Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,634,926	T→C	V232A (GTT→GCT)	xseA →	exodeoxyribonuclease VII subunit XseA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,634,926	0	T	C	100.0%	302.9 / NA	81	V232A (GTT→GCT)	xseA	exodeoxyribonuclease VII subunit XseA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/44);  total (37/44)

TCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGAC  >  NC_000913/2634789‑2635061                                                                                                                                          |                                                                                                                                        tCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTg                                                                                                                                       >  1:739550/1‑140 (MQ=255) tCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTg                                                                                                                                       >  1:465087/1‑140 (MQ=255)  cgcgCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGt                                                                                                                                      >  1:686570/1‑140 (MQ=255)     gcCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAg                                                                                                                                   >  2:291684/1‑140 (MQ=255)      ccATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAgc                                                                                                                                  <  1:1412463/140‑1 (MQ=255)        aTTGAACTGGCGAATCAGCGCTATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAgcgc                                                                                                                                <  1:853878/140‑1 (MQ=255)          tGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCg                                                                                                                              >  1:1250986/1‑140 (MQ=255)           gAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGt                                                                                                                             <  2:605390/140‑1 (MQ=255)           gAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGt                                                                                                                             <  2:282761/140‑1 (MQ=255)               tGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCggg                                                                                                                         <  2:202945/140‑1 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       >  1:1042825/1‑140 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       >  2:1335480/1‑140 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       <  1:97841/140‑1 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       >  1:536345/1‑140 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       <  1:511097/140‑1 (MQ=255)                 gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa                                                                                                                       >  1:28192/1‑140 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  2:951248/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  >  1:1140428/1‑140 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  2:1021736/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  1:530225/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  1:318935/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  1:1395409/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  <  1:508947/140‑1 (MQ=255)                      tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga                                                                                                                  >  2:570559/1‑140 (MQ=255)                       cAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGAc                                                                                                                 <  2:110561/140‑1 (MQ=255)                           gcAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGAt                                                                                                             >  1:945784/1‑140 (MQ=255)                              aTGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGAtgtg                                                                                                          <  1:1175891/140‑1 (MQ=255)                                  gTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCa                                                                                                      >  1:1223637/1‑140 (MQ=255)                                   tGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCAt                                                                                                     <  1:1125989/140‑1 (MQ=255)                                    gCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCAtt                                                                                                    >  1:506408/1‑140 (MQ=255)                                    gCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCAtt                                                                                                    <  1:691728/140‑1 (MQ=255)                                    gCGACGTATTGATCGTCGCGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTACCGACGAACGCGTAGCGCGGGCGACTTTTACCAGCCGCATTCCGGCTGTCTGCGCCGTAGGGCATGAGACGGATGTGACCAtt                                                                                                    >  2:1150604/1‑140 (MQ=255)                                         gTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCgg                                                                                               >  2:934220/1‑139 (MQ=255)                                           aTTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGAtt                                                                                             >  1:566349/1‑140 (MQ=255)                                             tGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGAtttt                                                                                           >  2:1583800/1‑140 (MQ=255)                                             tGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGAtttt                                                                                           >  2:1262378/1‑140 (MQ=255)                                                tcgtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgtt                                                                                        <  1:1374697/140‑1 (MQ=255)                                                  gtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGc                                                                                      >  1:1040393/1‑140 (MQ=255)                                                  gtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGc                                                                                      >  1:1456044/1‑140 (MQ=255)                                                   tcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACTGATGTGACCATTGCCGATTTTGTTGcc                                                                                     >  1:279047/1‑140 (MQ=255)                                                   tcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGcc                                                                                     <  1:1552535/140‑1 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  2:487737/1‑140 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  1:1295366/1‑140 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  2:909564/1‑140 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  1:1296706/1‑140 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  1:561981/1‑140 (MQ=255)                                                    cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg                                                                                    >  1:315109/1‑140 (MQ=255)                                                          cggcggcggTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCtgc                                                                              >  2:20469/1‑140 (MQ=255)                                                           ggcggtggTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCtgcg                                                                             <  1:26823/140‑1 (MQ=255)                                                             cggcggTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGACATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCtgcgtg                                                                           >  2:604004/1‑140 (MQ=255)                                                                             gATTTATGGAGTTTTAACGACGAACGCGTGGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgc                                                           <  2:566349/140‑1 (MQ=255)                                                                             gATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgc                                                           <  2:40439/140‑1 (MQ=255)                                                                              aTTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgcc                                                          <  2:1303403/140‑1 (MQ=255)                                                                                ttATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgccgc                                                        <  1:911785/140‑1 (MQ=255)                                                                                ttATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgccgc                                                        <  2:1027750/140‑1 (MQ=255)                                                                                ttATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgccgc                                                        <  1:1047241/140‑1 (MQ=255)                                                                                ttATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgccgc                                                        <  2:536345/140‑1 (MQ=255)                                                                                      aGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGa                                                  <  1:260647/140‑1 (MQ=255)                                                                                        ttttAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAag                                                >  2:737437/1‑140 (MQ=255)                                                                                        ttttAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAag                                                >  1:459982/1‑140 (MQ=255)                                                                                          ttAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAagta                                              >  1:1567325/1‑140 (MQ=255)                                                                                          ttAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAagta                                              <  2:1140428/140‑1 (MQ=255)                                                                                           tAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAagtag                                             <  2:1250986/140‑1 (MQ=255)                                                                                               gacgaACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAg                                         <  1:478433/140‑1 (MQ=255)                                                                                               gacgaACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAg                                         <  1:741990/140‑1 (MQ=255)                                                                                                    aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa                                    <  2:1575570/140‑1 (MQ=255)                                                                                                    aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa                                    <  2:1068449/140‑1 (MQ=255)                                                                                                          aGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGc                              <  2:28192/140‑1 (MQ=255)                                                                                                          aGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGc                              <  2:446469/140‑1 (MQ=255)                                                                                                            cgcgGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCaa                            <  2:945784/140‑1 (MQ=255)                                                                                                              cgGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAaga                          >  1:470370/1‑140 (MQ=255)                                                                                                                ggCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGt                        >  2:505534/1‑140 (MQ=255)                                                                                                                 gCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGtt                       <  1:1333623/140‑1 (MQ=255)                                                                                                                 gCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGtt                       <  2:1223637/140‑1 (MQ=255)                                                                                                                 gCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGtt                       >  1:369438/1‑140 (MQ=255)                                                                                                                       tttACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTgcg                 <  2:487869/140‑1 (MQ=255)                                                                                                                       tttACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTgcg                 <  2:315109/140‑1 (MQ=255)                                                                                                                       tttACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTgcg                 <  1:291684/140‑1 (MQ=255)                                                                                                                            cAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAgg            <  2:739550/140‑1 (MQ=255)                                                                                                                            cAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAgg            <  1:947353/140‑1 (MQ=255)                                                                                                                               ccGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGc         >  2:1591388/1‑140 (MQ=255)                                                                                                                               ccGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGc         >  1:959802/1‑140 (MQ=255)                                                                                                                                      ccGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGAc  <  1:4392/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCGCGCCATTGAACTGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGAC  >  NC_000913/2634789‑2635061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑