Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,686	T→C	R18R (CGT→CGC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,686	0	T	C	100.0%	271.5 / NA	71	R18R (CGT→CGC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/34);  total (37/34)

TAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGC  >  NC_000913/3222550‑3222817                                                                                                                                         |                                                                                                                                    tAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCcgcgcgcg                                                                                                                                  >  2:840001/1‑140 (MQ=255)     aaTTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCCCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACt                                                                                                                              <  1:1469026/140‑1 (MQ=255)          tACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACtttttt                                                                                                                         <  2:1319082/140‑1 (MQ=255)             tAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCa                                                                                                                      <  2:116035/140‑1 (MQ=255)               aaCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCata                                                                                                                    <  2:978627/140‑1 (MQ=255)               aaCTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCata                                                                                                                    <  1:1208524/140‑1 (MQ=255)                 cTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATg                                                                                                                  >  2:1484741/1‑140 (MQ=255)                     ccAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTc                                                                                                              <  2:251858/140‑1 (MQ=255)                       aGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACACTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTg                                                                                                            >  1:43103/1‑140 (MQ=255)                         cAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGtt                                                                                                          >  2:656442/1‑140 (MQ=255)                            ttACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTgcg                                                                                                       >  2:82561/1‑140 (MQ=255)                            ttACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTgcg                                                                                                       >  1:1305987/1‑140 (MQ=255)                             tACACAAACTTCCATCTCCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCCCCCTCGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTgcgc                                                                                                      <  2:778357/140‑1 (MQ=255)                             tACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTgcgc                                                                                                      <  2:689719/140‑1 (MQ=255)                              acacAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCa                                                                                                     >  1:894497/1‑140 (MQ=255)                              acacAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCa                                                                                                     >  1:603437/1‑140 (MQ=255)                              acacAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCa                                                                                                     >  2:1444447/1‑140 (MQ=255)                                   aaCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAgcgc                                                                                                <  1:230027/140‑1 (MQ=255)                                   aaCTACCATCACCATGAATGATTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAgcgc                                                                                                <  2:999501/140‑1 (MQ=255)                                    aCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGcgcg                                                                                               <  2:24171/140‑1 (MQ=255)                                      tACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTa                                                                                             >  1:1339135/1‑140 (MQ=255)                                            cACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTg                                                                                       <  1:1000704/140‑1 (MQ=255)                                            cACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTg                                                                                       <  2:299373/140‑1 (MQ=255)                                              ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGcc                                                                                     >  1:254408/1‑140 (MQ=255)                                              ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGcc                                                                                     >  2:1594541/1‑140 (MQ=255)                                              ccATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTAGGAAAACATTCAGCTCACCCACGAAAACCGACTTGTGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGcc                                                                                     >  2:398733/1‑140 (MQ=255)                                                aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcg                                                                                   >  2:1142161/1‑140 (MQ=255)                                                aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcg                                                                                   >  1:595612/1‑140 (MQ=255)                                                aTGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCAAATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcg                                                                                   >  2:289467/1‑140 (MQ=255)                                                  gAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcgcg                                                                                 >  2:1039721/1‑140 (MQ=255)                                                  gAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCcgcg                                                                                 >  1:1158276/1‑140 (MQ=255)                                                       gTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAAcc                                                                            >  1:1520009/1‑140 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          >  1:1229686/1‑140 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          >  1:1106772/1‑140 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          >  1:210620/1‑140 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          <  1:50011/140‑1 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          <  1:1484741/140‑1 (MQ=255)                                                         tCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACcag                                                                          >  1:1489929/1‑140 (MQ=255)                                                             aTTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCagcagc                                                                      >  2:143577/1‑140 (MQ=255)                                                                 ctctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGt                                                                  >  1:10081/1‑140 (MQ=255)                                                                   ctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGttt                                                                <  1:857866/140‑1 (MQ=255)                                                                   ctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGttt                                                                <  2:382879/140‑1 (MQ=255)                                                                   ctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGttt                                                                <  2:602362/140‑1 (MQ=255)                                                                   ctctACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGttt                                                                <  1:1072678/140‑1 (MQ=255)                                                                           ggAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCtt                                                        >  1:517773/1‑140 (MQ=255)                                                                           ggAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCtt                                                        >  2:332693/1‑140 (MQ=255)                                                                              ggCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAg                                                     <  1:840001/140‑1 (MQ=255)                                                                                cccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCg                                                   >  1:605457/1‑140 (MQ=255)                                                                                cccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCg                                                   >  2:379898/1‑140 (MQ=255)                                                                                         tCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGa                                          >  1:255537/1‑140 (MQ=255)                                                                                          cGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa                                         >  1:838447/1‑140 (MQ=255)                                                                                          cGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa                                         >  1:1119353/1‑140 (MQ=255)                                                                                             tGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAAttt                                      >  1:1034582/1‑140 (MQ=255)                                                                                              gggAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTc                                     >  1:1078337/1‑140 (MQ=255)                                                                                               ggAAAACATTCAGCTCACCCACGAAAACCGACTTGTGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTcc                                    <  1:398733/140‑1 (MQ=255)                                                                                                       ttCAGCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtttttt                            >  1:698971/1‑140 (MQ=255)                                                                                                           gCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAcc                        <  2:875148/140‑1 (MQ=255)                                                                                                           gCTCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCAAATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAcc                        <  1:289467/140‑1 (MQ=255)                                                                                                             tCACCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTTTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCAt                      <  1:1110141/140‑1 (MQ=255)                                                                                                               aCCCACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATcc                    >  2:1032457/1‑140 (MQ=255)                                                                                                                cccACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCg                   <  1:1005189/140‑1 (MQ=255)                                                                                                                  cACGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCt                 <  2:210620/140‑1 (MQ=255)                                                                                                                   aCGAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTg                <  1:857529/140‑1 (MQ=255)                                                                                                                     gAAAACCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCa              <  2:254408/140‑1 (MQ=255)                                                                                                                      aaaaCCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGcta             >  1:1346049/1‑138 (MQ=255)                                                                                                                       aaaCCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAg            <  2:43103/140‑1 (MQ=255)                                                                                                                       aaaCCGACTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAg            <  1:82561/140‑1 (MQ=255)                                                                                                                             aCTTGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAg      <  1:332693/140‑1 (MQ=255)                                                                                                                               ttGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGaa    <  2:1229686/140‑1 (MQ=255)                                                                                                                               ttGCGCCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGaa    <  2:1339135/140‑1 (MQ=255)                                                                                                                                 gcgcCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGc  >  1:254952/1‑140 (MQ=255)                                                                                                                                 gcgcCGCGCGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGc  <  2:1489929/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGC  >  NC_000913/3222550‑3222817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑