‑1 (MQ=255)

                                                                                                                        agtaaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTAt                <  2:1472080/140‑1 (MQ=255)

                                                                                                                        agtaaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTAt                >  1:538826/1‑140 (MQ=255)

                                                                                                                           aaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAg             <  2:523329/140‑1 (MQ=255)

                                                                                                                           aaTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAg             >  1:339667/1‑140 (MQ=255)

                                                                                                                            aTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGt            >  1:1256403/1‑140 (MQ=255)

                                                                                                                            aTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGt            <  2:727361/140‑1 (MQ=255)

                                                                                                                            aTGTTGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGt            >  1:712811/1‑140 (MQ=255)

                                                                                                                            aTGTCGTGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGt            >  2:870497/1‑140 (MQ=255)

                                                                                                                             tgttgtGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTg           >  1:874663/1‑140 (MQ=255)

                                                                                                                               ttgtGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTgag         >  2:636542/1‑140 (MQ=255)

                                                                                                                               ttgtGCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTgag         >  1:282863/1‑140 (MQ=255)

                                                                                                                                 gtgCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAt       >  1:362304/1‑140 (MQ=255)

                                                                                                                                  tgCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAtt      >  2:1322270/1‑140 (MQ=255)

                                                                                                                                  tgCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAtt      >  1:744204/1‑140 (MQ=255)

                                                                                                                                  tgCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAtt      >  1:526143/1‑140 (MQ=255)

                                                                                                                                  tgCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAtt      >  1:334069/1‑140 (MQ=255)

                                                                                                                                   gCAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGAttt     >  2:646996/1‑140 (MQ=255)

                                                                                                                                    cAGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTg    >  1:477954/1‑140 (MQ=255)

                                                                                                                                     aGGGCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGa   <  2:209761/140‑1 (MQ=255)

                                                                                                                                      gggCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGAc  <  1:67773/140‑1 (MQ=255)

                                                                                                                                      gggCGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGAc  <  1:933430/140‑1 (MQ=255)

ACGAATCAGAACCACGGTCTTTTTCTTCGTGTTGTACAGGAGGATCGTCGCACCATTGCCGCGATCGTACACTTCACGTTTATGGCGGATAACTTCGCCGTCTTTGCGCGTGAGATCGTAAGTAATGTTGTGCAGGGTGAAATAGTTATCGGAGAGAATTTTGTCTTTAATGAGGGTGATTTGTTGCGTCATACCGACTCCACAGCGCGAAATGAACAATTATCTTACGCTGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGAC  >  NC_000913/2583287‑2583560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`