Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,646,693	C→T	W211* (TGG→TGA)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,646,693	0	C	T	100.0%	192.4 / NA	58	W211* (TGG→TGA)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/14);  total (44/14)

GTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCC  >  NC_000913/2646557‑2646827                                                                                                                                         |                                                                                                                                       gTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCaa                                                                                                                                     <  2:171605/140‑1 (MQ=255)      aGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATc                                                                                                                                <  1:1193458/140‑1 (MQ=255)      aGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATc                                                                                                                                <  1:896538/140‑1 (MQ=255)            ggCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCg                                                                                                                          >  2:788932/1‑140 (MQ=255)                cttGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGa                                                                                                                      >  1:804653/2‑140 (MQ=255)                        ggggCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGACTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTggg                                                                                                              >  1:368136/1‑140 (MQ=255)                                    aTGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCaa                                                                                                  >  1:1566442/1‑140 (MQ=255)                                     tGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCaaa                                                                                                 >  2:969958/1‑140 (MQ=255)                                           cttcCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGcc                                                                                           >  2:1287469/1‑140 (MQ=255)                                                   gcctctTTTCCCTGCTCACGGGCCCACACACCTTGCACGGCCATCCGTTCGAGCCCTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgc                                                                                   <  2:1360722/138‑1 (MQ=255)                                                   gACTCTTTTCCCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTCCGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgc                                                                                   <  1:250792/140‑1 (MQ=255)                                                   gACTCTTTTACCTGCTCACGGGACCACCCACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgc                                                                                   <  2:209984/140‑1 (MQ=255)                                                   gACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgc                                                                                   <  1:66842/140‑1 (MQ=255)                                                   gACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCAGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgc                                                                                   >  1:1534266/1‑140 (MQ=255)                                                            aCCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGa                                                                          >  1:835968/1‑140 (MQ=255)                                                             ccTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGaa                                                                         <  1:648771/140‑1 (MQ=255)                                                                gCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAg                                                                      >  2:1492127/1‑140 (MQ=255)                                                                gCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAg                                                                      >  1:672208/1‑140 (MQ=255)                                                                  tCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGGATAGCt                                                                    >  2:1237192/1‑140 (MQ=255)                                                                  tCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCt                                                                    >  2:1078384/1‑140 (MQ=255)                                                                      gggACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTaaa                                                                >  2:1065686/1‑140 (MQ=255)                                                                           cacacaCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGc                                                           >  2:450377/1‑140 (MQ=255)                                                                           cacacaCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGc                                                           >  2:551262/1‑140 (MQ=255)                                                                           cacacaCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGc                                                           >  2:635352/1‑140 (MQ=255)                                                                            ccacccCTTGCGGGGCCATCCGTCCGGGCGCTTTATTACGCGCGGCTTCGGCGCGCCCCGGCCAACGATCCGGGGGAAGGCGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCa                                                          <  2:659563/139‑1 (MQ=255)                                                                                cccTTGCCCGGCCATCCGTTCGAGCGCTTTATTCCGCGCGGCTTCGGCCCGCTCCGGTCAACGATCGGGGCGAAGACGGTTGGGCGCTTGCGGCAAACCCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCg                                                      <  2:203199/139‑1 (MQ=255)                                                                                  cTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAt                                                    >  2:335046/1‑140 (MQ=255)                                                                                  cTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAt                                                    >  2:136228/1‑140 (MQ=255)                                                                                  cTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAt                                                    >  1:158421/1‑140 (MQ=255)                                                                                  cTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAt                                                    >  2:266292/1‑140 (MQ=255)                                                                                    tGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAttt                                                  >  1:666723/1‑140 (MQ=255)                                                                                    tGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAttt                                                  >  2:1237262/1‑140 (MQ=255)                                                                                     gCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAtttt                                                 >  1:634355/1‑140 (MQ=255)                                                                                     gCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGAtttt                                                 >  2:1385367/1‑140 (MQ=255)                                                                                          gCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCgag                                            >  2:47116/1‑140 (MQ=255)                                                                                          gCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCgag                                            >  1:653250/1‑140 (MQ=255)                                                                                          gCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCgag                                            >  2:471907/1‑140 (MQ=255)                                                                                            cATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGAt                                          >  1:1527735/1‑140 (MQ=255)                                                                                            cATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGAt                                          >  2:165178/1‑140 (MQ=255)                                                                                              tCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATaa                                        >  1:1541565/1‑140 (MQ=255)                                                                                              tCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATaa                                        >  1:800463/1‑140 (MQ=255)                                                                                              tCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATaa                                        >  1:1217642/1‑140 (MQ=255)                                                                                                cGTTCGAGCTCTTTATTACGCGCGGCTCCGGCACGCCCCGGTCAACGATCCGGGCGAAGACGGTTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGc                                      <  2:717062/140‑1 (MQ=255)                                                                                                   tcaggCACTTTTTTACGCGCGGCGTGGGCCCGCTCCGTGCAACGTTCCGGGCGATGATGGCCGGGCGCTTGCGTCAAATCCGCCAGCATTGCCGCCGCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCa                                   <  2:1121645/136‑1 (MQ=255)                                                                                                   ccGAGCACTTTTTTACGCGCGGCTTGGGCACGCTCCGGTCAACGACCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCa                                   <  2:568030/139‑1 (MQ=255)                                                                                                     gAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAAc                                 >  1:279728/1‑140 (MQ=255)                                                                                                      ggCACTTTATTAGCCGGGACTGCGGCACGCCCCGGTCAACGATCCGGGCAAAGACGGCGGTGCGCTGGGGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACt                                <  2:870736/139‑1 (MQ=255)                                                                                                        cACTTTATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTg                              >  2:421484/1‑140 (MQ=255)                                                                                                           tttATTCCGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCg                           <  2:1295209/140‑1 (MQ=255)                                                                                                           tttATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCg                           <  2:1566442/140‑1 (MQ=255)                                                                                                           tttATTACGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCg                           <  1:251112/140‑1 (MQ=255)                                                                                                                tgcgcgggGCTTCGGCCCGCTCCGGTCAACGATCCGGGGGAAGACGGGTGGGCGCTTGGGCCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGA‑TTTCCGAGATAAGCCCAACTTGCCGCACCGa                     <  2:268348/138‑1 (MQ=255)                                                                                                                taCGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCTCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCg                      <  2:804653/140‑1 (MQ=255)                                                                                                                taCGCGCGGCTCCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCg                      <  1:477472/140‑1 (MQ=255)                                                                                                                 aCGCGCGGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGa                     >  1:1307225/1‑140 (MQ=255)                                                                                                                   gcgcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATc                   >  1:113102/1‑140 (MQ=255)                                                                                                                   gcgcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATc                   <  1:788932/140‑1 (MQ=255)                                                                                                                   gcgcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATc                   <  1:1237262/140‑1 (MQ=255)                                                                                                                     gcgGCTTCGGCCCGCCCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTGGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATccc                 <  2:578404/140‑1 (MQ=255)                                                                                                                     gcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATccc                 >  1:242511/1‑140 (MQ=255)                                                                                                                     gcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATccc                 >  2:84025/1‑140 (MQ=255)                                                                                                                     gcgGCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATccc                 >  2:981010/1‑140 (MQ=255)                                                                                                                       ggCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCt               >  2:1406034/1‑140 (MQ=255)                                                                                                                        gCTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTg              >  2:671048/1‑140 (MQ=255)                                                                                                                         cTTCGGCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGc             >  2:232464/1‑140 (MQ=255)                                                                                                                          tccGGCACGCTCCGGTCAACGATCGGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCa            <  1:1097574/138‑1 (MQ=255)                                                                                                                             ggCACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACg         <  1:1385367/140‑1 (MQ=255)                                                                                                                               cACGCTCGGGTCAACGATCCGGGCGAAGCCGGCGGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTa       <  2:1217642/140‑1 (MQ=255)                                                                                                                               cACGCTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTa       <  1:335046/140‑1 (MQ=255)                                                                                                                                   cTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGc   >  2:189208/1‑140 (MQ=255)                                                                                                                                   cTCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGc   >  2:1548247/1‑140 (MQ=255)                                                                                                                                    tCCGGTCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGcc  >  2:334492/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTAAATTCGCAGGCGATTTTCCGAGATAAGCCCAACTTGCCGCACCGATCCCCTGCAACGTACCGCC  >  NC_000913/2646557‑2646827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑